RNA-protein interaction protocols.
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| Edition: | 2nd ed. / edited by Ren-Jang Lin. |
|---|---|
| Imprint: | Totowa, N.J. : Humana ; London : Springer [distributor], 2008. |
| Description: | 1 online resource (xv, 416 pages) : illustrations |
| Language: | English |
| Series: | Methods in molecular biology ; v. 488 Methods in molecular biology (Clifton, N.J.) ; v. 488. |
| Subject: | |
| Format: | E-Resource Book |
| URL for this record: | http://pi.lib.uchicago.edu/1001/cat/bib/11186069 |
MARC
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| 245 | 0 | 0 | |a RNA-protein interaction protocols. |
| 250 | |a 2nd ed. / |b edited by Ren-Jang Lin. | ||
| 260 | |a Totowa, N.J. : |b Humana ; |a London : |b Springer [distributor], |c 2008. | ||
| 300 | |a 1 online resource (xv, 416 pages) : |b illustrations | ||
| 336 | |a text |b txt |2 rdacontent | ||
| 337 | |a computer |b c |2 rdamedia | ||
| 338 | |a online resource |b cr |2 rdacarrier | ||
| 490 | 1 | |a Methods in molecular biology ; |v v. 488 | |
| 504 | |a Includes bibliographical references and index. | ||
| 505 | 0 | |a 1. Isolation of a sequence-specific RNA binding protein, polypyrimidine tract binding protein using RNA affinity chromatography / Shalini Sharma -- 2. An affinity oligonucleotide displacement strategy to purify ribonucleoprotein complexes applied to human telomerase /Isabel Kurth, Gaël Cristofari, Joachim Lingner -- 3. RNA affinity tags for the rapid purification and investigation of RNAs and RNA-protein complexes / Scott C. Walker [and others] -- 4. Assembly and glycerol gradient isolation of yeast spliceosomes containing transcribed or synthetic U6 SnRNA / Ken Dery, Shyue-Lee Yean, Ren-Jang Lin -- 5. Purification of ribonucleoproteins using peptide-elutable antibodies and other affinity techniques / Scott Stevens -- 6. CLIP: cross-linking and immunoprecipitation of in vivo RNA targets of RNA binding proteins / Kirk B Jensen, Robert B. Darnell -- 7. Quantitative analysis of protein-RNA interactions by gel mobility shift / Sean P. Ryder, Michael I. Recht, James R. Williamson -- 8. Monitoring assembly of ribonucleoprotein complexes by isothermal titration calorimetry / Michael I. Recht, Sean P. Ryder, James R. Williamson -- 9. Characterization of RNA-protein interactions by phosphorothioate footprinting and its applications to the ribosome / A. Ozlem Tastan Bishop [and others] -- 10. In vivo analysis of ribonucleoprotein complexes using nucleotide analog interference mapping / Lara Weinstein Szewczak -- 11. T7 RNA polymerase mediated incorporation of 8-N3AMP into RNA for studying protein-RNA interactions / Rajesh Gaur -- 12. A simple crosslinking method, CLAMP, to map the sites of RNA contacting domains within a protein / Hiren Banerjee, Ravinder Singh -- 13. Proteins specifically modified with a chemical nuclease as probes of RNA-protein interaction / Oliver A. Ken, Andrew M. MacMillan -- 14. RNA-protein crosslink mapping using TEV protease / Ian A. Turner [and others] -- 15 Structural analysis of protein-RNA interactions with mass spectrometry / Mamuka Kvaratskhelia, Stuart F.J. Le Grice -- 16. Analysing RNA-protein cross-linking sites in unlabeled ribonucleoprotein complexes by mass spectrometry / Henning Urlaub, Eva Kühn-Hölsken, Reinhard Lührmann. | |
| 505 | 0 | |a 17. In vitro selection of random RNA fragments to identify protein binding sites within large RNAs / Ulrich Stelzl, Knud H. Nierhaus -- 18. Immunoprecipitation analysis to study RNA-protein interactions in xenopus oocytes / Naoto Mabuchi, Kaoru Masuyama, Mutsuhito Ohno -- 19. Mapping the regions of RNase P catalytic RNA that are potentially in close contact with its protein cofactor / Phong Trang, Fenyong Liu -- 20. Quantification of microRNAs, splicing isoforms, and homologous mRNAs with the invader assay / Peggy S. Eis, Mariano Garcia-Blanco -- 21. Analysis of RNA structure and RNA-protein interactions in mammalian cells by use of terminal transferase-dependent PCR / Hsiu-Hua Chen, Jeanne LeBon, Arthur D. Riggs -- 22. Duplex unwinding and RNP remodeling with RNA helicases / Eckhard Jankowsky, Margaret E. Fairman -- 23. Preparation of efficient splicing extracts from whole cells, nuclei, and cytoplasmic fractions / Naoyuki Kataoka, Gideon Dreyfuss -- 24. Designing and utilization of siRNAs targeting RNA-binding proteins / Dong-Ho Kim, Mark Behlke, John J. Rossi -- 25. The use of saccharomyces cerevisiae proteomic libraries to identify RNA-modifying proteins / Jane E. Jackman, Eric M. Phizicky. | |
| 588 | 0 | |a Print version record. | |
| 520 | 3 | |a This book provides a collection of techniques developed or refined with tried-and-true methods. The contributors explore the isolation and characterization of RNA-protein complexes, the analysis and measurement of RNA-protein interaction, and related novel techniques and strategies. | |
| 546 | |a English. | ||
| 650 | 0 | |a RNA-protein interactions |v Laboratory manuals. | |
| 650 | 2 | |a RNA-Binding Proteins |x analysis. | |
| 650 | 2 | |a RNA |x chemistry. | |
| 650 | 2 | |a Ribonucleoproteins |x analysis. | |
| 650 | 7 | |a RNA-protein interactions. |2 fast |0 (OCoLC)fst01086260 | |
| 653 | 0 | 0 | |a rna |
| 653 | 0 | 0 | |a eiwitten |
| 653 | 0 | 0 | |a proteins |
| 653 | 0 | 0 | |a bindende eiwitten |
| 653 | 0 | 0 | |a binding proteins |
| 653 | 0 | 0 | |a biochemie |
| 653 | 0 | 0 | |a biochemistry |
| 653 | 0 | 0 | |a chromatografie |
| 653 | 0 | 0 | |a chromatography |
| 653 | 0 | 0 | |a moleculaire interacties |
| 653 | 0 | 0 | |a molecular interactions |
| 653 | 0 | 0 | |a protocollen |
| 653 | 0 | 0 | |a protocols |
| 653 | 1 | 0 | |a Molecular Biology (General) |
| 653 | 1 | 0 | |a Moleculaire biologie (algemeen) |
| 655 | 4 | |a Electronic books. | |
| 655 | 7 | |a Laboratory manuals. |2 fast |0 (OCoLC)fst01920776 | |
| 700 | 1 | |a Lin, Ren-Jang. |0 http://id.loc.gov/authorities/names/n2008184626 | |
| 776 | 0 | 8 | |i Print version: |t RNA-protein interaction protocols. |b 2nd ed. |d Totowa, N.J. : Humana ; London : Springer [distributor], 2008 |z 9781588294197 |w (DLC) 2008921785 |w (OCoLC)166378576 |
| 830 | 0 | |a Methods in molecular biology (Clifton, N.J.) ; |v v. 488. | |
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| 928 | |t Library of Congress classification |a QP623.8.P7 |l Online |c UC-FullText |u https://link.springer.com/10.1007/978-1-60327-475-3 |z Springer Nature |g ebooks |i 12531202 | ||