Transgenic wheat, barley and oats : production and characterization protocols /

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Bibliographic Details
Imprint:New York, NY : Humana Press, ©2009.
Description:1 online resource (x, 349 pages, 8 unnumbered pages of plates) : illustrations (some color)
Language:English
Series:Methods in molecular biology, 1940-6029 ; 478
Springer protocols
Methods in molecular biology (Clifton, N.J.) ; v. 478.
Springer protocols (Series)
Subject:
Format: E-Resource Book
URL for this record:http://pi.lib.uchicago.edu/1001/cat/bib/11186086
Hidden Bibliographic Details
Other authors / contributors:Jones, Huw D.
Shewry, P. R. (Peter R.)
ISBN:9781597453790
159745379X
9781588299611
1588299619
Digital file characteristics:text file PDF
Notes:Includes bibliographical references and index.
English.
Print version record.
Summary:The understanding of the physical and genetic structure of plant genomes and the determination of phenotypes via interactions of coding and non-coding regions with the environment has quickly become the key to the future of plant breeding and agriculture. In Transgenic Wheat, Barley and Oats: Production and Characterization Protocols, expert investigators contribute the latest protocols for the transformation, regeneration and selection of these three species, using both biolistics and Agrobacterium tumefaciens, in order to fill the need for techniques and resources in this vital area of research. Covering such topics as in vitro Agrobacterium co-cultivation, the manipulation of gene expression, and GM risk assessment, this versatile collection focuses on the study of temperate small grain cereals while many of its readily reproducible procedures could be easily adapted to accommodate other cereals and plants. The in-depth chapters adhere to the highly successful Methods in Molecular Biology series format, featuring brief introductions to the subject, lists of the necessary materials and reagents, step-by-step laboratory protocols and tips on troubleshooting and avoiding known pitfalls. Comprehensive and cutting-edge, Transgenic Wheat, Barley and Oats: Production and Characterization Protocols is an ideal guide for scientists pursuing this powerful, forward-thinking genetic strategy.
Other form:Print version: Transgenic wheat, barley and oats. New York, NY : Humana Press, ©2009 9781588299611
Standard no.:10.1007/978-1-59745-379-0

MARC

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245 0 0 |a Transgenic wheat, barley and oats :  |b production and characterization protocols /  |c edited by Huw D. Jones and Peter R. Shewry. 
260 |a New York, NY :  |b Humana Press,  |c ©2009. 
300 |a 1 online resource (x, 349 pages, 8 unnumbered pages of plates) :  |b illustrations (some color) 
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490 1 |a Methods in molecular biology,  |x 1940-6029 ;  |v 478 
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504 |a Includes bibliographical references and index. 
505 0 |a Transgenic wheat, barley and oats : production and characterization / Paul A. Lazzeri and Huw D. Jones -- Selection of transformed plants / Huw D. Jones and Caroline A. Sparks -- Reporter genes / Alison Huttly -- Biolistics transformation of wheat / Caroline A. Sparks and Huw D. Jones -- Agrobacterium-mediated transformation of bread and durum wheat using freshly isolated immature embryos / Huixia Wu, Angela Doherty and Huw D. Jones -- Floral transformation of wheat / Sujata Agarwal [and others] -- Highly efficient agrobacterium-mediated transformation of wheat via in planta inoculation / Thierry Risacher [and others] -- Barley transformation using biolistic techniques / Wendy A. Harwood and Mark A. Smedley -- Barley transformation using agrobacterium-mediated techniques / Wendy A. Harwood [and others] -- Transformation of oats and its application to improving osmotic stress tolerance / Shahina B. Maqbool [and others] -- Promoter sequences for defining transgene expression / Huw D. Jones and Caroline A. Sparks -- Down-regulation of gene expression by RNA-induced gene silencing / Silvia Travella and Beat Keller -- Gene insertion patterns and sites / Philippe Vain and Vera Thole -- Fluorescent in situ hybridization to detect transgene integration into plant genomes / Trude Schwarzacher -- Establishing substantial equivalence : transcriptomics / María Marcela Baudo [and others] -- Establishing substantial equivalence : proteomics / Alison Lovegrove, Louise Salt, and Peter R. Shewry -- Establishing substantial equivalence : metabolomics / Michael H. Beale, Jane L. Ward, and John M. Baker -- Design and management of field trials of transgenic cereals / Zoltán Bedő, Mariann Rakszegi, and László Láng -- GM risk assessment / Penny A.C. Sparrow -- Transgenic wheat, barley and oats : future prospects / Jim M. Dunwell. 
588 0 |a Print version record. 
520 |a The understanding of the physical and genetic structure of plant genomes and the determination of phenotypes via interactions of coding and non-coding regions with the environment has quickly become the key to the future of plant breeding and agriculture. In Transgenic Wheat, Barley and Oats: Production and Characterization Protocols, expert investigators contribute the latest protocols for the transformation, regeneration and selection of these three species, using both biolistics and Agrobacterium tumefaciens, in order to fill the need for techniques and resources in this vital area of research. Covering such topics as in vitro Agrobacterium co-cultivation, the manipulation of gene expression, and GM risk assessment, this versatile collection focuses on the study of temperate small grain cereals while many of its readily reproducible procedures could be easily adapted to accommodate other cereals and plants. The in-depth chapters adhere to the highly successful Methods in Molecular Biology series format, featuring brief introductions to the subject, lists of the necessary materials and reagents, step-by-step laboratory protocols and tips on troubleshooting and avoiding known pitfalls. Comprehensive and cutting-edge, Transgenic Wheat, Barley and Oats: Production and Characterization Protocols is an ideal guide for scientists pursuing this powerful, forward-thinking genetic strategy. 
546 |a English. 
650 0 |a Transgenic plants.  |0 http://id.loc.gov/authorities/subjects/sh90003137 
650 0 |a Wheat  |x Genetics.  |0 http://id.loc.gov/authorities/subjects/sh85146370 
650 0 |a Barley  |x Genetics.  |0 http://id.loc.gov/authorities/subjects/sh85011878 
650 0 |a Oats  |x Genetics.  |0 http://id.loc.gov/authorities/subjects/sh85093609 
650 2 |a Plants, Genetically Modified. 
650 2 |a Genetic Engineering  |x methods. 
650 2 |a Triticum  |x genetics. 
650 2 |a Hordeum  |x genetics. 
650 2 |a Avena  |x genetics. 
650 7 |a Barley  |x Genetics.  |2 fast  |0 (OCoLC)fst00827619 
650 7 |a Oats  |x Genetics.  |2 fast  |0 (OCoLC)fst01042674 
650 7 |a Transgenic plants.  |2 fast  |0 (OCoLC)fst01154677 
650 7 |a Wheat  |x Genetics.  |2 fast  |0 (OCoLC)fst01174357 
655 2 |a Laboratory Manual. 
655 4 |a Electronic books. 
700 1 |a Jones, Huw D.  |0 http://id.loc.gov/authorities/names/nb2009001030 
700 1 |a Shewry, P. R.  |q (Peter R.)  |0 http://id.loc.gov/authorities/names/n92119531 
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