Combinatorial peptide library protocols /

Combinatorial peptide library protocols /

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Bibliographic Details
Imprint:	Totowa, N.J. : Humana Press, ©1998.
Description:	1 online resource (xiii, 313 pages) : illustrations (some color)
Language:	English
Series:	Methods in molecular biology ; v. 87 Methods in molecular biology (Clifton, N.J.) ; v. 87.
Subject:	Peptides -- Synthesis. Combinatorial chemistry. Protein binding. Peptide Library. Peptides -- chemical synthesis. Molecular Sequence Data. Carrier Proteins. Combinatorial chemistry. Peptides -- Synthesis. Protein binding.
Format:	E-Resource Book
URL for this record:	http://pi.lib.uchicago.edu/1001/cat/bib/11170672

Hidden Bibliographic Details
Other authors / contributors:	Cabilly, Shmuel.
ISBN:	9781592595716 1592595715 1280836873 9781280836879 9786610836871 6610836876 0896033929 9780896033924
Notes:	Includes bibliographical references and index. English. Print version record.
Summary:	Shmuel Cabilly presents in Combinatorial Peptide Library Protocols a collection of new and unique techniques for the construction and use of peptide libraries. These powerful methods-often detailed here by their pioneers-include protocols for the chemical synthesis of peptide libraries, for constructing peptide libraries that are displayed on the surface of filamentous phage or bacteria, and for the rapid screening of these libraries for molecules with biospecific properties. Additional methods permit identifying specific enzyme substrates, investigating the recognition spectra of various binding proteins, epitope mapping, and identifying mimotopes. Combinatorial Peptide Library Protocols offers novice and experienced investigators alike the ability to select molecules from a randomized pool having specific biological activities. Its state-of-the-art techniques, combined with clear step-by-step instructions, make this book an essential tool in the selection of peptides suitable for drug development.
Other form:	Print version: Combinatorial peptide library protocols. Totowa, N.J. : Humana Press, ©1998 0896033929
Standard no.:	10.1385/0896033929.

Description
Summary:	During the course of evolution, an imbalance was created between the rate of vertebrate genetic adaptation and that of the lower forms of living organisms, such as bacteria and viruses. This imbalance has given the latter the advantage of generating, relatively quickly, molecules with unexpected structures and features that carry a threat to vertebrates. To compensate for their weakness, vertebrates have accelerated their own evolutionary processes, not at the level of whole organism, but in specialized cells containing the genes that code for antibody molecules or for T-cell receptors. That is, when an immediate requirement for molecules capable of specific interactions arose, nature has preferred to speed up the mode of Darwinian evolution in pref- ence to any other approach (such as the use of X-ray diffraction studies and computergraphic analysis). Recently, Darwinian rules have been adapted for test tube research, and the concept of selecting molecules having particular characteristics from r- dom pools has been realized in the form of various chemical and biological combinatorial libraries. While working with these libraries, we noticed the interesting fact that when combinatorial libraries of oligopeptides were allowed to interact with different selector proteins, only the actual binding sites of these proteins showed binding properties, whereas the rest of the p- tein surface seemed "inert. " This seemingly common feature of protein- having no extra potential binding sites--was probably selected during evolution in order to minimize nonspecific interactions with the surrounding milieu.
Physical Description:	1 online resource (xiii, 313 pages) : illustrations (some color)
Bibliography:	Includes bibliographical references and index.
ISBN:	9781592595716 1592595715 1280836873 9781280836879 9786610836871 6610836876 0896033929 9780896033924