Macrophages and dendritic cells : methods and protocols /

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Bibliographic Details
Imprint:	New York : Humana, ©2009.
Description:	1 online resource (xii, 368 pages) : illustrations (some color)
Language:	English
Series:	Methods in molecular biology, 1064-3745 ; v. 531 Springer protocols Methods in molecular biology (Clifton, N.J.) ; v. 531. Springer protocols (Series)
Subject:	Macrophages -- Research -- Methodology. Dendritic cells -- Research -- Methodology. Dendritic Cells. Macrophages. Laboratory Manual.
Format:	E-Resource Book
URL for this record:	http://pi.lib.uchicago.edu/1001/cat/bib/11191400

Hidden Bibliographic Details
Other authors / contributors:	Reiner, Neil E.
ISBN:	9781588299727 1588299724 9781597453967 159745396X
Digital file characteristics:	text file PDF
Notes:	Includes bibliographical references and index. English.
Summary:	In light of the critical contributions of macrophages and dendritic cells to diverse inflammatory diseases and to immunity and host defense, state-of-the-art approaches to the investigation of their behavior are essential. In Macrophages and Dendritic Cells: Methods and Protocols, expert researchers contribute laboratory protocols involving these two vital cell types functioning at the junction of the innate and acquired immune systems. The volume delves first into isolation and cell culturing then continues with topics such as phagocytosis, genetic manipulation, macrophage activation, and lipid signaling. Written in the highly successful Methods in Molecular Biology series format, chapters include brief introductions to their respective subjects, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Macrophages and Dendritic Cells: Methods and Protocols provides a timely and useful guide for both seasoned investigators and neophytes pursuing this imperative field of study.
Other form:	Print version: Macrophages and dendritic cells. New York : Humana, ©2009 9781588299727
Standard no.:	10.1007/978-1-59745-396-7

Table of Contents:

Generation of retroviral macrophage cDNA expression libraries and functional screening for surface receptors / Georgia Schäfer and Gordon D. Brown
Generation of murine growth factor-dependent long-term dendritic cell lines to investigate host-parasite interactions / Alessandra Mortellaro [and others]
Macrophage activation : classical vs. alternative / Andrea Classen, Jorge Lloberas, and Antonio Celada
Analysis of macrophage phagocytosis : quantitative assays of phagosome formation and maturation using high-throughput florescence microscopy / Benjamin E. Steinberg and Sergio Grinstein
Detection of activated Rab7 GTPase with an immobilized RILP probe / Jim Sun [and others]
Probing in vivo origins of mononuclear phagocytes by conditional ablation and reconstitution / Chen Varol, Limor Landsman, and Steffen Jung
Multivalent protein probes for the identification and characterization of cognate cellular ligands for myeloid cell surface receptors / Hsi-Hsien Lin [and others]
Exploiting Fc chimaeric proteins for the identification of ligands specific for the mannose receptor / Luisa Martinez-Pomares
The expression of exogenous genes in macrophages : obstacles and opportunities / Xia Zhang, Justin P. Edwards, and David M. Mosser
Genetic modification of murine dendritic cells by RNA transfection / Maurizio Ceppi, Enrico Schmidt, and Philippe Pierre
Recording phagosome maturation through the real-time, spectrofluorometric measurement of hydrolytic activities / Robin M. Yates, Albin Hermetter, and David G. Russell
Derivation and characterization of murine alternatively activated (M2) macrophages / Victor W.H. Ho and Laura M. Sly
Isolation and culture of human hematopoietic progenitors for studies of dendritic cell biology / Mattias Svensson
Generation, culture, and flow-cytometric characterization of primary mouse macrophages / Ulrike Schleicher and Christian Bogdan
Analyzing classical and alternative macrophage activation in macrophage/neutrophil-specific IL-4 receptor-alpha-deficient mice / Frank Brombacher [and others]
Analysis of matrix metalloproteinase secretion by macrophages / Paul T. Elkington, Justin A. Green, and Jon S. Friedland
Identification of pathogen receptors on dendritic cells to understand their function and to identify new drug targets / Lot de Witte [and others]
Stable lentiviral vector-mediated gene silencing in human monocytic cell lines / Jimmy S. Lee and Neil E. Reiner
Use of fluorescent probes to detect lipid signaling intermediates in macrophages / Vivan Kwok, Eric Vachon, and Gregory P. Downey
Large-scale phagosome preparation / Adrien F. Vinet and Albert Descoteaux
Coordinate regulation of sphingosine kinase and actin dynamics / Shankar S. Iyer and David J. Kusner.

Macrophages and dendritic cells : methods and protocols /

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