DNA electrophoresis : methods and protocols /

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Bibliographic Details
Imprint:	New York : Humana Press, [2013] ©2013
Description:	1 online resource (x, 299 pages) : illustrations (some color)
Language:	English
Series:	Methods in Molecular Biology, 1940-6029 ; 1054 Methods in molecular biology (Clifton, N.J.) ; v. 1054.
Subject:	Electrophoresis -- Laboratory manuals. DNA -- Laboratory manuals. Nucleic Acids. Electrochemical Techniques. Publication Formats Natural Science Disciplines. Investigative Techniques Chemistry Techniques, Analytical. Analytical, Diagnostic and Therapeutic Techniques and Equipment Disciplines and Occupations Nucleic Acids, Nucleotides, and Nucleosides Publication Characteristics Chemicals and Drugs Chemistry. Methods. Electrophoresis. Laboratory Manuals DNA. DNA. Electrophoresis. Laboratory manuals.
Format:	E-Resource Book
URL for this record:	http://pi.lib.uchicago.edu/1001/cat/bib/11202807

Hidden Bibliographic Details
Other authors / contributors:	Makovets, Svetlana, editor.
ISBN:	9781627035651 1627035656 1627035648 9781627035644 9781627035644
Digital file characteristics:	text file PDF
Notes:	Includes bibliographical references and index. English. Print version record.
Summary:	In DNA Electrophoresis: Methods and Protocols, expert researchers in the field detail many of the methods which are now commonly used to study DNA using electrophoresis as the major approach. A powerful tool that allows separating DNA molecules according to their size and shape, this volume includes methods and techniques such as 2-dimentional gel electrophoresis as the major approach. These include methods and techniques such as 2-dimentional gel electrophoresis, DNA electrophoresis under conditions in which DNA molecules are completely or partially denatured during the runs, Pulse Field Gel Electrophoresis, electrophoresis coupled to fluorescence in situ hybridization, as well as protein-DNA interactions studied using electrophoreses. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, DNA Electrophoresis: Methods and Protocols aids scientists in continuing to study DNA dynamics both in live cells and in test tubes.
Other form:	Print version: DNA electrophoresis 1627035648
Standard no.:	10.1007/978-1-62703-565-1

Table of Contents:

DNA electrophoresis : historical and theoretical perspectives / Gareth A. Roberts and David T.F. Dryden
Basic DNA electrophoresis in molecular cloning : a comprehensive guide for beginners / Svetlana Makovets
Analysis of branched DNA replication and recombination intermediates from prokaryotic cells by two-dimensional (2D) native-native agarose gel electrophoresis / Nicholas P. Robinson
Analysis of DNA structures from eukaryotic cells by two-dimensional native-native DNA agarose gel electrophoresis / Andreas S. Ivessa
Directionality of replication fork movement determined by two-dimensional native-native DNA agarose gel electrophoresis / Andreas S. Ivessa
Native/denaturing two-dimensional DNA electrophoresis and its application to the analysis of recombination intermediates / Jessica P. Lao, Shangming Tang, and Neil Hunter
Plasmid DNA topology assayed by two-dimensional agarose gel electrophoresis / Jorge B. Schvartzman [and others]
Neutral glyoxal gel electrophoresis method for the detection and semi-quantitation of DNA single-strand breaks / Brian Pachkowski and Jun Nakamura
Denaturing gradient gel electrophoresis (DGGE) / Fiona Strathdee and Andrew Free
Polyacrylamide temperature gradient gel electrophoresis / Viktor Viglasky
Separation of DNA oligonucleotides using denaturing urea PAGE / Fiona Flett and Heidrun Interthal
Pulsed-field gel electrophoresis of bacterial chromosomes / Julia S.P. Mawer and David R.F. Leach
Resolution of budding yeast chromosomes using pulsed-field gel electrophoresis / Aziz El Hage and Jonathan Houseley
Analysis of DNA damage via single-cell electrophoresis / Diana Anderson and Julian Laubenthal
Fluorescence in situ hybridization on electrophoresed cells to detect sequence specific DNA damage / Julian Laubenthal and Diana Anderson
Analysis of DNA-protein interactions using PAGE : band-shift assays / Lynn Powell
Assaying cooperativity of protein-DNA interactions using agarose gel electrophoresis / Tanya L. Williams and Daniel L. Levy
DNA bending by proteins : utilizing plasmid pBendAT as a tool / Fenfei Leng
Using PCR coupled to PAGE for detection and semiquantitative evaluation of telomerase activity / Laura Gardano.

DNA electrophoresis : methods and protocols /

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