Pseudomonas methods and protocols /

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Bibliographic Details
Imprint:	New York, NY : Humana Press, 2014.
Description:	1 online resource (xviii, 816 pages) : illustrations (some color)
Language:	English
Series:	Methods in Molecular Biology, 1064-3745 ; 1149 Methods in molecular biology (Clifton, N.J.) ; v. 1149.
Subject:	Pseudomonas -- Laboratory manuals. Pseudomonas aeruginosa -- genetics. Pseudomonas. Laboratory Manual. Laboratory manuals.
Format:	E-Resource Book
URL for this record:	http://pi.lib.uchicago.edu/1001/cat/bib/11230724

Hidden Bibliographic Details
Other authors / contributors:	Filloux, Alain, editor. Ramos, Juan-Luis, 1956- editor.
ISBN:	9781493904730 1493904736 1493904728 9781493904723 9781493904723
Notes:	Includes bibliographical references and index. Online resource; title from PDF title page (SpringerLink, viewed June 6, 2014).
Summary:	"In Pseudomonas aeruginosa, expert researchers in the field detail many of the methods which are now commonly used to study this fascinating microorganism. Chapters include microbiological methods to high-throughput molecular techniques that have been developed over the last decade. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Pseudomonas aeruginosa aids in the continuing study of new and cutting edge findings."--Back cover.
Other form:	Print version: Pseudomonas methods and protocols. New York, NY : Humana Press, 2014
Standard no.:	10.1007/978-1-4939-0473-0

Table of Contents:

