DNA microarrays for biomedical research : methods and protocols /

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Bibliographic Details
Imprint:	New York : Humana, ©2009.
Description:	1 online resource (ix, 304 pages) : illustrations
Language:	English
Series:	Methods in molecular biology, 1064-3745 ; 529 Springer protocols Methods in molecular biology (Clifton, N.J.) ; v. 529. Springer protocols (Series)
Subject:	DNA microarrays -- Research -- Methodology. Oligonucleotide Array Sequence Analysis -- methods Laboratory Manual Laboratory manuals Laboratory manuals.
Format:	E-Resource Book
URL for this record:	http://pi.lib.uchicago.edu/1001/cat/bib/11195969

Hidden Bibliographic Details
Other authors / contributors:	Dufva, Martin.
ISBN:	9781597455381 1597455385 9781934115695 193411569X 1617379417 9781617379413
Notes:	Includes bibliographical references and index. Print version record.
Summary:	DNA microarray technology has revolutionized research in the past decade. Initially an application for mRNA expression studies, the technology now has spread to other applications such as comparative genomic hybridization, SNP and mutation analysis. In DNA Microarrays for Biomedical Research: Methods and Protocols, experts explore these now commonly used applications, addressing probe design strategies, fabrication issues and providing practical examples of detailed methods for generation of high quality DNA microarray data. Chapters incorporate information on some of the largest providers of microarray, including Affymetrix, Ilumina and Agilent, and their use on a variety of applications. Composed in the highly successful Methods in Molecular Biology series format, each chapter contains a brief introduction, step-by-step methods, a list of necessary materials, and a Notes section which shares tips on troubleshooting and avoiding known pitfalls. Authoritative and highly practical, DNA Microarrays for Biomedical Research: Methods and Protocols presents a variety of protocols which can be easily reproduced, allowing researchers to gain surprising insight into the complex world of DNA microarray technology.
Other form:	Print version: DNA microarrays for biomedical research. New York : Humana, ©2009 9781934115695

Table of Contents:

Introduction to microarray technology / Martin Dufva
Probe design for expression arrays using OligoWiz / Rasmus Wernersson
Comparative genomic hybridization : microarray design and data interpretation / Richard Redon and Nigel P. Carter
Design of tag SNP whole genome genotyping arrays / Daniel A. Peiffer and Kevin L. Gunderson
Fabrication of DNA microarray / Martin Dufva
Immobilization chemistries / Sascha Todt and Dietmar H. Blohm
Fabrication using contact spotter / Annelie Waldén and Peter Nilsson
RNA preparation and characterization for gene expression studies / Michael Stangegaard
Gene expression analysis using Agilent DNA microarrays / Michael Stangegaard
Target preparation for genotyping specific genes or gene segments / Jesper Petersen, Lena Poulsen, and Martin Dufva
Genotyping of mutations in the beta-globin gene using allele specific hybridization / Lena Poulsen, Jesper Petersen, and Martin Dufva
Microarray temperature optimization using hybridization kinetics / Steve Blair [and others]
Whole-genome genotyping on bead arrays / Kevin L. Gunderson
Genotyping single nucleotide polymorphisms by multiplex minisequencing using tag-arrays
Lili Milani and Ann-Christine Syvänen
Resequencing arrays for diagnostics of respiratory pathogens / Baochuan Lin and Anthony P. Malanoski
Comparative genomic hybridization : DNA preparation for microarray fabrication / Richard Redon, Diane Rigler, and Nigel P. Carter
Comparative genomic hybridization : DNA labeling, hybridization, and detection / Richard Redon, Tomas Fitzgerald, and Nigel P. Carter
Chromatin immunoprecipitation using microarrays / Mickaël Durand-Dubief and Karl Ekwall.

DNA microarrays for biomedical research : methods and protocols /

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