RNA folding : methods and protocols /

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Bibliographic Details
Imprint:	New York : Humana Press, 2014.
Description:	1 online resource (x, 340 pages) : illustrations (some color)
Language:	English
Series:	Methods in Molecular Biology, 1064-3745 ; 1086 Methods in molecular biology (Clifton, N.J.) ; v. 1086.
Subject:	RNA -- Laboratory manuals. Publication Formats Publication Characteristics Laboratory Manuals RNA. Laboratory manuals.
Format:	E-Resource Book
URL for this record:	http://pi.lib.uchicago.edu/1001/cat/bib/11210153

Hidden Bibliographic Details
Other authors / contributors:	Waldsich, Christina, editor.
ISBN:	9781627036672 1627036679 9781627036665 1627036660 9781627036665
Digital file characteristics:	text file PDF
Notes:	Includes index. Includes bibliographical references and index. English. Online resource; title from PDF title page (SpringerLink, viewed October 21, 2013).
Summary:	Focusing on in vitro and intracellular RNA structure formation, RNA Folding: Methods and Protocols provides a comprehensive collection of experimental protocols which are suitable to dissect RNA folding pathways and to characterize the structure of RNA folding intermediates at nucleotide or even atomic resolution. The presented techniques include powerful tools with a long tradition in RNA research as well as more advanced, novel methods, thus the methods span multiple disciplines, including molecular biology, biochemistry, biophysics, and computational biology. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Practical and authoritative, RNA Folding: Methods and Protocols serves as a vital reference for researchers attempting to gain insights into the secrets of this astounding macromolecule.
Other form:	Print version: RNA folding. New York : Humana Press, 2014
Standard no.:	10.1007/978-1-62703-667-2

Table of Contents:

Predicting RNA Structure: Advances and Limitations
Cis-Acting 5' Hammerhead Optimization for In Vitro Transcription of Highly Structured RNAs
RNA Structural Analysis by Enzymatic Digestion
A Mutate-and-Map Protocol for Inferring Base Pairs in Structured RNA
Mapping RNA Structure In Vitro Using Nucleobase-Specific Probes
Massively Parallel RNA Chemical Mapping with a Reduced Bias MAP-Seq Protocol
Probing RNA Folding by Hydroxyl Radical Footprinting
Monitoring Global Structural Changes and Specific Metal Binding Sites in RNA by In-Line Probing and Tb(III) Cleavage
Chemical Probing of RNA in Living Cells
A Chemogenetic Approach to Study the Structural Basis of Protein-Facilitated RNA Folding
Applying UV Crosslinking to Study RNA-Protein Interactions in Multicomponent Ribonucleoprotein Complexes
The Kinetics of Ribozyme Cleavage: A Tool to Analyze RNA Folding as a Function of Catalysis
RNA Catalytic Activity as a Probe of Chaperone-Mediated RNA Folding
Evaluation of RNA Chaperone Activity In Vivo and In Vitro Using Misfolded Group I Ribozymes
RNA Conformational Changes Analyzed by Comparative Gel Electrophoresis
Detecting RNA Tertiary Folding by Sedimentation Velocity Analytical Ultracentrifugation
RNA Folding Dynamics Using Laser Assisted Single-Molecule Refolding
RNA Refolding Studied by Light-Coupled NMR Spectroscopy
Exploring RNA Oligomerization and Ligand Binding by Fluorescence Correlation Spectroscopy and Small Angle X-Ray Scattering.

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