Protein stability and folding : theory and practice /
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Imprint: | Totowa, N.J. : Humana Press, ©1995. |
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Description: | 1 online resource (x, 377 pages) : illustrations |
Language: | English |
Series: | Methods in molecular biology ; v. 40 Methods in molecular biology (Clifton, N.J.) ; v. 40. |
Subject: | |
Format: | E-Resource Book |
URL for this record: | http://pi.lib.uchicago.edu/1001/cat/bib/11179019 |
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072 | 7 | |a SCI007000. |2 bisacsh | |
245 | 0 | 0 | |a Protein stability and folding : |b theory and practice / |c edited by Bret A. Shirley. |
260 | |a Totowa, N.J. : |b Humana Press, |c ©1995. | ||
300 | |a 1 online resource (x, 377 pages) : |b illustrations | ||
336 | |a text |b txt |2 rdacontent | ||
337 | |a computer |b c |2 rdamedia | ||
338 | |a online resource |b cr |2 rdacarrier | ||
490 | 1 | |a Methods in molecular biology ; |v v. 40 | |
504 | |a Includes bibliographical references and index. | ||
505 | 0 | |a Noncovalent forces important to the conformational stability of protein structures / Kenneth P. Murphy -- Degradative covalent reactions important to protein stability / David B. Volkin, Henryk Mach, C. Russell Middaugh -- Fluorescence spectroscopy / Catherine A. Royer -- Ultraviolet absorption spectroscopy / Henryk Mach, David B. Volkin, Carl J. Burke, C. Russell Middaugh -- Circular dichroism / Kunihiro Kuwajima -- Infrared spectroscopy / C. Russell Middaugh, Henryk Mach, James A. Ryan, Gautam Sanyal, David B. Volkin -- Identifying sites of posttranslational modifications in proteins via HPLC peptide mapping / Kenneth R. Williams, Kathryn L. Stone -- Urea and guanidine hydrochloride denaturation curves / Bret A. Shirley -- Differential scanning calorimetry / Ernesto Freire -- Disulfide bonds in protein folding and stability / Nigel Darby, Thomas E. Creighton -- Solvent stabilization of protein structure / Serge N. Timasheff -- Site-directed mutagenesis to study protein folding and stability / Philip N. Bryan -- Hydrogen exchange techniques / J. Martin Scholtz, Andrew D. Robertson -- Protein folding kinetics / Thomas Kiefhaber -- Molten globules / Anthony L. Fink -- Chaperonin-assisted protein folding of the enzyme rhodanese by GroEL/GroES / Paul M. Horowitz. | |
588 | 0 | |a Print version record. | |
520 | |a In Protein Stability and Folding: Theory and Practice, world-class scientists present in a single volume a comprehensive selection of hands-on recipes for all of the major techniques needed to understand the conformational stability of proteins, as well as their three-dimensional folding. The distinguished contributors provide clear, step-by-step instructions along with many troubleshooting tips, alternative procedures, and informative explanations about why certain steps are necessary. Even highly skilled researchers will find many time-saving methods. Among the techniques discussed are fluorescent, ultraviolet, and infrared spectroscopy; HPLC peptide mapping; differential scanning calorimetry; and hydrogen exchange. Shirley's Protein Stability and Folding: Theory and Practice will ensure a significant difference in the outcome of your experiments, producing the result desired even for beginners. | ||
546 | |a English. | ||
650 | 0 | |a Protein folding. |0 http://id.loc.gov/authorities/subjects/sh89006334 | |
650 | 0 | |a Proteins |x Conformation. |0 http://id.loc.gov/authorities/subjects/sh89006335 | |
650 | 1 | 2 | |a Protein Conformation |
650 | 2 | 2 | |a Protein Folding |
650 | 6 | |a Protéines |x Repliement. | |
650 | 6 | |a Protéines |x Conformation. | |
650 | 7 | |a Protein folding |2 fast | |
650 | 7 | |a Proteins |x Conformation |2 fast | |
653 | 0 | 0 | |a biochemie |
653 | 0 | 0 | |a biochemistry |
653 | 0 | 0 | |a levenswetenschappen |
653 | 0 | 0 | |a life sciences |
653 | 1 | 0 | |a Biology (General) |
653 | 1 | 0 | |a Biologie (algemeen) |
655 | 2 | |a Laboratory Manual |0 https://id.nlm.nih.gov/mesh/D020484 | |
655 | 4 | |a Fulltext. | |
655 | 4 | |a Internet Resource. | |
655 | 7 | |a Laboratory manuals. |2 lcgft |0 http://id.loc.gov/authorities/genreForms/gf2014026120 | |
655 | 7 | |a Manuels de laboratoire. |2 rvmgf | |
700 | 1 | |a Shirley, Bret A. |0 http://id.loc.gov/authorities/names/n94105606 | |
776 | 0 | 8 | |i Print version: |t Protein stability and folding. |d Totowa, N.J. : Humana Press, 1995 |z 0896033015 |w (DLC) 94039996 |w (OCoLC)31517088 |
830 | 0 | |a Methods in molecular biology (Clifton, N.J.) ; |v v. 40. | |
856 | 4 | 0 | |u https://link.springer.com/10.1385/0896033015 |y Springer Nature |
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