Protein stability and folding : theory and practice /

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Bibliographic Details
Imprint:Totowa, N.J. : Humana Press, ©1995.
Description:1 online resource (x, 377 pages) : illustrations
Language:English
Series:Methods in molecular biology ; v. 40
Methods in molecular biology (Clifton, N.J.) ; v. 40.
Subject:
Format: E-Resource Book
URL for this record:http://pi.lib.uchicago.edu/1001/cat/bib/11179019
Hidden Bibliographic Details
Other authors / contributors:Shirley, Bret A.
ISBN:9781592595273
1592595278
1280836490
9781280836497
9786610836499
6610836493
0896033015
9780896033016
Notes:Includes bibliographical references and index.
English.
Print version record.
Summary:In Protein Stability and Folding: Theory and Practice, world-class scientists present in a single volume a comprehensive selection of hands-on recipes for all of the major techniques needed to understand the conformational stability of proteins, as well as their three-dimensional folding. The distinguished contributors provide clear, step-by-step instructions along with many troubleshooting tips, alternative procedures, and informative explanations about why certain steps are necessary. Even highly skilled researchers will find many time-saving methods. Among the techniques discussed are fluorescent, ultraviolet, and infrared spectroscopy; HPLC peptide mapping; differential scanning calorimetry; and hydrogen exchange. Shirley's Protein Stability and Folding: Theory and Practice will ensure a significant difference in the outcome of your experiments, producing the result desired even for beginners.
Other form:Print version: Protein stability and folding. Totowa, N.J. : Humana Press, 1995 0896033015
Standard no.:10.1385/0896033015.

MARC

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245 0 0 |a Protein stability and folding :  |b theory and practice /  |c edited by Bret A. Shirley. 
260 |a Totowa, N.J. :  |b Humana Press,  |c ©1995. 
300 |a 1 online resource (x, 377 pages) :  |b illustrations 
336 |a text  |b txt  |2 rdacontent 
337 |a computer  |b c  |2 rdamedia 
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490 1 |a Methods in molecular biology ;  |v v. 40 
504 |a Includes bibliographical references and index. 
505 0 |a Noncovalent forces important to the conformational stability of protein structures / Kenneth P. Murphy -- Degradative covalent reactions important to protein stability / David B. Volkin, Henryk Mach, C. Russell Middaugh -- Fluorescence spectroscopy / Catherine A. Royer -- Ultraviolet absorption spectroscopy / Henryk Mach, David B. Volkin, Carl J. Burke, C. Russell Middaugh -- Circular dichroism / Kunihiro Kuwajima -- Infrared spectroscopy / C. Russell Middaugh, Henryk Mach, James A. Ryan, Gautam Sanyal, David B. Volkin -- Identifying sites of posttranslational modifications in proteins via HPLC peptide mapping / Kenneth R. Williams, Kathryn L. Stone -- Urea and guanidine hydrochloride denaturation curves / Bret A. Shirley -- Differential scanning calorimetry / Ernesto Freire -- Disulfide bonds in protein folding and stability / Nigel Darby, Thomas E. Creighton -- Solvent stabilization of protein structure / Serge N. Timasheff -- Site-directed mutagenesis to study protein folding and stability / Philip N. Bryan -- Hydrogen exchange techniques / J. Martin Scholtz, Andrew D. Robertson -- Protein folding kinetics / Thomas Kiefhaber -- Molten globules / Anthony L. Fink -- Chaperonin-assisted protein folding of the enzyme rhodanese by GroEL/GroES / Paul M. Horowitz. 
588 0 |a Print version record. 
520 |a In Protein Stability and Folding: Theory and Practice, world-class scientists present in a single volume a comprehensive selection of hands-on recipes for all of the major techniques needed to understand the conformational stability of proteins, as well as their three-dimensional folding. The distinguished contributors provide clear, step-by-step instructions along with many troubleshooting tips, alternative procedures, and informative explanations about why certain steps are necessary. Even highly skilled researchers will find many time-saving methods. Among the techniques discussed are fluorescent, ultraviolet, and infrared spectroscopy; HPLC peptide mapping; differential scanning calorimetry; and hydrogen exchange. Shirley's Protein Stability and Folding: Theory and Practice will ensure a significant difference in the outcome of your experiments, producing the result desired even for beginners. 
546 |a English. 
650 0 |a Protein folding.  |0 http://id.loc.gov/authorities/subjects/sh89006334 
650 0 |a Proteins  |x Conformation.  |0 http://id.loc.gov/authorities/subjects/sh89006335 
650 1 2 |a Protein Conformation 
650 2 2 |a Protein Folding 
650 6 |a Protéines  |x Repliement. 
650 6 |a Protéines  |x Conformation. 
650 7 |a Protein folding  |2 fast 
650 7 |a Proteins  |x Conformation  |2 fast 
653 0 0 |a biochemie 
653 0 0 |a biochemistry 
653 0 0 |a levenswetenschappen 
653 0 0 |a life sciences 
653 1 0 |a Biology (General) 
653 1 0 |a Biologie (algemeen) 
655 2 |a Laboratory Manual  |0 https://id.nlm.nih.gov/mesh/D020484 
655 4 |a Fulltext. 
655 4 |a Internet Resource. 
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655 7 |a Manuels de laboratoire.  |2 rvmgf 
700 1 |a Shirley, Bret A.  |0 http://id.loc.gov/authorities/names/n94105606 
776 0 8 |i Print version:  |t Protein stability and folding.  |d Totowa, N.J. : Humana Press, 1995  |z 0896033015  |w (DLC) 94039996  |w (OCoLC)31517088 
830 0 |a Methods in molecular biology (Clifton, N.J.) ;  |v v. 40. 
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