Human retroviruses : methods and protocols /
Saved in:
Imprint: | New York : Humana Press, [2014] ©2014 |
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Description: | 1 online resource (xviii, 345 pages) : illustrations (some color) |
Language: | English |
Series: | Methods in Molecular Biology, 1940-6029 ; Volume No. 1087 Methods in molecular biology (Clifton, N.J.) ; v. 1087. |
Subject: | |
Format: | E-Resource Book |
URL for this record: | http://pi.lib.uchicago.edu/1001/cat/bib/11210653 |
Table of Contents:
- Part I. Mapping the HIV Life Cycle
- Virion Attachment and Entry: HIV gp120 Env Biotinylation, gp120 Env, or Integrin Ligand-Binding Assay / Claudia Cicala and James Arthos
- CryoEM Analysis of Capsid Assembly and Structural Changes Upon Interactions with a Host Restriction Factor, TRIM5[alpha] / Gongpu Zhao and Peijun Zhang
- The Fate of HIV-1 Capsid: A Biochemical Assay for HIV-1 Uncoating / Yang Yang, Jeremy Luban, and Felipe Diaz-Griffero
- The Cyclosporin A Washout Assay to Detect HIV-1 Uncoating in Infected Cells / Amy E. Hulme and Thomas J. Hope
- Imaging HIV-1 Nuclear Pre-integration Complexes / Anna Cereseto and Mauro Giacca
- HIV-1 Reverse Transcription / Andrea Cimarelli and Jean-Luc Darlix
- RNase H: Specificity, Mechanisms of Action, and Antiviral Target / Karin Moelling, Felix Broecker, and John E. Kerrigan
- HIV-1 Chromatin, Transcription, and the Regulatory Protein Tat / Laurence Colin, Eric Verdin, and Carine Van Lint
- HIV-1 Rev Function and RNA Nuclear-Cytoplasmic Export / Alan Cochrane
- HIV-1 Accessory Proteins: Nef / Anke Heigele, Daniel Sauter, Jan Münch, and Frank Kirchhoff
- HIV-1 Accessory Proteins: VpR / Richard Y. Zhao and Michael I. Bukrinsky
- HIV-1 Accessory Proteins: Vpu and Vif / Amy Andrew and Klaus Strebel
- SIVSM/HIV-2 Vpx Proteins: Function and Uses in the Infection of Primary Myeloid Cells / Gregory Berger and Andrea Cimarelli
- Imaging of HIV Assembly and Release / Barbara Müller and Jacomine Krijnse-Locker.
- Part II. Isolation, Co-Receptor Use, and Cell Tropism of HIV-1
- HIV-1 Isolation from Infected Peripheral Blood Mononuclear Cells / Stefania Dispinseri, Elisa Saba, Elisa Vicenzi, Neeltje A. Kootstra, Hanneke Schuitemaker, and Gabriella Scarlatti
- Determination of HIV-1 Co-receptor Usage / Mariangela Cavarelli and Gabriella Scarlatti
- The Macrophage and HIV: Basic Concepts and Methodologies / Suzanne Gartner
- HIV Infection of Dendritic Cells / Najla Nasr, Andrew Harman, Stuart Turville, and Anthony L. Cunningham
- Histocultures (Tissue Explants) in Human Retrovirology / Anush Arakelyan, Wendy Fitzgerald, Jean-Charles Grivel, Christophe Vanpouille, and Leonid Margolis.
- Part III. In Vivo Quantification of HIV-1
- Single-Copy Quantification of HIV-1 in Clinical Samples / Ann Wiegand and Frank Maldarelli
- Quantification of Total HIV1-DNA in Peripheral Blood Mononuclear Cells / Christine Rouzioux, Adeline Mélard, and Véronique Avéttand-Fénoël.
- Part IV. Biotechnological Aspects of HIV-1
- HIV-1-Based Lentiviral Vectors / Ying Poi Liu and Ben Berkhout
- Quantification of miRNA by Poly(A)-RT-qPCR Arrays and Verification of Target Sites in HIV-1 Using a One-LTR Infectious Molecular Clone / Zachary A. Klase, Laurent Houzet, and Kuan-Teh Jeang.
- Part V. HTLVS
- Investigating Human T Cell Lymphotropic Retrovirus (HTLV) Tax Function with Molecular and Immunophenotypic Techniques / Greta Forlani, Roberto S. Accolla, and Giovanna Tosi
- Proviral Load Determination of HTLV-1 and HTLV-2 in Patients' Peripheral Blood Mononuclear Cells by Real-Time PCR / Claudio Casoli, Elisabetta Pilotti, and Umberto Bertazzoni
- Quantitative Analysis of Human T-Lymphotropic Virus Type 1 (HTLV-1) Gene Expression Using Nucleo-Cytoplasmic Fractionation and Splice Junction-Specific Real-Time RT-PCR (qRT-PCR) / Ilaria Cavallari, Francesca Rende, and Vincenzo Ciminale.