Plant meiosis : methods and protocols /

Saved in:

Bibliographic Details
Imprint:	New York : Humana Press, ©2013.
Description:	1 online resource (xv, 238 pages) : illustrations (some color).
Language:	English
Series:	Methods in molecular biology, 1940-6029 ; 990 Methods in molecular biology (Clifton, N.J.) ; 990.
Subject:	Plant cells and tissues -- Laboratory manuals. Meiosis -- Laboratory manuals. Meiosis -- Laboratory Manuals. Plant Cells -- Laboratory Manuals. Meiosis. Plant cells and tissues. Laboratory manuals.
Format:	E-Resource Book
URL for this record:	http://pi.lib.uchicago.edu/1001/cat/bib/11181827

Hidden Bibliographic Details
Other authors / contributors:	Pawlowski, Wojciech P. Grelon, Mathilde. Armstrong, Susan (University lecturer)
ISBN:	9781627033336 1627033335 1627033327 9781627033329 9781627033329
Digital file characteristics:	text file PDF
Notes:	Includes bibliographical references and index. English.
Summary:	Meiosis is one of the most critical processes in eukaryotes, required for continuation of species and generation of new variation. In plants, meiotic recombination is by far the most important source of genetic variation. In Plant Meiosis: Methods and Protocols, expert researchers in the field detail methods for molecular cytogenetics and chromosome analysis in plants. These state-of -the-art protocols allow studying the organization and behavior of the genetic material in a wide range of both model and crop species. Written in the highly successful Methods in Molecular Biologyseries format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Plant Meiosis: Methods and Protocols provides and extensive list of protocols developed and used in a number of laboratories at the cutting edge of meiosis and chromosome research.
Other form:	Printed edition: 9781627033329
Standard no.:	10.1007/978-1-62703-333-6

Table of Contents:

Spreading and fluorescence in situ hybridization of male and female meiocyte chromosomes from Arabidopsis thaliana for cytogenetical analysis / Susan Armstrong
Analysis of plant meiotic chromosomes by chromosome painting / Martin A. Lysak and Terezie Mandáková
Using sequential fluorescence and genomic in situ hybridization (FISH and GISH) to distinguish the A and C genomes in Brassica napus / Elaine C. Howell and Susan Armstrong
Labeling meiotic chromosomes in maize with fluorescence in situ hybridization / Zhi Gao [and others]
Examining female meiocytes of maize by confocal microscopy / Philippa Barrell and Ueli Grossniklaus
Three-dimensional acrylamide fluorescence in situ hybridization for plant cells / Elizabeth S. Howe, Shaun P. Murphy, and Hank W. Bass
Analyzing maize meiotic chromosomes with super-resolution structured illumination microscopy / Chung-Ju Rachel Wang
Live imaging of chromosome dynamics / Moira J. Sheehan, R. Kelly Dawe, and Wojciech P. Pawlowski
Immunolocalization of meiotic proteins in brassicaceae : method 1 / Liudmila A. Chelysheva, Laurie Grandont, and Mathilde Grelon
Immunolocalization of meiotic proteins in Arabidopsis thaliana : method 2 / Susan Armstrong and Kim Osman
Immunolocalization protocols for visualizing meiotic proteins in Arabidopsis thaliana : method 3 / Xiaohui Yang, Li Yuan, and Christopher A. Makaroff
Time course for the analysis of meiotic progression in Arabidopsis thaliana / Susan Armstrong
Analyzing meiotic chromosomes in rice / Zhukuan Cheng
Analyzing meiosis in barley / James D. Higgins
Preparing SC spreads with RNs for EM analysis / Lorinda K. Anderson and Stephen M. Stack
Analysis of the synaptonemal complex in brassica using TEM / Susan Armstrong
Preparing thin sections of meiotic nuclei for transmission electron microscopy / Ljudmilla Timofejeva
Characterization of meiotic non-crossover molecules from Arabidopsis thaliana Pollen / Hossein Khademian [and others]
Chromatin immunoprecipitation for studying chromosomal localization of meiotic proteins in maize / Yan He, Gaganpreet Sidhu, and Wojciech P. Pawlowski
Analyzing the meiotic transcriptome using isolated meiocytes of Arabidopsis thaliana / Changbin Chen and Ernest F. Retzel
Analysis of Meiotic Protein Complexes from arabidopsis and brassica using affinity-based proteomics / Kim Osman [and others]
Identifying meiotic mutants in Arabidopsis thaliana / Wayne Crismani and Raphaël Mercier.

Plant meiosis : methods and protocols /

Similar Items