Oxygen radicals in biological systems. Part D /

Oxygen radicals in biological systems. Part D /

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Bibliographic Details
Imprint:San Diego : Academic Press, ©1994.
Description:1 online resource (xxxiii, 704 pages) : illustrations.
Language:English
Series:Methods in enzymology, 0076-6879 ; v. 234
Methods in enzymology ; v. 234.
Subject:
Active oxygen.
Radicals (Chemistry)
Active oxygen.
Radicals (Chemistry)
Antioxidants [MESH]
Free Radicals [MESH]
Electronic books.
Format: E-Resource Book
URL for this record:http://pi.lib.uchicago.edu/1001/cat/bib/11198459
Hidden Bibliographic Details
Other authors / contributors:Packer, Lester.
ISBN:0121821358
9780121821357
Notes:Includes bibliographical references and indexes.
Summary:Since biological tissues are unstable in an oxygen atmosphere, a great deal of effort is expended by organisms to metabolically limit or repair oxidative tissue damage. This volume of Methods in Enzymology and its companion Volume 233 present methods developed to investigate the roles of oxygen radicals and antioxidants in disease. It includes techniques and assays of the activity of biothiols in soluble systems, their interaction with lipids and proteins, and the various new thiol enzymes, such as thioltransferases, being elucidated. Antioxidant reactions and gene regulation are also included.
Table of Contents:
  • Generation, Detection, and Characterization of Oxygen Radicals in Chemistry, Biochemistry, and Intermediate States of Reduction
  • Pulse Radiolysis
  • Pulse Radiolysis for Investigation of Nitric Oxide-Related Reactions
  • Sulfhydryl Free Radical Formation by Sonolysis, by Radiolysis, and Thermally: Vitamin A, Curcumin, Muconic Acid, and Related Conjugated Olefins and References
  • Suppression of Hydroxyl Radical Reactions in Biological Systems: Considerations Based on Competition Kinetics
  • Deoxyribose Assay for Detecting Hydroxyl Radicals
  • Detection of Hydroxyl Radicals by Aromatic Hydroxylation
  • Measurement of Iron and Copper in Biological Systems: Blemycin and Copper-Phenanthroline Assays
  • Oxygen Radicals Generation and DNA Scission by Anticancer and Synthetic Quinones
  • Spin Trapping of Hydroxyl Radicals in Biological Systems
  • In Vivo Detection of Radical Adducts by Electron Spin Resonance
  • Spin Trapping of Alcohol-Derived Radicals in Microsomes and Reconstituted Systemsby Electron Spin Resonance
  • In Vivo Visualization of Oxygen Radical-Dependent Photoemission
  • In Vivo Electron Paramagnetic Resonance Imaging of Skin
  • Electron Spin Resonance Assay of Ascorbate-Free Radicals in Vivo
  • Measurement of Superoxide Reaction by Chemiluminescence
  • Isolation, Characterization, and Assay of Enzymes or Substances Involved in Formation or Removal of Oxygen Radicals
  • Mass Spectrometry of Prostanoids: F2-Isoprostances Produced by NonCyclooxygenase Free Radical-Catalyzed Mechanism
  • Quantifying Aldehydes and Distinguishing Aldehydic Product Profiles from Autooxidation and Ozonation of Unsaturated Fatty Acids
  • Ferrous Ion Oxidation in Presence of Ferric Ion Indicator Xylenol Orange for Measurement of Hydroperoxides
  • Ferrylmyoglobin Formation: Chemical Reactivity Toward Electron-Donating Compounds
  • Enzymatic and Immunological Measurements of Soluable and Membrane-Bound Phospholipid-Hydroperoxide Glutathione Peroxidase
  • Targeting Superoxide Dismutase by Gene and Protein Engineering
  • Assays of Plasma and Membrane NADPH Oxidase
  • Oxidative Chemistry of Peroxynitrite
  • Nitric Oxide Assay Using Hemoglobin Method
  • Nitric-Oxide Synthase Assays
  • Isoforms of Nitric-Oxide Synthase: Purification and Regulation
  • Purification, Cloning, and Expression of Nitric-Oxide Synthase
  • Methods for Assessing Molecular, C

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