The nucleus /

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Bibliographic Details
Edition:	Second edition.
Imprint:	New York : Humana Press, [2015] ©2015
Description:	1 online resource (xi, 237 pages) : illustrations (some color)
Language:	English
Series:	Methods in molecular biology, 1064-3745 ; 1228 Methods in molecular biology (Clifton, N.J.) ; v. 1228.
Subject:	Cell nuclei -- Research -- Laboratory manuals. Cell Nucleus. Cell Nucleus Structures. Cytological Techniques. Laboratory manuals.
Format:	E-Resource Book
URL for this record:	http://pi.lib.uchicago.edu/1001/cat/bib/11237205

Hidden Bibliographic Details
Other authors / contributors:	Hancock, Ronald, editor
ISBN:	9781493916801 1493916807 1493916793 9781493916795 9781493916795
Digital file characteristics:	text file PDF
Notes:	Includes bibliographical references and indexes.
Summary:	This volume presents detailed, recently-developed protocols ranging from isolation of nuclei to purification of chromatin regions containing single genes, with a particular focus on some less well-explored aspects of the nucleus. The methods described include new strategies for isolation of nuclei, for purification of cell type-specific nuclei from a mixture, and for rapid isolation and fractionation of nucleoli. For gene delivery into and expression in nuclei, a novel gentle approach using gold nanowires is presented. As the concentration and localization of water and ions are crucial for macromolecular interactions in the nucleus, a new approach to measure these parameters by correlative optical and cryo-electron microscopy is described. The Nucleus, Second Edition presents methods and software for high-throughput quantitative analysis of 3D fluorescence microscopy images, for quantification of the formation of amyloid fibrils in the nucleus, and for quantitative analysis of chromosome territory localization. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Â Authoritative and easily accessible, The Nucleus, Second Edition seeks to serve both professionals and novices with its well-honed methods for the study of the nucleus.
Other form:	Print version: Nucleus. Second edition. New York : Humana Press, [2015]
Standard no.:	10.1007/978-1-4939-1680-1

Table of Contents:

Cell type-specific affinity purification of nuclei for chromatin profiling in whole animals / Florian A. Steiner and Steven Henikoff
Lysis gradient centrifugation : a flexible method for the isolation of nuclei from primary cells / Karl Katholnig [and others]
Isolation of nuclei in media containing an inert polymer to mimic the crowded cytoplasm / Ronald Hancock and Yasmina Hadj-Sahraoui
New rapid method for isolating nucleoli / Zhou Fang Li and Yun Wah Lam
Sequential recovery of macromolecular components of the nucleolus / Baoyan Bai and Marikki Laiho
Au nanoinjectors for electrotriggered gene delivery into the cell nucleus / Mijeong Kang and Bongsoo Kim
Improving chromatin immunoprecipitation (ChIP) by suppression of method-induced DNA-damage signaling / Sascha Beneke
Purification of specific chromatin loci for proteomic analysis / Stephanie D. Byrum, Sean D. Taverna, and Alan J. Tackett-- Chromatin structure analysis of single gene molecules by psoralen cross-linking and electron microscopy / Christopher R. Brown [and others]
Purification of proteins on newly synthesized DNA using iPOND / Huzefa Dungrawala and David Cortez
Applying the ribopuromycylation method to detect nuclear translation / Alexandre David and Jonathan W. Yewdell
Targeted nano analysis of water and ions in the nucleus using cryo-correlative microscopy / Frédérique Nolin [and others]
Redox-sensitive yellow fluorescent protein sensor for monitoring nuclear glutathione redox dynamics / Agata Banach-Latapy, Michèle Dardalhon, and Meng-Er Huang
Determination of the dissociation constant of the NF[Kappa]B p50/p65 heterodimer in living cells using fluorescence cross-correlation spectroscopy / Manisha Tiwari and Masataka Kinjo
Imaging and quantification of amyloid fibrillation in the cell nucleus / Florian Arnhold, Andrea Scharf, and Anna von Mikecz
Analysis of nuclear organization with TANGO, software for high-throughput quantitative analysis of 3D fluorescence microscopy images / Jean Ollion [and others]
Quantitative analysis of chromosome localization in the nucleus / Sandeep Chakraborty [and others].

The nucleus /

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