Microarray analysis of the physical genome : methods and protocols /

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Bibliographic Details
Imprint:	New York : Humana, ©2009.
Description:	1 online resource (x, 223 pages) : illustrations
Language:	English
Series:	Methods in molecular biology, 1064-3745 ; v. 556 Springer protocols Methods in molecular biology (Clifton, N.J.) ; v. 556. Springer protocols (Series)
Subject:	DNA microarrays -- Laboratory manuals. Genomics -- Laboratory manuals. Genome. Microarray Analysis -- methods. DNA -- analysis. Genetic Phenomena. DNA microarrays. Genomics. Laboratory Manual. Laboratory manuals.
Format:	E-Resource Book
URL for this record:	http://pi.lib.uchicago.edu/1001/cat/bib/11197910

Hidden Bibliographic Details
Other authors / contributors:	Pollack, Jonathan R.
ISBN:	9781603271912 1603271910 9781603271929 1603271929
Digital file characteristics:	text file PDF
Notes:	Includes bibliographical references and index. English. Print version record.
Summary:	In the early years of microarray technology, efforts were directed mainly at profiling expressed genes, while recently the microarray platform has been adapted into diverse applications directed toward the investigation of the physical genome. In Microarray Analysis of the Physical Genome: Methods and Protocols, experts in the field cover DNA microarray applications for the detection and characterization of genomic DNA-associated copy number alteration, loss of heterozygosity (LOH), cytosine methylation, protein binding sites, regulatory elements, and replication timing, with an emphasis on higher eukaryote (animal) and cancer genomes. Written in the highly successful Methods in Molecular Biology series format, chapters include a brief introduction to their respective subjects, lists of the necessary materials and reagents, step-by-step protocols, and time-saving notes on troubleshooting and avoiding known pitfalls. Authoritative and easy-to-use, Microarray Analysis of the Physical Genome: Methods and Protocols is of great value to the molecular biologist or computational biologist interested in understanding the principles of these analyses or in planning future experiments using microarrays to characterize the physical genome.
Other form:	Print version: Microarray analysis of the physical genome. New York : Humana, ©2009 9781603271912
Standard no.:	10.1007/978-1-60327-192-9

Table of Contents:

Preface
Contributors
1. Introduction
2. Comparative Genomic Hybridization on BAC Arrays
3. Comparative Genomic Hybridization on Spotted Oligonucleotide Microarrarys
4. Comparative Genomic Hybridization by Representational Oligonucleotide Microarray Analysis
5. Application of Obligonucleotides Arrays for Coincident Comparative Genomic Hybridization, Polidy Status and Loss of Heterozygosity Studies in Human Cancers
6. Molecular Inversion Probe Assay for Allelic Quantitation
7. A Whole-Genome Amplification Protocol for a Wide Variety of DNAs, Including Those from Formalin-Fixed and Paraffin-Embedded Tissue
8. Algorithms for Calling Gains and Losses in Array CGH Data
9. Methylation Analysis by Microarray
10. Methylation Analysis by DNA Immunoprecipation (MeDIP)
11. Combining Chromatin Immunoprecipitation and Oligonucleotide Tiling Arrays (ChIP-Chip) for Functional Genomic Studies
12. ChIP-Chip: Algorithms for Calling Binding Sites
13. Mapping Regulatory Elements by DNasel Hypersensitivity Chip (DNase-Chip)
14. Microarray Analysis of DNA Replication Timing
15. Integration of Diverse Microarray Data Types
Index

Microarray analysis of the physical genome : methods and protocols /

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