Combinatorial peptide library protocols /

Combinatorial peptide library protocols /

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Bibliographic Details
Imprint:Totowa, N.J. : Humana Press, ©1998.
Description:1 online resource (xiii, 313 pages) : illustrations (some color)
Language:English
Series:Methods in molecular biology ; v. 87
Methods in molecular biology (Clifton, N.J.) ; v. 87.
Subject:
Peptides -- Synthesis.
Combinatorial chemistry.
Protein binding.
Peptide Library.
Peptides -- chemical synthesis.
Molecular Sequence Data.
Carrier Proteins.
Combinatorial chemistry.
Peptides -- Synthesis.
Protein binding.
Format: E-Resource Book
URL for this record:http://pi.lib.uchicago.edu/1001/cat/bib/11170672
Hidden Bibliographic Details
Other authors / contributors:Cabilly, Shmuel.
ISBN:9781592595716
1592595715
1280836873
9781280836879
9786610836871
6610836876
0896033929
9780896033924
Notes:Includes bibliographical references and index.
English.
Print version record.
Summary:Shmuel Cabilly presents in Combinatorial Peptide Library Protocols a collection of new and unique techniques for the construction and use of peptide libraries. These powerful methods-often detailed here by their pioneers-include protocols for the chemical synthesis of peptide libraries, for constructing peptide libraries that are displayed on the surface of filamentous phage or bacteria, and for the rapid screening of these libraries for molecules with biospecific properties. Additional methods permit identifying specific enzyme substrates, investigating the recognition spectra of various binding proteins, epitope mapping, and identifying mimotopes. Combinatorial Peptide Library Protocols offers novice and experienced investigators alike the ability to select molecules from a randomized pool having specific biological activities. Its state-of-the-art techniques, combined with clear step-by-step instructions, make this book an essential tool in the selection of peptides suitable for drug development.
Other form:Print version: Combinatorial peptide library protocols. Totowa, N.J. : Humana Press, ©1998 0896033929
Standard no.:10.1385/0896033929.
Table of Contents:
  • Synthesis of a one-bead one-compound combinatorial peptide library / Kit S. Lam, Michal Lebl
  • Enzyme-linked calorimetric screening of a one-bead one-compound combinatorial library / Kit S. Lam
  • Synthesis and screening of positional scanning combinatorial libraries / Colette T. Dooley, Richard A. Houghten
  • Synthesis and screening of peptide libraries on continuous cellulose membrane supports / Achim Kramer, Jens Schneider-Mergener
  • Peralkylation : "libraries from libraries" : chemical transformation of synthetic combinatorial libraries / John M. Ostresh, Barbara Dörner, Richard A. Houghten
  • Introduction to combinatorial solid-phase assays for enzyme activity and inhibition / Morten Meldal
  • Preparation of biocompatible resins for library syntheses / Morten Meldal
  • Intramolecular fluorescence-quenched substrate libraries / Morten Meldal
  • The solid-phase enzyme inhibitor library assay / Morten Meldal
  • Determination of peptide substrate motifs for protein kinases using a "one-bead one-compound" combinatorial library approach / Kit S. Lam
  • The use of peptide library for the determination of kinase peptide substrates / Zhou Songyang, Lewis C. Cantley
  • Analysis of protein kinase substrate specificity by the use of peptide libraries on cellulose paper (SPOT-Method) / Werner J. Tegge, Ronald Frank
  • Generation of multiuse peptide libraries for functional screenings / Channa K. Jayawickreme, Shiranthi P. Jayawickreme, Michael R. Lerner
  • Functional screening of multiuse peptide libraries using melanophore bioassay / Channa K. Jayawickreme, Shiranthi P. Jayawickreme, Michael R. Lerner
  • The basic structure of filamentous phage and its use in the display of combinatorial peptide libraries / Shmuel Cabilly
  • Construction and use of a 20-mer phage display epitope library / Baruch Stern, Jonathan M. Gershoni
  • Construction of disulfide-constrained random peptide libraries displayed on phage coat protein VIII / Alessandra Luzzago, Franco Felici
  • Conformational mimicry through random constraints plus affinity selection / Guangming Zhong
  • The use of combinatorial libraries to identify ligands that interact with surface receptors in living cells / Shmuel Cabilly, Judith Heldman, Eliahu Heldman, Ephraim Katchalski-Katzir
  • Screening phage display peptide libraries on nitrocellulose membranes / Shmuel Cabilly, Judith Heidman, Ephraim Katchalski-Katzir
  • Identification of disease-specific epitopes / Antonella Folgori, Alessandra Luzzago, Paolo Monaci, Alfredo Nicosia, Riccardo Cortese, Franco Felici
  • Identification of peptide ligands for the antigen binding receptor expressed on human B-cell lymphomas / Markus F. Renschler, William J. Dower, Ronald Levy
  • Major histocompatibility complex allele-specific peptide libraries and identification of T-cell mimotopes / Marc A. Gavin, Michael J. Bevan
  • Phage display of peptide libraries on protein scaffolds / Henry B. Lowman
  • Displaying libraries of conformationally constrained peptides on the surface of Escherichia coli as flagellin fusions / Zhijan Lu, Brian C. Tripp, John M. McCoy
  • Combinatorial peptide library as an immunogen / J. Estaquier, J.-C. Ameisen, C. Auriault, C. Boutillon, H. Gras-Masse, A. Tartar.

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