Yeast metabolic engineering : methods and protocols /

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Bibliographic Details
Imprint:	New York, NY : Humana Press, [2014] ©2014
Description:	1 online resource (x, 316 pages) : illustrations (some color)
Language:	English
Series:	Methods in Molecular Biology, 1940-6029 ; 1152 Methods in molecular biology (Clifton, N.J.) ; v. 1152.
Subject:	Yeast -- Laboratory manuals. Yeast fungi -- Biotechnology -- Laboratory manuals. Metabolic Engineering Yeasts -- metabolism Yeast Yeast fungi -- Biotechnology Laboratory Manual Laboratory manuals Laboratory manuals.
Format:	E-Resource Book
URL for this record:	http://pi.lib.uchicago.edu/1001/cat/bib/11225673

Hidden Bibliographic Details
Other authors / contributors:	Mapelli, Valeria, editor.
ISBN:	9781493905638 1493905635 1493905627 9781493905621 9781493905621
Notes:	Includes bibliographical references and index. Online resource; title from PDF title page (SpringerLink, viewed April 25, 2014).
Summary:	Yeast Metabolic Engineering: Methods and Protocols provides the widely established basic tools used in yeast metabolic engineering, while describing in deeper detail novel and innovative methods that have valuable potential to improve metabolic engineering strategies in industrial biotechnology applications. Beginning with an extensive section on molecular tools and technology for yeast engineering, this detailed volume is not limited to methods for Saccharomyces cerevisiae, but describes tools and protocols for engineering other yeasts of biotechnological interest, such as Pichia pastoris, Hansenula polymorpha, and Zygosaccharomyces bailii. Tools and technologies for the investigation and determination of yeast metabolic features are described in detail, as well as metabolic models and their application for yeast metabolic engineering, while a chapter describing patenting and regulations with a special glance at yeast biotechnology closes the volume. Written in the highly successful Methods in Molecular Biology series format, most chapters include an introduction to their respective topic, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Comprehensive and authoritative, Yeast Metabolic Engineering: Methods and Protocols aims to familiarize researchers with the current state of these vital and increasingly useful technologies.
Other form:	Print version: Yeast metabolic engineering. New York : Humana Press, [2014] 9781493905621
Standard no.:	10.1007/978-1-4939-0563-8

Table of Contents:

An overview on selection marker genes for transformation of Saccharomyces cerevisiae
Natural and modified promoters for tailored metabolic engineering of the yeast Saccharomyces cerevisiae
Tools for genetic engineering of the yeast Hansenula polymorpha
Molecular tools and protocols for engineering the acid-tolerant yeast Zygosaccharomyces bailii as a potential cell factory
Strains and molecular tools for recombinant protein production in Pichia pastoris
Methods for efficient high-throughput screening of protein expression in recombinant pichia pastoris strains
Synthetic RNA switches for yeast metabolic engineering: Screening recombinant enzyme libraries
Generation of arming yeasts with active proteins and peptides via cell surface display system: Cell surface engineering, bio-arming technology
Genetic engineering of industrial Saccharomyces cerevisiae strains using a selection/counter-selection approach
Evolutionary engineering of yeast
Determination of a dynamic feeding strategy for recombinant pichia pastoris strains
Yeast metabolomics: Sample preparation for a GC/MS based analysis
13CC-based metabolic flux analysis in yeast: The Pichia pastoris case
Pathway activity profiling (PAPi): A tool for metabolic pathway analysis
QTL mapping by pooled-segregant whole-genome sequencing in yeast
Genome-scale metabolic models of yeast, methods for their reconstruction, and other applications
Model-guided identification of gene deletion targets for metabolic engineering in Saccharomyces cerevisiae
Patents: A tool to bring innovation from the lab bench to the marketplace.

Yeast metabolic engineering : methods and protocols /

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