Plant chromosome engineering /

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Bibliographic Details
Imprint:	New York, N.Y. : Humana Press, ©2011.
Description:	1 online resource (xi, 329 pages) : illustrations (some color)
Language:	English
Series:	Methods in molecular biology, 1940-6029 ; v. 701 Springer protocols Methods in molecular biology (Clifton, N.J.) ; volume 701. Springer protocols (Series)
Subject:	Plant chromosomes. Chromosomes, Artificial. Chromosomes, Plant -- genetics. Plant chromosomes. Laboratory Manual.
Format:	E-Resource Book
URL for this record:	http://pi.lib.uchicago.edu/1001/cat/bib/11244959

Hidden Bibliographic Details
Other authors / contributors:	Birchler, James A. (James Arthur), 1950-
ISBN:	9781617379574 1617379573 9781617379567 1617379565
Notes:	Includes bibliographical references and index. Print version record.
Summary:	With an increasing human population and a decreasing amount of arable land, creative improvements in agriculture will be a necessity in the coming decades to maintain or improve the standard of living. In Plant Chromosome Engineering: Methods and Protocols, expert researchers present techniques for the modification of crops and other plant species in order to achieve the goal of developing the much needed novel approaches to the production of food, feed, fuel, fiber, and pharmaceuticals. This volume examines vital topics such as transformation procedures, chromosome painting, production of engineered minichromosomes, gene targeting and mutagenesis, site specific integration, gene silencing, protein expression, chromosome sorting and analysis, protocols for generating chromosomal rearrangements, enhancer trapping, and means of studying chromosomes in vivo. As a part of the highly successful Methods in Molecular Biology™ series, the methodological chapters include brief introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and professional tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Plant Chromosome Engineering: Methods and Protocols highlights the spectrum of tools currently available for modifying plant genomes and chromosomes and provides the foundation for crucial future developments.
Other form:	Print version: Plant chromosome engineering. New York, N.Y. : Humana Press, ©2011 9781617379574

Table of Contents:

Recent advances in plant transformation
Engineering the plastid genome of nicotiana sylvestris, a diploid model species for plastid genetics
Homologous recombination in plants : An antireview
Chromosome painting for plant biotechnology
Plant B chromosomes
Telomere truncation in plants
Engineered plant minichromosomes
Method for bxb1-mediated site-specific integration in planta
Targeted mutagenesis in arabidopsis using zinc-finger nucleases
Vectors and methods for hairpin RNA and artificial microRNA-mediated gene silencing in plants
Recombinant protein expression in nicotiana
Chromosome analysis and sorting using flow cytometry
Super-stretched pachytene chromosomes for plant molecular cytogenetic mapping
Cytological dissection of the triticeae chromosomes by the gametocidal system
Development and use of oat-maize chromosome additions and radiation hybrids
Enhancer trapping in plants
Chromatin beacons : Global sampling of chromatin physical properties using chromatin charting lines
Transposable elements as catalysts for chromosome rearrangements.

Plant chromosome engineering /

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