In vitro mutagenesis protocols /
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| Imprint: | Totowa, N.J. : Humana Press, ©1996. |
|---|---|
| Description: | 1 online resource (xiv, 390 pages) : illustrations |
| Language: | English |
| Series: | Methods in molecular biology ; v. 57 Methods in molecular biology (Clifton, N.J.) ; v. 57. |
| Subject: | |
| Format: | E-Resource Book |
| URL for this record: | http://pi.lib.uchicago.edu/1001/cat/bib/11171105 |
Table of Contents:
- Site-directed mutagenesis using positive antibiotic selection / Richard N. Bohnsack
- In vitro site-directed mutagenesis using the unique restriction site elimination (USE) method / Li Zhu
- Site-directed mutagenesis using double-stranded plasmid DNA templates / Jeffrey Braman, Carol Papworth, Alan Greener
- Site-directed mutagenesis using a uracil-containing phagemid template / Christian Hagemeier
- Oligonucleotide-directed mutagenesis using an improved phosphorothioate approach / Susan J. Dale, Ian R. Felix
- Analysis of point mutations by use of amber stop codon suppression / Scott A. Lesley
- A simple method for site-directed mutagenesis with double-stranded plasmid DNA / Derhsing Lai, Sidney Pestka
- Double-stranded DNA site-directed mutagenesis / Stéphane Viville
- Solid-phase in vitro mutagenesis using a plasmid DNA template / Roy Edward
- Targeted mutagenesis mediated by the triple helix formation / Peter M. Glazer, Gun Wang, Pamela A. Havre, Edward J. Gunther
- A universal nested deletion method using an arbitrary primer and elimination of a unique restriction site / Li Zhu, Ann E. Holtz
- Ordered deletions using exonuclease III / Denise Clark, Steven Henikoff
- Ligase chain reaction for site-directed in vitro mutagenesis / Gerard J. Rouwendal, Emil J. Wolbert, Lute-Harm Zwiers, Jan Springer
- PCR-based site-directed mutagenesis / Atsushi Shimada
- In vitro recombination and mutagenesis by overlap extension PCR / Robert J. Pogulis, Abbe N. Vallejo, Larry R. Pease
- Site-directed mutagenesis using overlap extension PCR / Ashok Aiyar, Yan Xiang, Jonathan Leis
- Modification of the overlap extension method for extensive mutagenesis on the same template / Ivan Mikaelian, Alain Sergeant
- Site-directed mutagenesis in vitro by megaprimer PCR / Sailen Barik
- Using PCR for rapid site-specific mutagenesis in large plasmids / Brynmor A. Watkins, Marvin S. Reitz
- PCR-assisted mutagenesis for site-directed insertion/deletion of large DNA segments / Daniel C. Tessier, David Y. Thomas
- Site-directed mutagenesis using a rapid PCR-based method / Gina L. Costa, John C. Bauer, Barbara McGowan, Mila Angert, Michael P. Weiner
- A simple method to introduce internal deletions or mutations into any position of a target DNA sequence / Marjana Tomic-Canic, Françoise Bernerd, Miroslav Blumenberg
- A simple method for site-specific mutagenesis that leaves the rest of the template unaltered / Marjana Tomic-Canic, Ivana Sunjevaric, Miroslav Blumenberg
- Multiple site-directed mutagenesis / Kolari S. Bhat
- Construction of linker-scanning mutations by oligonucleotide ligation / Grace M. Hobson, Patricia P. Harlow, Pamela A. Benfield
- Construction of linker-scanning mutations using PCR / Patricia P. Harlow, Grace M. Hobson, Pamela A. Benfield
- Use of codon cassette mutagenesis for saturation mutagenesis / Deena M. Kegler-Ebo, Glenda W. Polack, Daniel DiMaio
- Saturation mutagenesis by mutagenic oligonucleotide-directed PCR amplification (Mod-PCR) / Lillian W. Chiang
- Random mutagenesis of short target DNA sequences via PCR with degenerate oligonucleotides / Frank Kirchhof, Ronald C. Desrosiers
- Random sequence mutagenesis for the generation of active enzymes / Margaret E. Black, Lawrence A. Loeb
- Random mutagenesis by using mixtures of dNTP and dITP in PCR / Oscar P. Kuipers
- PCR-mediated chemical mutagenesis / Donald J. Roufa
- Oligonucleotide-directed random mutagenesis using the phosphorothioate method / Susan J. Dale, Maxine Belfield
- An efficient random mutagenesis technique using an E. coli mutator strain / Alan Greener, Marie Callahan, Bruce Jerpseth.