Molecular embryology : methods and protocols /

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Bibliographic Details
Edition:	2nd ed.
Imprint:	Totowa, NJ : Humana Press, ©2008.
Description:	1 online resource (xvii, 798 pages, 12 unnumbered pages of plates) : illustrations (some color)
Language:	English
Series:	Methods in molecular biology ; v. 461 Methods in molecular biology (Clifton, N.J.) ; v. 461.
Subject:	Vertebrates -- Embryology -- Methodology. Chemical embryology -- Methodology. Embryology -- methods. Vertebrates -- embryology.
Format:	E-Resource Book
URL for this record:	http://pi.lib.uchicago.edu/1001/cat/bib/11186039

Hidden Bibliographic Details
Other authors / contributors:	Sharpe, Paul T. Mason, Ivor.
ISBN:	9781603274838 1603274839 9781588294319 1588294315
Notes:	Includes bibliographical references and index. Print version record.
Summary:	This volume provides a guide to methods used across the growing field of vertebrate molecular embryology. Time-tested techniques take advantage of the most commonly used vertebrate experimental models: murine embryos for their genetics, chick embryos for in vivo manipulation, zebrafish for mutagenesis, amphibian embryos, and nonvertebrate chordates. This edition collects classic protocols which have become standard techniques in the laboratory and presents them in a complementary fashion with novel and emerging approaches, allowing researcher to become more familiar with commonly studied embryos used in biomedical research.
Other form:	Print version: Molecular embryology. 2nd ed. New York, NY : Humana Press, ©2008 9781588294319

Table of Contents:

The mouse as a developmental model / Paul T. Sharpe
Culture of postimplantation mouse embryos / Paul Martin and David L. Cockroft
Organ culture in the analysis of tissue interactions / Irma Thesleff and Carin Sahlberg
Treatment of mice with retinoids in vivo and in vitro / Gillian M. Morriss-Kay
Analysis of skeletal ontogenesis through differential staining of bone and cartilage / Michael J. Depew
Cell grafting and labeling in postimplantation mouse embryos / Gabriel A. Quinlan [and others]
Production of transgenic rodents by the microinjection of cloned DNA into fertilized one-celled eggs / David Murphy
Cre recombinase mediated alterations of the mouse genome using embryonic stem cells / Anna-Katerina Hadjantonakis, Melinda Pirity, and András Nagy
Gene trapping in mouse embryonic stem cells / Jane Brennan and William C. Skarnes
Application of lacZ transgenic mice to cell lineage studies / Catherine M. Watson [and others]
Transgenic RNA interference to investigate gene function in the mouse / Tilo Kunath
Mouse primordial germ cells : isolation and in vitro culture / Patricia A. Labosky and Brigid L.M. Hogan
Gene transfer to the rodent embryo by retroviral vectors / Marla B. Luskin
The avian embryo : an overview / Ivor Mason
Chick embryos : incubation and isolation / Ivor Mason
New culture / Amata Hornbruch
EC culture : a method to culture early chick embryos / Andrea Streit
Grafting Henson's node / Claudio D. Stern
Microsurgical manipulation of the notochord / Lúcia E. Alvares [and others]
Transplantation of avian neural tissue / Sarah Guthrie
Grafting of apical ridge and polarizing region / Cheryl Tickle
Tissue recombinations in collagen gels / Marysia Placzek
Quail-chick chimeras / Marie-Aimée Teillet, Catherine Ziller, and Nicole M. Le Douarin
Using fluorescent dyes for fate mapping, lineage analysis, and axon tracing in the chick embryo / Jonathan D.W. Clarke
Gene transfer in avian embryos using replication-competent retroviruses / Cairine Logan and Philippa Francis-West
Electroporation in avian embryos / Jun-ichi Funahashi and Harukazu Nakamura
An overview of Xenopus development / Michael Jones and James C. Smith
Mesoderm induction assays / C. Michael Jones and James C. Smith
Experimental embryological methods for analysis of neural induction in the amphibian / Ray Keller, Ann Poznanski, and Tamira Elul
A method for generating transgenic frog embryos / Shoko Ishibashi, Kristin L. Kroll, and Enrique Amaya
Axolotl/newt / Malcolm Maden
The zebrafish : an overview of its early development / Nigel Holder and Qiling Xu
Small-scale marker-based screening for mutations in zebrafish development / Peter D. Currie, Thomas F. Schilling, and Philip W. ingham
Microinjection and cell transplantation in zebrafish embryos / Qiling Xu, Derek Stemple, and Katherine Joubin
Recent advances in meganuclease- and transposon-mediated transgenesis of medaka and zebrafish / Clemens Grabher and Joachim Wittbrodt
Retinoids in nonmammalian embryos / Malcolm Maden
Protochordates / Peter W.H. Holland and Hiroshi Wada
Subtractive hybridization and construction of cDNA libraries / Bruce Blumberg and Juan Carlos Izpisúa Belmonte
Differential display of eukaryotic mRNA / Antonio Tugores and Juan Carlos Izpisúa Belmonte
Using DNA microarrays / Clare Pritchard, Peter Underhill, and Andy Greenfield
Profiling gene transcription in the developing embryo : microarray analysis on gene chips / David Chambers and Andrew Lumsden
RT-PCR on embryos using degenerate oligonucleotide primers / Anthony Graham
Single-cell RT-PCR cDNA subraction / Ebrahim Sakhinia [and others]
In-situ hybridization of rdioactive riboprobes to RNA in tissue sections / Radma Mahmood and Ivor Mason
In-situ hybridization and immunohistochemistry in whole embryos / Carol Irving
Wholemount in situ hybridization to Xenopus embryos / C. Michael Jones and James C. Smith
Wholemount in situ hybridization to Amphioxus embryos / Peter W.H. Holland
In-situ hybridization to sections (nonradioactive) / Maria Rex and Paul J. Scotting
Immunohistochemistry using polyester wax / Andrew Kent
Immunohistochemistry on whole embryos / Ivor Mason
Whole embryo assays for programmed cell death / Anthony Graham
Protein techniques : immunoprecipitation, in vitro kinase assays, and western blotting / Clive Dickson
Microscopy and photmicrography techniques / Richard J.T. Wingate.

Molecular embryology : methods and protocols /

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