Reporter genes : a practical guide /

Reporter genes : a practical guide /

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Bibliographic Details
Imprint:	Totowa, N.J. : Humana Press, ©2007.
Description:	1 online resource (x, 158 pages) : illustrations (some color)
Language:	English
Series:	Methods in molecular biology ; v. 411 Methods in molecular biology (Clifton, N.J.) ; v. 411.
Subject:	Reporter genes. Mammals -- Cytology. Mammals -- Genetics. Cytological Techniques. Genes, Reporter. Mammals -- genetics. Mammals -- Cytology. Mammals -- Genetics. Reporter genes.
Format:	E-Resource Book
URL for this record:	http://pi.lib.uchicago.edu/1001/cat/bib/11178965

Hidden Bibliographic Details
Other authors / contributors:	Anson, Donald S.
ISBN:	9781597455497 1597455490 1281136964 9781281136961 9786611136963 6611136967 1603270728 9781603270724 9781588297396 158829739X
Digital file characteristics:	text file PDF
Notes:	Includes bibliographical references and index. English. Print version record.
Summary:	Reporter genes have played, and continue to play, a vital role in many areas of biological research by providing a ready means for qualitative and quantitative assessment of the activity of genes and location of gene products in different environments. In Reporter Genes: A Practical Guide, renowned researchers describe practical protocols for experimentation with the most useful reporter genes for mammalian systems that are available, concentrating on those marker genes that are currently most commonly used. Among the topics covered in this volume are methodologies for staining and visualization of ß-galactosidase in embryos and tissues, immunohistochemical detection of ß-galactosidase, detection of reporter gene expression in murine airways, and three dimensional analysis of molecular signals with episcopic imaging techniques. Also covered in this groundbreaking text are fluorescent proteins, detection of GFP during nervous system development, fluorescent protein reporter systems for single cell measurements, and non-invasive imaging of molecular events. Comprehensive and illuminating, Reporter Genes: A Practical Guide will be an oft-used reference for geneticists, molecular engineers, and molecular biologists.
Other form:	Print version: Reporter genes. Totowa, N.J. : Humana Press, ©2007 9781588297396
Standard no.:	10.1007/978-1-59745-549-7

Description
Summary:	Reporter genes have played, and continue to play, a vital role in many areas of biological research by providing a ready means for qualitative and quantitative assessment of the activity of genes and location of gene products in different environments. This book describes practical protocols for experimentation with the most useful reporter genes for mammalian systems that are available and will concentrate on those marker genes that are currently most commonly used.
Physical Description:	1 online resource (x, 158 pages) : illustrations (some color)
Bibliography:	Includes bibliographical references and index.
ISBN:	9781597455497 1597455490 1281136964 9781281136961 9786611136963 6611136967 1603270728 9781603270724 9781588297396 158829739X