Flow cytometry : principles, methodology and applications /

Flow cytometry : principles, methodology and applications /

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Bibliographic Details
Imprint:Hauppauge, New York : Nova Science Publishers, Inc., 2013.
Description:1 online resource
Language:English
Series:Cell Biology Research Progress
Cell biology research progress.
Subject:
Flow cytometry.
Flow cytometry.
Electronic books.
Format: E-Resource Book
URL for this record:http://pi.lib.uchicago.edu/1001/cat/bib/11203061
Hidden Bibliographic Details
Other authors / contributors:Papandreou, Stefanos.
ISBN:9781628087109
1628087102
9781628087093
1628087099
Notes:Includes index.
Print version record.
Summary:Flow cytometry is a method to conduct a multiparameter analysis of cells suspended in liquid and passing through a laser beam. As cells pass through the flow chamber they are hit with the laser light beam which is scattered in different directions and recorded as forward light scatter and side light scatter. In this book, the authors discuss the principles, methodology and applications of flow cytometry. Topics include flow cytometry and epifluorescence analyses of freshwater bacterio- and virioplankton communities; application of flow cytometry in phenotyping, analysis and functional characte.
Other form:Print version: Flow cytometry. Hauppauge, New York : Nova Science Publishers, Inc., 2013 9781628087093
Table of Contents:
  • ""FLOW CYTOMETRY. PRINCIPLES, METHODOLOGY AND APPLICATIONS""; ""FLOW CYTOMETRY. PRINCIPLES, METHODOLOGY AND APPLICATIONS""; ""Library of Congress Cataloging-in-Publication Data""; ""Contents""; ""Preface""; ""Chapter 1: A Few Tests Prior to Flow Cytometry and Epifluorescence Analyses of Freshwater Bacterio -And Virioplankton Communities""; ""Abstract""; ""Introduction""; ""Materials and Methods""; ""Results""; ""Discussion""; ""Conclusion""; ""Acknowledgment""; ""References""
  • ""Chapter 2: Application of Flow Cytometry in Phenotyping, Analysis and Functional Characterization of T Regulatory and Immune Suppressor Cells: A Focus on Multiple Myeloma Immune Regulatory Biology""""Abstract""; ""Introduction""; ""Ontogeny of T Regulatory Cells""; ""FoxP3 Gene and Their Role in T Regulatory Cell Differentiation""; ""Role of FoxP3 in T Regulatory Cells""; ""Suppressive Mechanisms of T Regulatory Cells""; ""Delineation of Phenotypic Features of CD4 T Regulatory Cells""; ""Subsets of CD4 T Regulatory Cells""
  • ""Assessment of Suppressive Function of T Regulatory Cells: Flow Cytometry Versus Thymidine Uptake Method""""CD8 T Regulatory Cells: A Novel Suppressor""; ""Suppressive Mechanisms of CD8 Treg Cell Subsets""; ""Myeloid-Derived Suppressor Cells""; ""Immune Regulatory Biology of Multiple Myeloma""; ""Conclusion""; ""Acknowledgment""; ""References""; ""Chapter 3: Multicolor Panel Design Strategies in Rare Event Analysis: Dendritic Cells and Regulatory T Cells""; ""Abstract""; ""1. Introduction""; ""2. How to Avoid Background (“Noiseâ€?)""; ""3. Reagent Selection""
  • ""4. Limiting Spectral Overlap""""5. Technical Limitations of Tandem Dyes""; ""6. Appropriate Controls to Validate Your Experiment""; ""7. How Many Events Must be Processed?""; ""8. Multicolor Panel Design to Detect Rare Events: Two Examples""; ""Conclusion""; ""References""; ""Chapter 4: Light-Scattering Flow Cytometry: Advanced Characterization of Individual Particle Morphology""; ""Abstract""; ""Introduction""; ""Instrument""; ""Application""; ""Conclusion""; ""Acknowledgments""; ""References""; ""Chapter 5: Applications of Flow Cytometry in Environmental Sciences: Inspiring Examples""
  • ""Abstract""""Introduction""; ""Microbial Pollution of Drinking Water""; ""Viability of Bees Spermatozoa""; ""Monitoring the Cyanobacteria""; ""Conclusion""; ""References""; ""Index""

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