Post-transcriptional gene regulation /

Post-transcriptional gene regulation /

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Bibliographic Details
Imprint:Totowa, N.J. : Humana Press, ©2008.
Description:1 online resource (xviii, 318 pages) : illustrations
Language:English
Series:Methods in molecular biology ; 419
Methods in molecular biology (Clifton, N.J.) ; v. 419.
Subject:
Genetic regulation -- Laboratory manuals.
Messenger RNA -- Laboratory manuals.
Cellular control mechanisms -- Laboratory manuals.
Bioinformatics.
Gene Expression Regulation.
Computational Biology.
RNA Processing, Post-Transcriptional.
Bioinformatics.
Cellular control mechanisms.
Genetic regulation.
Messenger RNA.
Laboratory Manual.
Laboratory manuals.
Format: E-Resource Book
URL for this record:http://pi.lib.uchicago.edu/1001/cat/bib/11177467
Hidden Bibliographic Details
Other authors / contributors:Wilusz, Jeffrey.
ISBN:9781597450331
1597450332
1588297837
9781588297839
Digital file characteristics:text file PDF
Notes:Includes bibliographical references and index.
English.
Print version record.
Summary:In Post-Transcriptional Gene Regulation, renowned authors present current technical approaches to most aspects of post-transcriptional control and provide a useful and versatile laboratory bench resource. With chapters split into sections covering bioinformatics, fundamental aspects of the study of RNA biology, and techniques for specific aspects of RNA biology, the expert authors have filled the book with invaluable tricks of the trade, perfected in their state-of-the-art laboratories. This new volume from the Methods in Molecular Biology series is convinently divided into three sections. The first section presents a series of bioinformatic approaches to address the use of RNA databases and algorithms to the study of post-transcriptional regulation involving untranslated regions of transcripts. In the second section, a series of methods applicable to fundamental issues in mRNA biology are presented. These include RNA structure/function, mRNP analysis and novel methods for mRNA labeling and isolation. The third section of this volume presents methodologies to study particular aspects of post-transcriptional control. This section includes methods for the study of alternative splicing and 3' end processing, mRNA localization, mRNA translation, mRNA stability and si/miRNA regulation. Collectively, Post-Transcriptional Gene Regulation provides the reader with a useful and versatile laboratory bench resource that will become an essential reference in the field.
Other form:Print version: Post-transcriptional gene regulation. Totowa, NJ : Humana Press, ©2008 9781588297839
Standard no.:10.1007/978-1-59745-033-1
Table of Contents:
  • Bioinformatics approaches for studying untranslated regions of mRNAs / Paramjeet S. Bagga
  • Identification of mRNA polyadenylation sites in genomes using cDNA sequences, expressed sequence tags, and trace / Ju Youn Lee, Ji Yeon Park, and Bin Tian
  • Bioinformatic tools for studying post-transcriptional gene regulation : the UAlbany TUTR Collection and other informatic resources / Francis Doyle [and others]
  • In-line probing analysis of riboswitches / Elizabeth E. Regulski and Ronald R. Breaker
  • Ribotrap : targeted purification of RNA-specific RNPs from cell lysates through immunoaffinity precipitation to identify regulatory proteins and RNAs / Dale L. Beach and Jack D. Keene
  • Advances in RIP-chip analysis : RNA-binding protein immunoprecipitation-microarray profiling / Timothy E. Baroni [and others]
  • Biosensors for RNA aptamers-protein interaction / Sara Tombelli, Maria Minunni, and Marco Mascini
  • A tethering approach to study proteins that activate mRNA turnover in human cells / Sandra L. Clement and Jens Lykke-Andersen
  • RNA analysis by biosynthetic tagging using a 4-thiouracil and uracil phosphoribosyltransferase / Gusti M. Zeiner [and others]
  • Efficient 5' cap-dependent RNA purification : use in identifying and studying subsets of RNA / Edyta Z. Bajak and Curt H. Hagedorn
  • Enrichment of alternatively spliced isoforms / Julian P. Venables.
  • In vivo methods to assess polyadenylation efficiency / Lisa K. Hague, Tyra Hall-Pogar, and Carol S. Lutz
  • Monitoring the temporal and spatial distribution of RNA in living yeast cells / Roy M. Long and Carl R. Urbinati
  • Analysis of mRNA partitioning between the cytosol and endoplasmic reticulum compartments of mammalian cells / Samuel B. Stephens [and others]
  • In vivo and in vitro analysis of poly(A) length effects on mRNA translation / Jing Peng, Elizabeth L. Murray, and Daniel R. Schoenberg
  • A ribosomal density-mapping procedure to explore ribosome positions along translating mRNAs / Naama Eldad and Yoav Arava
  • Identification of changes in gene expression by quantitation of mRNA levels / Wendy M. Olivas
  • Application of the Invader® RNA assay to the polarity of vertebrate mRNA decay / Elizabeth L. Murray and Daniel R. Schoenberg
  • Development of an in vitro mRNA decay system in insect cells / Kevin Sokoloski, John R. Anderson, and Jeffrey Wilusz
  • Using synthetic precursor and inhibitor miRNAs to understand miRNA function / Lance P. Ford and Angie Cheng
  • A step-by-step procedure to analyze the efficacy of siRNA using real-time PCR / Angie Cheng, Charles L. Johnson, and Lance P. Ford.

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