Therapeutic applications of ribozymes and riboswitches : methods and protocols /

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Bibliographic Details
Imprint:	New York : Humana Press, [2014] ©2014
Description:	1 online resource (xi, 278 pages) : illustrations (some color)
Language:	English
Series:	Methods in Molecular Biology, 1940-6029 ; 1103 Methods in molecular biology (Clifton, N.J.) ; v. 1103.
Subject:	Catalytic RNA -- Laboratory manuals. Riboswitches -- Laboratory manuals. RNA, Messenger. Publication Formats RNA, Untranslated. Enzymes. Regulatory Sequences, Ribonucleic Acid. RNA. Publication Characteristics Untranslated Regions. Enzymes and Coenzymes Regulatory Sequences, Nucleic Acid. Nucleic Acids. Chemicals and Drugs Base Sequence. Molecular Structure. Nucleic Acids, Nucleotides, and Nucleosides Genetic Structures. Genetic Phenomena. Biochemical Phenomena. Chemical Phenomena. Phenomena and Processes RNA, Catalytic. Riboswitch. Laboratory Manuals Catalytic RNA. Riboswitches. Laboratory Manual. Laboratory manuals.
Format:	E-Resource Book
URL for this record:	http://pi.lib.uchicago.edu/1001/cat/bib/11219868

Hidden Bibliographic Details
Other authors / contributors:	Lafontaine, Daniel, editor. Dubé, Audrey (Biologist), editor.
ISBN:	9781627037303 1627037306 1627037292 9781627037297 9781627037297
Digital file characteristics:	text file PDF
Notes:	Includes bibliographical references and index. English. Online resource; title from PDF title page (SpringerLink, viewed December 10, 2013).
Summary:	In Therapeutic Applications of Ribozymes and Riboswitches: Methods and Protocols, expert researchers in the field provide a complete overview of protocols used in the development of RNA molecule as drugs and drug target. These include methods and protocols on recent and precise RNA molecule approaches or studies in the development of an RNA therapeutic tool. We are convinced that these methods will help researchers from various domains of life sciences, including clinicians, biochemists and virologists. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Therapeutic Applications of Ribozymes and Riboswitches: Methods and Protocols seeks to aid scientists in the further development of new therapeutic applications implicating RNA molecules or directly targeting harmful RNA.
Other form:	Print version: Therapeutic applications of ribozymes and riboswitches. New York : Humana Press, [2014]
Standard no.:	10.1007/978-1-62703-730-3

Table of Contents:

Identification of regulatory RNA in bacterial genomes by genome-scale mapping of transcription start sites
Screening inhibitory potential of anti-HIV RT RNA aptamers
Design and evaluation of clinically relevant SOFA-HDV ribozymes targeting HIV RNA
Directing RNase P-mediated cleavage of target mRNAs by engineered external guide sequences in cultured cells
Design and analysis of hammerhead ribozyme activity against an artificial gene target
Knockdown strategies for the study of proprotein convertases and proliferation in prostate cancer cells
Use of tumor-targeting trans-splicing ribozyme for cancer treatment
Characterization of hairpin ribozyme reactions
Finding instances of riboswitches and ribozymes by homology search of structured RNA with infernal
Structure-based virtual screening for the identification of RNA- binding ligands
Probing riboswitch binding sites with molecular docking, focused libraries, and in-line probing assays
Discovery of small molecule modifiers of micrornas for the treatment of HCV infection
Bacterial flavin mononucleotide riboswitches as targets for flavin analogs
Construction and application of riboswitch-based sensors that detect metabolites within bacterial cells
Screening assays to identify artificial glmS ribozyme activators
Analysis of riboswitch structure and ligand binding using small-angle X-ray scattering (SAXS)
Use of SHAPE to select 2AP substitution sites for RNA-ligand interactions and dynamics studies
Cell internalization SELEX: In vitro selection for molecules that internalize into cells
DNA electronic switches based on analyte-responsive aptamers.

Therapeutic applications of ribozymes and riboswitches : methods and protocols /

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