Gene transfer : transduction / Emanuela Frangipani
Gene transfer : transformation/electroporation / Frédéric Cadoret, Chantal Soscia, and Romé Voulhoux
Gene transfer : conjugation / Thibault G. Sana, Aurélie Laubier, and Sophie Bleves
Pseudomonas bacteriophage isolation and production / Joana Azeredo, Sanna Sillankorva, and Diana P. Pires
Genotyping methods / Burkhard Tümmler
Drug susceptibility testing by dilution methods / Katy Jeannot and Patrick Plésiat
Plate-based assay for swimming motility in Pseudomonas aeruginosa / Dae-Gon Ha, Sherry L. Kuchma, and George A. O'Toole
Plate-based assay for swarming motility in Pseudomonas aeruginosa / Dae-Gon Ha, Sherry L. Kuchma, and George A. O'Toole
Motility assay : twitching motility / Lynne Turnbull and Cynthia B. Whitchurch
Qualitative and quantitative assays for flagellum- mediated chemotaxis / José Antonio Reyes Darias [and others]
Microscopic analysis : morphotypes and cellular appendages / Olga Zaborina, John Alverdy, Megha Shah, and Yimei Chen
Determination of lipolytic enzyme activities / Karl-Erich Jaeger and Filip Kovacic
Elastinolytic and Proteolytic Enzymes / Efrat Kessler and Mary Safrin
In vitro assays to monitor the activity of Pseudomonas aeruginosa type III secreted proteins / Stephanie L. Rolsma and Dara W. Frank
Cell fractionation / Bérengère Ize, Véronique Viarre, and Romé Voulhoux
Characterization of molecular interactions using isothermal titration calorimetry / Tino Krell [and others]
Proteomic analysis / Pascal Cosette and Thierry Jouenne
Membrane proteomics of Pseudomonas aeruginosa / Zofia Magnowska, Isabel Hartmann, Lothar Jänsch, and Dieter Jahn
Construction of Pseudomonas aeruginosa two-hybrid libraries for high-throughput assays / Sophie de Bentzmann and Christophe Bordi
Biosensors for qualitative and semiquantitative analysis of quorum sensing signal molecules / Matthew Fletcher [and others]
LC-MS/MS quantitative analysis of quorum sensing signal molecules / Catharine A. Ortori [and others]
LC/MS/MS-based quantitative assay for the secondary messenger molecule, c-di-GMP / Yasuhiko Irie and Matthew R. Parsek
Metabolic footprinting : extracellular metabolomic analysis / Volker Behrends, Huw D. Williams, and Jacob G. Bundy
Pyoverdine and pochelin measurements / Françoise Hoegy, Gaetan L.A. Mislin, and Isabelle J. Schalk
Measurement of phenazines in bacterial cultures / Suzanne E. Kern and Dianne K. Newman
Extraction and measurement of NAD(P)+ and NAD(P)H / Suzanne E. Kern, Alexa Price-Whelan, and Dianne K. Newman
Cyanide measurements in bacterial culture and sputum / Chandrika Goh Nair, Ben Ryall, and Huw D. Williams
Monitoring iron uptake by siderophores / Françoise Hoegy and Isabelle J. Schalk
Exopolysaccharide quantification / Irina Sadovskaya
Liquid chromatography/mass spectrometry for the identification and quantification of rhamnolipids / Ahmad Mohammad Abdel-Mawgoud, François Lépine, and Eric Déziel
LPS quantitation procedures/ Joseph S. Lam, Erin M. Anderson, and Youai Hao
Monitoring lectin interactions with carbohydrates / Sophie de Bentzmann, Annabelle Varrot, and Anne Imberty
Mining the pseudomonas genome / Geoffrey L. Winsor and Fiona S.L. Brinkman
Identification of bacterial small RNAs by RNA sequencing / María Gómez-Lozano [and others]
Gene amplification and qRT-PCR / Cerith Jones and Alain Filloux
Standard european vector architecture (SEVA) plasmid toolkit / Gonzalo Durante-Rodríguez, Víctor de Lorenzo, and Esteban Martínez-García
Chromosomal integration of transcriptional fusions / Rafael Silva-Rocha and Víctor de Lorenzo
Method to capture large DNA fragments from genomic DNA / Geneviève Ball, Alain Filloux, and Romé Voulhoux
Transposon mutagenesis / Hemantha D. Kulasekara
Site-directed mutagenesis and gene deletion using reverse genetics / Daniela Muhl and Alain Filloux
Signature-tagged mutagenesis / Irena Kukavica-Ibrulj and Roger C. Levesque
Construction of a Pseudomonas aeruginosa genomic DNA library / Christophe Bordi
Strategy for genome sequencing analysis and assembly for comparative genomics of Pseudomonas Genomes / Julie Jeukens [and others]
Promoter fusions with optical outputs in individual cells and in populations / Ilaria Benedetti and Victor de Lorenzo
Chromatin immunoprecipitation for ChIP-chip and ChIP-seq / Sebastian Schulz and Susanne Häussler
Transcriptional analysis of Pseudomonas aeruginosa infected Caenorhabditis elegans / Ashleigh MacKenzie [and others]
Methods for studying biofilm formation : flow cells and confocal laser scanning microscopy / Tim Tolker-Nielsen and Claus Sternberg
Biofilm formation in the 96-well microtiter plate / Barbara M. Coffey and Gregory G. Anderson
Methods for studying biofilm dispersal in Pseudomonas aeruginosa / Nicolas Barraud [and others]
Pseudomonas aeruginosa PA14 pathogenesis in Caenorhabditis elegans / Natalia V. Kirienko [and others]
Assessing Pseudomonas aeruginosa virulence using a nonmammalian host : Dictyostelium discoideum / Geneviève Filion and Steve J. Charette
Assessing pseudomonas virulence with nonmammalian host : Galleria mellonella / Gudrun Koch [and others]
Assessing pseudomonas virulence with the nonmammalian host model : Arabidopsis thaliana / Regina L. Baldini, Melissa Starkey, and Laurence G. Rahme
Assessing Pseudomonas aeruginosa persister/antibiotic tolerant cells / Ronen Hazan [and others]
Assessing pseudomonas virulence with nonmammalian host : zebrafish / María A. Llamas and Astrid M. van der Sar
Assessing pseudomonas virulence with a nonmammalian host : Drosophila melanogaster / Samantha Haller, Stefanie Limmer, and Dominique Ferrandon
Assessing pseudomonas virulence using host cells / Iwona Bucior, Cindy Tran, and Joanne Engel
Assessing Pseudomonas aeruginosa virulence and the host response using Murine models of acute and chronic lung infection / Irena Kukavica-Ibrulj [and others] Bragonzi
Assessing pseudomonas virulence using mammalian models : acute infection model / Antje Munder and Burkhard Tümmler
Burn mouse models / Henrik Calum, Niels Høiby, and Claus Moser.

Pseudomonas methods and protocols /

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