Epitope mapping protocols.

Epitope mapping protocols.

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Bibliographic Details
Edition:2nd ed. / edited by Ulrich Reineke and Mike Schutkowski.
Imprint:New York : Humana Press, ©2009.
Description:1 online resource (xiii, 456 pages, 16 unnumbered pages of plates) : illustrations (some color)
Language:English
Series:Methods in molecular biology, 1064-3745 ; 524
Springer protocols
Methods in molecular biology (Clifton, N.J.) ; v. 524.
Springer protocols (Series)
Subject:
Antigenic determinants -- Research -- Methodology.
Antigen-antibody reactions -- Research -- Methodology.
Epitopes.
Antigen-Antibody Reactions.
Epitope Mapping -- methods.
Antigenic determinants.
Format: E-Resource Book
URL for this record:http://pi.lib.uchicago.edu/1001/cat/bib/11193320
Hidden Bibliographic Details
Other uniform titles:Epitope mapping protocols.
Other authors / contributors:Reineke, Ulrich.
Schutkowski, Mike.
ISBN:9781934115176
1934115177
9781597454506
1597454508
9781607613534
1607613530
9781617379246
1617379247
Notes:Revised edition of: Epitope mapping protocols / edited by Glenn E. Morris. ©1996.
Includes bibliographical references and index.
English.
Print version record.
Summary:"Given the versatile utility of the determinination of epitopes, beneficial to a wide variety of scientists from immunologists to structural biologists to biotechnologists, the need for a thorough, state-of-the-art collection of experimental protocols is clear. In Epitope Mapping Protocols, Second Edition, expert contributors from a broad spectrum of scientific backgrounds update and expand the successful first edition with cutting-edge techniques and applications, including approaches to both antibody or B-cell epitope mapping and T-cell epitope mapping as well as a new section on the profiling of antibody signatures in biological fluids. Written in the popular Methods in Molecular Biology series format, chapters include brief introductions to the topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and Notes sections, which highlight tips on troubleshooting and avoiding known pitfalls." "Comprehensive and up-to-date, Epitope Mapping Protocols, Second Edition is a reliable and valuable reference for all those who wish to understand and further investigate the diversifying field of epitope mapping."--Jacket
Other form:Print version: Epitope mapping protocols. 2nd ed. New York : Humana Press, ©2009 9781934115176
Standard no.:10.1007/978-1-59745-450-6.
Table of Contents:
  • Preface
  • Contributors
  • Section I. B-cell Epitope Mapping
  • 1. What Is a B-Cell Epitope?
  • A. Whole Antigen Methods
  • 2. Structural Basis of Antibody-Antigen Interactions
  • 3. Epitope Mapping of Antibody-Antigen Complexes by Nuclear Magnetic Resonance Spectroscopy
  • 4. A Solid-Phase Mutual Inhibition Assay with Labeled Antigen
  • 5. Epitope Mapping by Surface Plasmon Resonance
  • 6. Proteolytic Fragmentation for Epitope Mapping
  • 7. Epitope Mapping by Proteolysis of Antigen-Antibody Complexes
  • 8. Identifying Residues in Antigenic Determinants by Chemical Modification
  • 9. Epitope Mapping by Differential Chemical Modification of Antigens
  • B. Peptide Library Approaches
  • 10. Linear B-Cell Epitope Mapping Using Enzyme-Linked Immunosorbent Assay for Libraries of Overlapping Synthetic Peptides
  • 11. Antibody Epitope Mapping Using SPOT TM Peptide Arrays
  • 12. Peptide Microarrays for Profiling of Modification State-Specific Antibodies
  • 13. Epitope Mapping Using Phage Display Peptide Libraries
  • 14. Antibody Epitope Mapping Using De Novo Generated Synthetic Peptide Libraries
  • 15. Antibody Specificity Profiling on Functional Protein Microarrays
  • 16. Peptide Microarrays for Determination of Cross-Reactivity
  • C. Profiling of Antibody Signatures in Biological Fluids
  • 17. Epitope Mapping Using Randomly Generated Peptide Libraries
  • 18. Probing the Epitope Signatures of IgG Antibodies in Human Serum from Patients with Autoimmune Disease
  • 19. Microarrayed Allergen Molecules for Diagnostics of Allergy
  • 20. Monitoring B Cell Response to Immunoselected Phage-Displayed Peptides by Microarrays
  • D. Antigen Expressed from Recombinant DNA
  • 21. Epitope Mapping Using Homolog-Scanning Mutagenesis
  • 22. Epitope Mapping by Region-Specified PCR-Mutagenesis
  • 23. Epitope Mapping Using Phage-Display Random Fragment Libraries
  • E. B-Cell Epitope Prediction
  • 24. Prediction of Linear B-cell Epitopes
  • Section II. T-cell Epitope Mapping
  • 25. Molecular Recognition of Diverse Ligands by T-Cell Receptors
  • 26. Identification of Human MHC Class I Binding Peptides using the iTOPIA TM Epitope Discovery System
  • 27. T-Cell Epitope Mapping in Mycobacterium tuberculosis Using PepMixes Created by Micro-Scale SPOT TM Synthesis
  • 28. High-Throughput T-Cell Epitope Discovery Through MHC Peptide Exchange
  • 29. T-Cell Epitope Processing (The Epitope Flanking Regions Matter)
  • 30. Identification of MHC Class II Binding Peptides: Microarray and Soluble MHC Class II Molecules
  • 31. T-Cell Epitope Mapping
  • 32. Identification and Validation of T-Cell Epitopes Using the IFN-¿ EliSpot Assay
  • Index

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