$2D PAGE. Volume 425 : sample preparation and fractionation /$

2D PAGE. sample preparation and fractionation / Volume 425 :

Saved in:

Bibliographic Details
Imprint:	Totowa, N.J. : Human Press, ©2008.
Description:	1 online resource : illustrations
Language:	English
Series:	Methods in molecular biology ; v. 425 Methods in molecular biology (Clifton, N.J.) ; volume 425.
Subject:	Proteomics. Two-dimensional electrophoresis. Sample preparation (Chemistry) Proteomics -- methods Analytic Sample Preparation Methods Chemical Fractionation -- methods Electrophoresis, Gel, Two-Dimensional -- methods Proteomics Electrophoresis, Gel, Two-Dimensional Sample preparation (Chemistry) Proteomics Two-dimensional electrophoresis Laboratory Manual Laboratory manuals Laboratory manuals.
Format:	E-Resource Book
URL for this record:	http://pi.lib.uchicago.edu/1001/cat/bib/11208705

Hidden Bibliographic Details
Varying Form of Title:	Two-dimensional PAGE sample preparation and fractionation
Other authors / contributors:	Posch, Anton.
ISBN:	9781603272100 1603272100
Notes:	Includes bibliographical references and index. Print version record.
Summary:	The complex nature of proteins often requires a multitude of sample preparation options. In 2D PAGE: Sample Preparation and Fractionation, Volumes 1 & 2, Dr. Anton Posch presents a broad coverage of the principles and recent developments of sample preparation and fractionation tools in Expression Proteomics and specifically for two-dimensional electrophoresis (2-DE). Detailed laboratory methods describe the process of sample fractionation used to reduce the complexity of the protein sample and the methods of 2-DE analysis and a broad variety of gel-free mass spectrometry-based technologies to detect previously undetectable proteins. Vital information regarding protein isolation and preparation for reproducible proteome analysis previously overlooked by scientists has been clearly laid out in this pioneering two-volume set. Volume 1 focuses on the basics of sample preparation and protein labeling techniques with an emphasis on fractionation by chemical reagents, chromatography, and electrophoresis methods. The inclusion of meticulous enrichment strategies further aids the protein researcher. Invaluable for students of Biochemistry, Biomedicine, Biology and Genomics and essential for the practicing scientists, 2D PAGE: Sample Preparation and Fractionation, Volumes 1 & 2 delve into the study of proteins with comprehensive and in depth methods and protocols to provide a greater understanding of this intricate and complicated field.
Other form:	Print version: 2D PAGE. Totowa, N.J. : Human Press, ©2008 9781603270649 1603270647

Description
Summary:	Focusing on two-dimensional electrophoresis (2-DE), this book sets forth the principles and recent developments of sample preparation and fractionation tools in expression proteomics. Step-by-step instructions alongside detailed illustrations enable you to quickly learn how to perform the methods yourself. Tricks of the trade and notes on troubleshooting and avoiding pitfalls help you ensure successful results every time. The methods covered in this book are not limited to 2-DE; they can also be applied to a broad range of protein separation techniques.
Physical Description:	1 online resource : illustrations
Bibliography:	Includes bibliographical references and index.
ISBN:	9781603272100 1603272100