Heterotrimeric G-protein effectors /

Heterotrimeric G-protein effectors /

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Bibliographic Details
Imprint:San Diego : Academic Press, ©1994.
Description:1 online resource (xxix, 456 pages) : illustrations.
Language:English
Series:Methods in enzymology, 0076-6879 ; v. 238
Methods in enzymology ; v. 238.
Subject:
G proteins.
Clinical enzymology.
Clinical enzymology.
G proteins.
GTP-Binding Proteins [MESH]
Signal Transduction [MESH]
Electronic books.
Format: E-Resource Book
URL for this record:http://pi.lib.uchicago.edu/1001/cat/bib/11193970
Hidden Bibliographic Details
Other authors / contributors:Iyengar, Ravi.
ISBN:0121821390
9780121821395
Notes:Includes bibliographical references and indexes.
Print version record.
Summary:This volume of Methods in Enzymology and its companion Volume 237 include molecular, biological, and biochemical methods for the study of cell surface heterotrimeric G proteins and their effectors. Methods unique to signal transducing G proteins and general techniques that have been applied to the study of G protein systems are covered.
Other form:Print version: Heterotrimeric G-protein effectors. San Diego : Academic Press, ©1994 0121821390
Table of Contents:
  • cGMP Phosphodiesterase
  • Purification of Bovine Retinal cGMP Phosphodiesterase
  • Specific Peptide Probes for G-Protein Interaction with Effectors Adenylyl Cyclases
  • Determination of Adenylyl Cyclase Catalytic Activity Using Single and Double Column Procedures
  • Preparation and Use of P-Site-Targeted Affinity Ligands for Adenylyl Cyclases
  • Regulation of Ca2f-Sensitive Adenylyl Cyclases by Calcium in Vitro and in Vivo
  • Gi Assays in Transfected Cells
  • Expression and Purification of Recombinant Adenylyl Cyclases in Sf9 Cells
  • Transient Expression Assays for Mammalian Adenylyl Cyclases
  • Identification of Adenylyl Cyclases by Amplification Using Degenerate Primers Phospholipases C
  • Assays for G-Protein Regulation of Phospholipase C Activity
  • Chloride Current Assay for Phospholipase C in Xenopus Oocytes
  • Use of Cytosol-Depleted HL60 Cells for Reconstitution Studies of G-Protein-Regulated Phosphoinositide-Specific Phospholipase C-( Isozymes
  • Purification of Phosphatidylinositol Transfer Protein from Brain Cytosol for Reconstituting G-Protein-Regulated Phosphoinositide-Specific Phospholipase C-( Isozymes
  • Stimulation of Phospholipase C by G-Protein (( Subunits
  • Purification of G-Protein-Regulated Phospholipase C from Turkey Erythrocytes
  • Phospholipase C Activity in Dictyostelium discoideum Using Endogenous Nonradioactive Phosphatidylinositol 4,5-Bisphosphate as Substrate
  • Purification of Phospholipase C-(3 from Rat Brain
  • Characterization of Phospholipase C Isozymes in Bovine Retina: Purification of Phospholipase C-
  • Purification of 110 kDa Phosphoinositide Phospholipase C Activated by G-Protein (( Subunits
  • Amplification of Phosphatidylinositol-Specific Phospholipase C-( Isoforms Using Degenerate Primers Growth and Transformation
  • Analysis of Guanine Nucleotides Associated with Protooncogene Ras
  • Measuring Activation of Kinases in Mitogen-Activated Protein Kinase Regulatory Network
  • Transcriptional Activation Analysis of Oncogene Function
  • Biological Assays for Cellular Transformation. Ions and Channels
  • Monitoring of Receptor-Mediated Changes in Intracellular Calcium at the Cellular and Subcellular Levels by Microfluorimetryand Imaging
  • Combination of Microfluorimetric Monito

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