Human retroviruses : methods and protocols /

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Bibliographic Details
Imprint:	New York : Humana Press, [2014] ©2014
Description:	1 online resource (xviii, 345 pages) : illustrations (some color)
Language:	English
Series:	Methods in Molecular Biology, 1940-6029 ; Volume No. 1087 Methods in molecular biology (Clifton, N.J.) ; v. 1087.
Subject:	Retroviruses -- Laboratory manuals. HIV (Viruses) -- Laboratory manuals. HTLV (Viruses) -- Laboratory manuals. HIV (Viruses) HTLV (Viruses) Retroviruses. Lentiviruses, Primate. RNA Viruses. Oncogenic Viruses. Publication Formats Vertebrate Viruses Lentivirus. Viruses. Publication Characteristics Organisms HIV. Retroviridae. Laboratory Manuals Laboratory manuals.
Format:	E-Resource Book
URL for this record:	http://pi.lib.uchicago.edu/1001/cat/bib/11210653

Hidden Bibliographic Details
Other authors / contributors:	Vicenzi, Elisa, editor Poli, Guido, editor
ISBN:	9781627036702 1627036709 1627036695 9781627036696 9781627036696
Digital file characteristics:	text file PDF
Notes:	Includes bibliographical references and index. English. Online resource; title from PDF title page (Springer Protocols, viewed January 30, 2014).
Summary:	"Human Retroviruses: Methods and Protocols collects key experimental protocols that have provided the basis of the major discoveries of the field. Split into five sections, this detailed volume covers mapping of the HIV life cycle, isolation, co-receptor use, and cell tropism of HIV-1, in vivo quantification of HIV-1, biological aspects of HIV-1, as well as HTLVs. Some articles explore assay and function of accessory genes, largely involving the interface between retroviral and host factors, the extracellular role of Tat and Tax, resembling the function of cytokines, and the biotechnological exploitation of HIV as lentiviral vector to carry foreign genes with therapeutic value. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Comprehensive and authoritative, Human Retroviruses: Methods and Protocols provides state-of-art methodological protocols from world leaders in human retrovirology, essential for any lab working this vital field."--Publisher's description.
Other form:	Print version: Human retroviruses. New York : Humana Press, [2014] 1627036695
Standard no.:	10.1007/978-1-62703-670-2

Table of Contents:

Part I. Mapping the HIV Life Cycle
Virion Attachment and Entry: HIV gp120 Env Biotinylation, gp120 Env, or Integrin Ligand-Binding Assay / Claudia Cicala and James Arthos
CryoEM Analysis of Capsid Assembly and Structural Changes Upon Interactions with a Host Restriction Factor, TRIM5[alpha] / Gongpu Zhao and Peijun Zhang
The Fate of HIV-1 Capsid: A Biochemical Assay for HIV-1 Uncoating / Yang Yang, Jeremy Luban, and Felipe Diaz-Griffero
The Cyclosporin A Washout Assay to Detect HIV-1 Uncoating in Infected Cells / Amy E. Hulme and Thomas J. Hope
Imaging HIV-1 Nuclear Pre-integration Complexes / Anna Cereseto and Mauro Giacca
HIV-1 Reverse Transcription / Andrea Cimarelli and Jean-Luc Darlix
RNase H: Specificity, Mechanisms of Action, and Antiviral Target / Karin Moelling, Felix Broecker, and John E. Kerrigan
HIV-1 Chromatin, Transcription, and the Regulatory Protein Tat / Laurence Colin, Eric Verdin, and Carine Van Lint
HIV-1 Rev Function and RNA Nuclear-Cytoplasmic Export / Alan Cochrane
HIV-1 Accessory Proteins: Nef / Anke Heigele, Daniel Sauter, Jan Münch, and Frank Kirchhoff
HIV-1 Accessory Proteins: VpR / Richard Y. Zhao and Michael I. Bukrinsky
HIV-1 Accessory Proteins: Vpu and Vif / Amy Andrew and Klaus Strebel
SIVSM/HIV-2 Vpx Proteins: Function and Uses in the Infection of Primary Myeloid Cells / Gregory Berger and Andrea Cimarelli
Imaging of HIV Assembly and Release / Barbara Müller and Jacomine Krijnse-Locker.
Part II. Isolation, Co-Receptor Use, and Cell Tropism of HIV-1
HIV-1 Isolation from Infected Peripheral Blood Mononuclear Cells / Stefania Dispinseri, Elisa Saba, Elisa Vicenzi, Neeltje A. Kootstra, Hanneke Schuitemaker, and Gabriella Scarlatti
Determination of HIV-1 Co-receptor Usage / Mariangela Cavarelli and Gabriella Scarlatti
The Macrophage and HIV: Basic Concepts and Methodologies / Suzanne Gartner
HIV Infection of Dendritic Cells / Najla Nasr, Andrew Harman, Stuart Turville, and Anthony L. Cunningham
Histocultures (Tissue Explants) in Human Retrovirology / Anush Arakelyan, Wendy Fitzgerald, Jean-Charles Grivel, Christophe Vanpouille, and Leonid Margolis.
Part III. In Vivo Quantification of HIV-1
Single-Copy Quantification of HIV-1 in Clinical Samples / Ann Wiegand and Frank Maldarelli
Quantification of Total HIV1-DNA in Peripheral Blood Mononuclear Cells / Christine Rouzioux, Adeline Mélard, and Véronique Avéttand-Fénoël.
Part IV. Biotechnological Aspects of HIV-1
HIV-1-Based Lentiviral Vectors / Ying Poi Liu and Ben Berkhout
Quantification of miRNA by Poly(A)-RT-qPCR Arrays and Verification of Target Sites in HIV-1 Using a One-LTR Infectious Molecular Clone / Zachary A. Klase, Laurent Houzet, and Kuan-Teh Jeang.
Part V. HTLVS
Investigating Human T Cell Lymphotropic Retrovirus (HTLV) Tax Function with Molecular and Immunophenotypic Techniques / Greta Forlani, Roberto S. Accolla, and Giovanna Tosi
Proviral Load Determination of HTLV-1 and HTLV-2 in Patients' Peripheral Blood Mononuclear Cells by Real-Time PCR / Claudio Casoli, Elisabetta Pilotti, and Umberto Bertazzoni
Quantitative Analysis of Human T-Lymphotropic Virus Type 1 (HTLV-1) Gene Expression Using Nucleo-Cytoplasmic Fractionation and Splice Junction-Specific Real-Time RT-PCR (qRT-PCR) / Ilaria Cavallari, Francesca Rende, and Vincenzo Ciminale.

Human retroviruses : methods and protocols /

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