Methicillin-resistant Staphylococcus aureus (MRSA) protocols /

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Bibliographic Details
Edition:2nd ed.
Imprint:New York : Humana Press : Springer, ©2014.
Description:1 online resource (x, 353 pages) : illustrations (some color)
Language:English
Series:Methods in molecular biology ; v. 1085
Methods in molecular biology (Clifton, N.J.) ; v. 1085.
Subject:
Format: E-Resource Book
URL for this record:http://pi.lib.uchicago.edu/1001/cat/bib/11207363
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Varying Form of Title:MRSA protocols
Other authors / contributors:Ji, Yinduo.
ISBN:9781627036641
1627036644
9781627036634
1627036636
Digital file characteristics:text file PDF
Notes:Includes bibliographical references and index.
English.
Summary:Presenting the most up-to-date techniques for the detection, genotyping, and investigation of methicillin-resistant S. aureus (MRSA), this second edition of Methicillin-Resistant Staphylococcus aureus (MRSA) Protocols collects chapters that utilize the power of complete genomic sequences and advanced high-throughput technologies that have pushed this field to its present state. These enable the development of specific and rapid diagnosis methods, the investigation and elucidation of mechanisms of bacterial evolution to antibiotic resistance and pathogenicity, and the identification of novel targets to develop more potent therapeutic and/or preventive agents. Written for the Methods in Molecular Biology series, numerous chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and fully updated, Methicillin-Resistant Staphylococcus aureus (MRSA) Protocols, Second Edition serves as a key reference for researchers attempting to set up a new method to study MRSA or even for technicians and scientists working on other pathogens.
Other form:Printed edition: 9781627036634
Standard no.:10.1007/978-1-62703-664-1
Table of Contents:
  • Clinical, epidemiologic, and laboratory aspects of methicillin-resistant Staphylococcus aureus infections / Elizabeth L. Palavecino
  • Community-associated methicillin-resistant Staphylococcus aureus case studies / Madeleine G. Sowash and Anne-Catrin Uhlemann
  • Rapid methods for detection of MRSA in clinical specimens / Elizabeth L. Palavecino
  • Immunofluorescence microscopy for the detection of surface antigens in methicillin-resistant Staphylococcus aureus (MRSA) / Yekaterina Timofeyeva, Ingrid L. Scully, and Annaliesa S. Anderson
  • Internal transcribed spacer (ITS)-PCR identification of MRSA / Shin-ichi Fujita
  • Pulsed-field gel electrophoresis typing of Staphylococcus aureus isolates / Yiping He, Yanping Xie, and Sue Reed
  • Multilocus sequence typing (MLST) of Staphylococcus aureus / Nicholas A. Saunders and Anne Holmes
  • Staphylococcal cassette chromosome mec (SCCmec) analysis of MRSA / Teruyo Ito [and others]
  • Genetic interruption of target genes for investigation of virulence factors / Adhar C. Manna
  • Molecular analysis of staphylococcal superantigens / Wilmara Salgado-Pabón, Laura C. Case-Cook, and Patrick M. Schlievert
  • Investigation of Staphylococcus aureus adhesion and invasion of host cells / Junshu Yang and Yinduo Ji
  • Investigation of biofilm formation in clinical Isolates of Staphylococcus aureus / James E. Cassat, Mark S. Smeltzer, and Chia Y. Lee
  • Transcriptomic analysis of Staphylococcus aureus using microarray and advanced next-generation RNA-seq technologies / Ting Lei, Aaron Becker, and Yinduo Ji
  • Proteomic approach to investigate pathogenicity and metabolism of methicillin-resistant Staphylococcus aureus / Patrice François [and others]
  • Metabolomic investigation of methicillin-resistant Staphylococcus aureus / Ting Lei [and others]
  • Treatment of infections due to resistant Staphylococcus aureus / Gregory M. Anstead, Jose Cadena, and Heta Javeri
  • Anti-infective drug development for MRSA / Anu Daniel
  • Animal models in drug development for MRSA / Andrea Marra.