Mitochondrial biogenesis and genetics. Part B /

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Bibliographic Details
Imprint:San Diego : Academic Press, ©1996.
Description:1 online resource (xxx, 621 pages) : illustrations.
Language:English
Series:Methods in enzymology, 0076-6879 ; v. 264
Methods in enzymology ; v. 264.
Subject:
Format: E-Resource Book
URL for this record:http://pi.lib.uchicago.edu/1001/cat/bib/11198428
Hidden Bibliographic Details
Other authors / contributors:Attardi, Giuseppe.
Chomyn, Anne.
ISBN:012182165X
9780121821654
Notes:Includes bibliographical references and indexes.
Summary:The critically acclaimed laboratory standard for forty years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerlyawaited, frequently consulted, and praised by researchers and reviewers alike. More than 260 volumes have been published (all of them still in print) and much of the material is relevant even today--truly an essential publication for researchers in all fields of life sciences. Key Features * This volume covers methodology used in * Mitochondrial gene expression * Mitochondrial genetics and gene manipulation * Mitochondrial diseases and aging.
Table of Contents:
  • In Vivo and in Vitro Approaches to Study of Mitochondrial Gene Expression
  • In Vivo Footprinting of Human Mitochondrial DNA in Cultured Cell Systems
  • Genomic Footprinting of Mitochondrial DNA
  • I. In Organello Analysis of ProteinMitochondrial DNA Interactions in Bovine Mitochondria
  • Genomic Footprinting of Mitochondrial DNA
  • II. In Vivo Analysis of ProteinMitochondrial DNA Interactions in Xenopus laevis Eggs and Embryos
  • In Organello Mitochondrial Protein and RNA Synthesis Systems from Saccharomyces cerevisiae
  • III. In Organello RNA Synthesis System from HeLa Cells
  • In Organello RNA Synthesis System from Mammalian Liver and Brain
  • Transcription in Vitro with Saccharomyces cerevisiae Mitochondrial RNA Polymerase
  • Reactions Catalyzed byGroup II Introns in Vitro
  • Genetic and Biochemical Approaches for Analysis of Mitochondrial RNase P from Saccharomyces cerevisiae
  • RNA Editing in Trypanosomatid Mitochondria
  • Purification of Mitochondrial DNA from Human Cell Cultures and Placenta
  • Mitochondrial DNA Transcription Initiation and Termination Using Mitochondrial Lysates from Cultured Human Cells
  • Mapping Promoters in Displacement-Loop Region of Vertebrate Mitochondrial DNA
  • Isolation and Characterization of Vertebrate Mitochondrial Transcription Factor A Homologs
  • Isolation and Assay of Mitochondrial Transcription Termination Factor from Human Cells
  • Isolation of Mitochondrial tRNAs from Human Cells
  • Analysis of Aminoacylation of Human Mitochondrial tRNAs
  • In Vivo Labeling and Analysis of Human Mitochondrial Translation Products
  • Mitochondrial Protein Synthesis in Rat Brain Synaptosomes
  • Immunoprecipitation of Human Mitochondrial Translation Products with Peptide-Specific Antibodies
  • Genetic Strategies for Identification of Mitochondrial Translation Factors in Saccharomyces cerevisiae
  • Bovine Mitochondrial Ribosomes
  • Bovine Mitochondrial Initiation and Elongation Factors
  • Mitochondrial Genetics and Gene Manipulation
  • Transformation of Saccharomyces cerevisiae Mitochondria Using the Biolistic Gun
  • Genetics and Transformation of Mitochondria in the Green Alga Chlamydomonas
  • Isolation of Avian Mitochondrial DNA-Less Cells
  • Isolation of Human Cell Lines Lacking Mitochondrial DNA
  • Mitochondria-Mediated Transformation of Human rho Cells
  • Platelet-Mediated Transformation of Human Mitochon drial DNA-Less Cells
  • Use of Ethidium Bromide to Manipulate Ratio of Mutated and Wild-Type Mitochondrial DNA in Cultured Cells
  • Construction of Heteroplasmic Mice Containing Two Mitochondrial DNA Genotypes by Micromanipulation of Single-Cell Embryos
  • Mitochondr