Epiblast stem cells : methods and protocols /

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Bibliographic Details
Imprint:	[Totowa, New Jersey] : Humana Press, [2013] ©2013
Description:	1 online resource (xi, 216 pages) : illustrations (some color)
Language:	English
Series:	Methods in molecular biology, methods and protocols, 1064-3745 ; 1074 Methods in molecular biology (Clifton, N.J.) ; v. 1074,
Subject:	Embryonic stem cells. Embryonic stem cells -- Research. Stem Cells. Embryonic Structures. Publication Formats Anatomy. Publication Characteristics Cells. Germ Layers. Embryonic Stem Cells. Laboratory Manuals Embryonic stem cells. Embryonic stem cells -- Research.
Format:	E-Resource Book
URL for this record:	http://pi.lib.uchicago.edu/1001/cat/bib/11207493

Hidden Bibliographic Details
Other authors / contributors:	Alberio, Ramiro, editor.
ISBN:	9781627036283 1627036288 162703627X 9781627036276 9781627036276
Digital file characteristics:	text file
Notes:	Includes bibliographical references and index. English.
Summary:	In Epiblast Stem Cells: Methods and Protocols, expect researchers in the field provide a detailed collection of techniques and protocols useful to the study of the biology of the pluripotent epiblast. These include methods and techniques used to study epiblast development in different amniotes. This collection brings together contributions from the fields of embryology, stem cell biology and developmental biology together, providing a single volume with detailed procedures for the isolation and culture of epiblasts at different stages of development, and techniques for the study of differentiation into specific lineages.
Other form:	Print version: Epiblast stem cells. New York : Humana Press, ©2013 162703627X
Standard no.:	10.1007/978-1-62703-628-3

Table of Contents:

Generation and Characterization of Epiblast Stem Cells from Blastocyst-stage Mouse Embryos
Reversion of Mouse Post-Implantation Epiblast Stem Cells to a Naïve Pluripotent State by Modulation of Signalling Pathways
Converting Mouse Epiblast Stem Cells into Mouse Embryonic Stem Cells by using Small Molecules
Isolation and Culture of Rabbit Embryonic Stem Cells
Goat Embryonic Stem-like Cell Derivation and Characterization
Derivation and Culture of Canine Embryonic Stem Cells
Isolation and Culture of Porcine Embryonic Stem Cells
Isolation and Culture of Pig Epiblast Stem Cells
Isolation and Culture of Bovine Embryonic Stem Cells
Analysis of Molecular Markers for Staging Peri-gastrulating Bovine Embryos
Chicken Embryonic Stem Cells: Establishment and Characterization
Manipulating the Avian Epiblast and Epiblast-derived Stem Cells
Stepwise Differentiation from Naïve State Pluripotent Stem Cells to Functional Primordial Germ Cells through an Epiblast-like State
Isolation and Culture of Porcine Neural Progenitor Cells from Embryos and Pluripotent Stem Cells
Derivation of Neural Precursors from Bovine Pre-implantation Embryos.

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