T-cell trafficking : methods and protocols /

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Bibliographic Details
Imprint:Totowa, N.J. : Humana ; London : Springer [distributor], ©2010.
Description:1 online resource
Language:English
Series:Methods in molecular biology ; 616
Methods in molecular biology (Clifton, N.J.) ; 616.
Subject:
Format: E-Resource Book
URL for this record:http://pi.lib.uchicago.edu/1001/cat/bib/11228143
Hidden Bibliographic Details
Other authors / contributors:Marelli-Berg, Federica M.
Nourshargh, Sussan.
ISBN:9781607614616
1607614618
9781607614609
160761460X
Digital file characteristics:text file
PDF
Notes:Includes bibliographical references and index.
English.
Print version record.
Summary:In the last decade, a large number of major discoveries have shed light on the molecular mechanisms of lymphocyte migration and the anatomy of immune responses. In T-Cell Trafficking: Methods and Protocols, expert researchers explore how the development of novel and cutting-edge techniques, particularly in the field of real-time imaging and genetic manipulation, have led to an increased understanding of lymphocyte trafficking. Written by internationally recognized experts in their respective fields, chapters provide state-of-the-art protocols to study lymphocyte migration and T-cell: endothelial cell interactions in vitro, address various approaches used for direct visualization of the development of the lymphoid system, lymphocyte recirculation, and effector responses in experimental models in vivo, and explore lymphocyte migration and inflammation in the human system. Composed in the highly successful Methods in Molecular Biology series format, each chapter contains a brief introduction, step-by-step methods, a list of necessary materials, and a Notes section which shares tips on troubleshooting and avoiding known pitfalls. Innovative and highly practical, T-Cell Trafficking: Methods and Protocols is an essential manual for newcomers in this ever-expanding and exciting area of research, as well as a valuable addition to more specialized laboratories.
Other form:Print version: T-cell trafficking. Totowa, N.J. : Humana ; London : Springer [distributor], ©2010 9781607614609 160761460X
Standard no.:10.1007/978-1-60761-461-6

MARC

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490 1 |a Methods in molecular biology ;  |v 616 
505 0 |a How T cells find their way around / Alf Hamann -- Live imaging of leukocyte-endothelium interactions / Olga Barreiro, Francisco Sánchez-Madrid, and María Yáñez-Mó -- Leucocyte adhesion under haemodynamic flow conditions / Charlotte Lawson, Marlene Rose, and Sabine Wolf -- Influence of stromal cells on lymphocyte adhesion and migration on endothelial cells / Helen M. McGettrick [and others] -- Discriminating between the paracellular and transcellular routes of diapedesis / Jaime Millán, Eva Cernuda-Morollón, and Severine Gharbi -- Monitoring RhoGTPase activity in lymphocytes / Marouan Zarrouk, David Killock, and Aleksandar Ivetic -- Visualisation of signalling in immune cells / Leo M. Carlin [and others] -- Methods for quantitation of leukocyte chemotaxis and fugetaxis / Fabrizio Vianello, Elda Righi, and Mark C. Poznansky -- Analysis of CXCR3 and atypical variant expression and signalling in human T lymphocytes / Anna Korniejewska, Malcolm Watson, and Stephen Ward -- Transfection of indoleamine 2,3 dioxygenase in primary endothelial cells / Petros X.E. Mouratidis and Andrew J.T. George -- Visualisation of lymphoid organ development / Henrique Veiga-Fernandes [and others] -- Single-cell analysis of cytotoxic T cell function by intravital multiphoton microscopy / Thorsten R. Mempel -- Imaging interactions between the immune and cardiovascular systems in vivo by multiphoton microscopy / Owain R. Millington [and others] -- Applying an adaptive watershed to the tissue cell quantification during T-cell migration and embryonic development / D. Zhu [and others] -- Identifying homing interactions in T-cell traffic in human disease / Patricia F. Lalor, Stuart M. Curbishley, and David H. Adams -- Tracking antigen-experienced effector T cells in vitro and in vivo / Claire L. Gorman [and others] -- Preclinical testing of strategies for therapeutic targeting of human T-cell trafficking in vivo / Caroline Coisne and Britta Engelhardt. 
588 0 |a Print version record. 
504 |a Includes bibliographical references and index. 
520 |a In the last decade, a large number of major discoveries have shed light on the molecular mechanisms of lymphocyte migration and the anatomy of immune responses. In T-Cell Trafficking: Methods and Protocols, expert researchers explore how the development of novel and cutting-edge techniques, particularly in the field of real-time imaging and genetic manipulation, have led to an increased understanding of lymphocyte trafficking. Written by internationally recognized experts in their respective fields, chapters provide state-of-the-art protocols to study lymphocyte migration and T-cell: endothelial cell interactions in vitro, address various approaches used for direct visualization of the development of the lymphoid system, lymphocyte recirculation, and effector responses in experimental models in vivo, and explore lymphocyte migration and inflammation in the human system. Composed in the highly successful Methods in Molecular Biology series format, each chapter contains a brief introduction, step-by-step methods, a list of necessary materials, and a Notes section which shares tips on troubleshooting and avoiding known pitfalls. Innovative and highly practical, T-Cell Trafficking: Methods and Protocols is an essential manual for newcomers in this ever-expanding and exciting area of research, as well as a valuable addition to more specialized laboratories. 
546 |a English. 
650 0 |a T cells.  |0 http://id.loc.gov/authorities/subjects/sh85131766 
650 0 |a Cell differentiation  |x Molecular aspects.  |0 http://id.loc.gov/authorities/subjects/sh91002598 
650 0 |a Lymphocytes  |x Metabolism  |x Research. 
650 0 |a Cells  |x Motility  |x Research. 
650 2 |a Lymphocytes. 
650 2 |a Biological Science Disciplines. 
650 2 |a Cell Physiological Phenomena. 
650 2 |a Locomotion. 
650 2 |a Natural Science Disciplines. 
650 2 |a Movement. 
650 2 |a Leukocytes, Mononuclear. 
650 2 |a T-Lymphocytes. 
650 2 |a Cell Movement. 
650 2 |a Physiology. 
650 2 |a Phenomena and Processes 
650 2 |a Physiological Phenomena. 
650 2 |a Disciplines and Occupations 
650 2 |a Leukocytes. 
650 2 |a Blood Cells. 
650 2 |a Immune System. 
650 2 |a Blood. 
650 2 |a Hemic and Immune Systems 
650 2 |a Cells. 
650 2 |a Anatomy. 
650 7 |a Cell differentiation  |x Molecular aspects.  |2 fast  |0 (OCoLC)fst00850184 
650 7 |a T cells.  |2 fast  |0 (OCoLC)fst01141544 
653 0 0 |a t cellen 
653 0 0 |a t lymphocytes 
653 0 0 |a immunologie 
653 0 0 |a immunology 
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700 1 |a Nourshargh, Sussan.  |0 http://id.loc.gov/authorities/names/nb2010028412 
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