Transgenic wheat, barley and oats : production and characterization protocols /
Saved in:
Imprint: | New York, NY : Humana Press, ©2009. |
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Description: | 1 online resource (x, 349 pages, 8 unnumbered pages of plates) : illustrations (some color) |
Language: | English |
Series: | Methods in molecular biology, 1940-6029 ; 478 Springer protocols Methods in molecular biology (Clifton, N.J.) ; v. 478. Springer protocols (Series) |
Subject: | |
Format: | E-Resource Book |
URL for this record: | http://pi.lib.uchicago.edu/1001/cat/bib/11186086 |
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245 | 0 | 0 | |a Transgenic wheat, barley and oats : |b production and characterization protocols / |c edited by Huw D. Jones and Peter R. Shewry. |
260 | |a New York, NY : |b Humana Press, |c ©2009. | ||
300 | |a 1 online resource (x, 349 pages, 8 unnumbered pages of plates) : |b illustrations (some color) | ||
336 | |a text |b txt |2 rdacontent | ||
337 | |a computer |b c |2 rdamedia | ||
338 | |a online resource |b cr |2 rdacarrier | ||
347 | |a text file |b PDF |2 rda | ||
490 | 1 | |a Methods in molecular biology, |x 1940-6029 ; |v 478 | |
490 | 1 | |a Springer protocols | |
504 | |a Includes bibliographical references and index. | ||
505 | 0 | |a Transgenic wheat, barley and oats : production and characterization / Paul A. Lazzeri and Huw D. Jones -- Selection of transformed plants / Huw D. Jones and Caroline A. Sparks -- Reporter genes / Alison Huttly -- Biolistics transformation of wheat / Caroline A. Sparks and Huw D. Jones -- Agrobacterium-mediated transformation of bread and durum wheat using freshly isolated immature embryos / Huixia Wu, Angela Doherty and Huw D. Jones -- Floral transformation of wheat / Sujata Agarwal [and others] -- Highly efficient agrobacterium-mediated transformation of wheat via in planta inoculation / Thierry Risacher [and others] -- Barley transformation using biolistic techniques / Wendy A. Harwood and Mark A. Smedley -- Barley transformation using agrobacterium-mediated techniques / Wendy A. Harwood [and others] -- Transformation of oats and its application to improving osmotic stress tolerance / Shahina B. Maqbool [and others] -- Promoter sequences for defining transgene expression / Huw D. Jones and Caroline A. Sparks -- Down-regulation of gene expression by RNA-induced gene silencing / Silvia Travella and Beat Keller -- Gene insertion patterns and sites / Philippe Vain and Vera Thole -- Fluorescent in situ hybridization to detect transgene integration into plant genomes / Trude Schwarzacher -- Establishing substantial equivalence : transcriptomics / María Marcela Baudo [and others] -- Establishing substantial equivalence : proteomics / Alison Lovegrove, Louise Salt, and Peter R. Shewry -- Establishing substantial equivalence : metabolomics / Michael H. Beale, Jane L. Ward, and John M. Baker -- Design and management of field trials of transgenic cereals / Zoltán Bedő, Mariann Rakszegi, and László Láng -- GM risk assessment / Penny A.C. Sparrow -- Transgenic wheat, barley and oats : future prospects / Jim M. Dunwell. | |
588 | 0 | |a Print version record. | |
520 | |a The understanding of the physical and genetic structure of plant genomes and the determination of phenotypes via interactions of coding and non-coding regions with the environment has quickly become the key to the future of plant breeding and agriculture. In Transgenic Wheat, Barley and Oats: Production and Characterization Protocols, expert investigators contribute the latest protocols for the transformation, regeneration and selection of these three species, using both biolistics and Agrobacterium tumefaciens, in order to fill the need for techniques and resources in this vital area of research. Covering such topics as in vitro Agrobacterium co-cultivation, the manipulation of gene expression, and GM risk assessment, this versatile collection focuses on the study of temperate small grain cereals while many of its readily reproducible procedures could be easily adapted to accommodate other cereals and plants. The in-depth chapters adhere to the highly successful Methods in Molecular Biology series format, featuring brief introductions to the subject, lists of the necessary materials and reagents, step-by-step laboratory protocols and tips on troubleshooting and avoiding known pitfalls. Comprehensive and cutting-edge, Transgenic Wheat, Barley and Oats: Production and Characterization Protocols is an ideal guide for scientists pursuing this powerful, forward-thinking genetic strategy. | ||
546 | |a English. | ||
650 | 0 | |a Transgenic plants. |0 http://id.loc.gov/authorities/subjects/sh90003137 | |
650 | 0 | |a Wheat |x Genetics. |0 http://id.loc.gov/authorities/subjects/sh85146370 | |
650 | 0 | |a Barley |x Genetics. |0 http://id.loc.gov/authorities/subjects/sh85011878 | |
650 | 0 | |a Oats |x Genetics. |0 http://id.loc.gov/authorities/subjects/sh85093609 | |
650 | 2 | |a Plants, Genetically Modified. | |
650 | 2 | |a Genetic Engineering |x methods. | |
650 | 2 | |a Triticum |x genetics. | |
650 | 2 | |a Hordeum |x genetics. | |
650 | 2 | |a Avena |x genetics. | |
650 | 7 | |a Barley |x Genetics. |2 fast |0 (OCoLC)fst00827619 | |
650 | 7 | |a Oats |x Genetics. |2 fast |0 (OCoLC)fst01042674 | |
650 | 7 | |a Transgenic plants. |2 fast |0 (OCoLC)fst01154677 | |
650 | 7 | |a Wheat |x Genetics. |2 fast |0 (OCoLC)fst01174357 | |
655 | 2 | |a Laboratory Manual. | |
655 | 4 | |a Electronic books. | |
700 | 1 | |a Jones, Huw D. |0 http://id.loc.gov/authorities/names/nb2009001030 | |
700 | 1 | |a Shewry, P. R. |q (Peter R.) |0 http://id.loc.gov/authorities/names/n92119531 | |
776 | 0 | 8 | |i Print version: |t Transgenic wheat, barley and oats. |d New York, NY : Humana Press, ©2009 |z 9781588299611 |w (DLC) 2008933591 |w (OCoLC)227922736 |
830 | 0 | |a Methods in molecular biology (Clifton, N.J.) ; |v v. 478. | |
830 | 0 | |a Springer protocols (Series) | |
903 | |a HeVa | ||
929 | |a oclccm | ||
999 | f | f | |i 059791cd-6d3c-5718-a9da-fdab334b29f5 |s 506e4b8a-fc3f-50fc-9999-8153554d49c9 |
928 | |t Library of Congress classification |a SB123.57 |l Online |c UC-FullText |u https://link.springer.com/10.1007/978-1-59745-379-0 |z Springer Nature |g ebooks |i 12531195 |