Antibody phage display : methods and protocols.

Antibody phage display : methods and protocols.

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Bibliographic Details
Edition:2nd ed. / edited by Robert Aitken.
Imprint:Dordrecht ; New York : Humana Press, ©2009.
Description:1 online resource (x, 240 pages) : illustrations (some color)
Language:English
Series:Springer Protocols
Methods in molecular biology, 1064-3745 ; 562
Springer protocols (Series)
Methods in molecular biology (Clifton, N.J.) ; v. 562.
Subject:
Monoclonal antibodies.
Bacteriophages.
Antibodies, Monoclonal.
Bacteriophages.
Bacteriophages.
Monoclonal antibodies.
Laboratory Manual.
Format: E-Resource Book
URL for this record:http://pi.lib.uchicago.edu/1001/cat/bib/11197384
Hidden Bibliographic Details
Other authors / contributors:Aitken, Robert, 1960-
ISBN:9781603273022
1603273026
9781603273015
1603273018
Notes:Previous edition: 2002.
Includes bibliographical references and index.
Restrictions unspecified
Electronic reproduction. [Place of publication not identified] : HathiTrust Digital Library, 2010.
Master and use copy. Digital master created according to Benchmark for Faithful Digital Reproductions of Monographs and Serials, Version 1. Digital Library Federation, December 2002. http://purl.oclc.org/DLF/benchrepro0212
digitized 2010 HathiTrust Digital Library committed to preserve
Print version record.
Summary:The aim of this handbook is to provide a resource that will guide readers in the design and execution of experiments based around antibody phage display
Other form:Print version: Antibody phage display. 2nd ed. Dordrecht ; New York : Humana Press, ©2009 9781603273015
Table of Contents:
  • Antibody phage display: overview of a powerful technology that has quickly translated to the clinic / B. Kotlan and M.C. Glassy
  • Design and construction of synthetic phage-displayed Fab libraries / J. Bostrom and G. Fuh
  • Affinity isolation of antigen-specific circulating B cells for generation of phage display-derived human monoclonal antibodies / H.J. Ditzel
  • Construction of phage antibody repertoires from the blood of West Nile virus-infected donors / M. Throsby and J. de Kruif
  • Anti-beta(2)GP-I and anti-prothrombin antibodies generated by phage display / J. Cabiedes and A.R. Cabral
  • The generation and selection of single-domain, v region libraries from nurse sharks / M.F. Flajnik and H. Dooley
  • The isolation of scFvs against small target molecules / I. Shaw and M. Kane
  • Display and selection of scFv antibodies on HEK-293T cells / M. Ho and I. Pastan
  • Isolation of scFvs that inhibit the NS3 protease of hepatitis C virus by a combination of phage display and a bacterial genetic screen / L. Nahary, A. Trahtenherts and I. Benhar
  • Guided selection methods through chain shuffling / B. Guo-Qiang and H. Xian-Li
  • Sequential antigen panning for selection of broadly cross-reactive HIV-1-neutralizing human monoclonal antibodies / M.Y. Zhang and D.S. Dimitrov
  • Selection of antibodies able to rapidly enter mammalian cells / M.A. Poul
  • Identification of the specificity of isolated phage display single-chain antibodies using yeast two-hybrid screens / N. Rasmussen and H.J. Ditzel
  • Generation of bispecific and tandem diabodies / S.M. Kipriyanov
  • Intrabody expression in eukaryotic cells / L. Guglielmi and P. Martineau
  • High-level periplasmic expression and purification of scFvs / S.M. Kipriyanov
  • Expression of single-chain Fv fragments in E. coli cytoplasm / L. Guglielmi and P. Martineau
  • High-level expression of a phage display-derived scFv in Pichia pastoris / L.M. Damasceno [and others].

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