Clinical applications of PCR /

Clinical applications of PCR /

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Bibliographic Details
Imprint:Totowa, N.J. : Humana Press, ©1998.
Description:1 online resource (x, 353 pages) : illustrations
Language:English
Series:Methods in molecular medicine ; 16
Methods in molecular medicine ; 16.
Subject:
Polymerase chain reaction -- Diagnostic use.
Polymerase Chain Reaction -- methods
Polymerase chain reaction -- Diagnostic use
Laboratory Manual
Laboratory manuals.
Format: E-Resource Book
URL for this record:http://pi.lib.uchicago.edu/1001/cat/bib/11207923
Hidden Bibliographic Details
Varying Form of Title:Clinical applications of polymerase chain reaction
Other authors / contributors:Lo, Y. M. Dennis.
ISBN:9781592596003
1592596002
9781489943514
0896034992
9780896034990
0896033597
9780896033597
Notes:Includes bibliographical references and index.
Electronic reproduction. [Place of publication not identified] : HathiTrust Digital Library, 2010.
Master and use copy. Digital master created according to Benchmark for Faithful Digital Reproductions of Monographs and Serials, Version 1. Digital Library Federation, December 2002. http://purl.oclc.org/DLF/benchrepro0212
English.
digitized 2010 HathiTrust Digital Library committed to preserve
Print version record.
Summary:Clinical Applications of PCR offers an unprecedented collection of core PCR techniques for the study and diagnosis of human diseases. Cutting-edge and essential for today's diagnostic laboratories, these techniques heavily utilize nonisotopic, solution phase, and in situ amplification methods. A significant number of chapters describe applications exploiting the exquisite sensitivity of PCR in the detection of rare or single cells, as in identifying fetal cells circulating in maternal blood, preimplantation embryo diagnosis, or detecting circulating cancer cells. The methods described in Clinical Applications of PCR will well serve diverse clinical specialties ranging from hematology/oncology, human genetics, and microbiology, to virology, pathology, and infectious diseases. The book repeatedly demonstrates the power of PCR-its high sensitivity, specificity, and ability to rapidly discriminate sequence variations.
Other form:Print version: Clinical applications of PCR. Totowa, N.J. : Humana Press, ©1998
Standard no.:10.1385/0896034992
Table of Contents:
  • Introduction to the polymerase chain reaction / Y.M. Dennis Lo
  • Setting up a PCR laboratory / Y.M. Dennis Lo
  • Amplification from archival materials / Y.M. Dennis Lo
  • Quantitative PCR / Luc Raeymaekers
  • Mutation screening using PCR-SSCP : silver staining and isotopic protocols / Philip J. Saker
  • Multiple fluorescence-based PCR-SSCP analysis with primer-, post-, and internal labeling / Hiroyuki Iwahana, Mitsuo Itakura
  • Amplification refractory mutation system / Y.M. Dennis Lo
  • Artificial restriction fragment length polymorphism (A-RFLP) analysis / Y.M. Dennis Lo, Virginia A. Horton
  • Long Range PCR / William Waggott
  • Generation of labeled probes by PCR / Y.M. Dennis Lo, Shu F. An
  • PCR for the detection of minority DNA populations / Y.M. Dennis Lo
  • In situ amplification / John O'Leary
  • Sequencing of PCR products / Paul Moss, Swee Lay Thein
  • Reverse transcriptase-polymerase chain reaction (RT-PCR) / Rajko Kusec
  • PCR amplification of minisatellite DNA for the detection of mixed chimerism after bone marrow transplantation / Etienne Roux
  • Diagnosis of mitochondrial disorders using the PCR / Karl J. Morten
  • PCR analysis of CD44 variants in tumors / John Bolodeoku
  • Detection of circulating solid tumor cells by reverse transcriptase polymerase chain reaction / Susan A. Burchill
  • Detection of circulating tumor cells using immunobead-PCR / Jennifer E. Hardingham
  • Single Cell PCR : theory, practice, and clinical applications / Ian Findlay
  • PCR-based noninvasive prenatal diagnosis using fetal cells in maternal circulation / Y.M. Dennis Lo
  • Noninvasive prenatal diagnosis using a single fetal nucleated erythrocyte isolated by micromanipulation from maternal blood / Akihiko Sekizawa, Hiroshi Saito, Takumi Yanaihara
  • Diagnosis of chromosomal aneuploidies using quantitative fluorescent PCR / Barbara Pertl, Matteo Adinolfi
  • Detection of the Hepatitis C virus by RT-PCR / Christopher John Healy, Steven Read
  • Application of PCR to the detection of M. tuberculosis in sputum samples / Majella Maher [and others]
  • Microsatellite analysis in human disease / John Koreth, Christopher J. Bakkenist, James O'D. McGee.

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