Protein arginylation : methods and protocols /

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Bibliographic Details
Imprint:	New York, NY : Humana Press, [2015] ©2015
Description:	1 online resource (ix, 149 pages) : illustrations (some color)
Language:	English
Series:	Methods in molecular biology, 1940-6029 ; v. 1337 Methods in molecular biology (Clifton, N.J.) ; volume 1337.
Subject:	Proteins -- Laboratory manuals. Proteins. Proteins Proteins Laboratory Manual Laboratory manuals Laboratory manuals.
Format:	E-Resource Book
URL for this record:	http://pi.lib.uchicago.edu/1001/cat/bib/11247743

Hidden Bibliographic Details
Other authors / contributors:	Kashina, A., editor.
ISBN:	9781493929351 1493929356 1493929348 9781493929344 9781493929344
Notes:	Includes bibliographical references and index. Online resource; title from PDF title page (SpringerLink, viewed August 28, 2015).
Summary:	"This volume presents a comprehensive overview of all the existing methods on analysing protein arginylation, from the early methods utilizing crude protein preparations and whole-cell assays to the latest advanced methods involving recombinant protein techniques, antibodies, high precision mass spectrometry, and chemical probes. This book also includes essays from the founders of the field, who originally discovered arginylation in the early 1960s and brought it to international recognition. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and thorough, Protein Arginylation: Methods and Protocols would interest the emerging body of scientists involved in the studies of posttranslational arginylation and the rapidly growing community of researchers working on a broad range of posttranslational modifications, the analysis of which often meets similar challenges and utilizes similar principles as posttranslational arginylation."--
Other form:	Print version: Protein Arginylation. Humana Press Inc 2015 9781493929344
Standard no.:	10.1007/978-1-4939-2935-1

Table of Contents:

Protein arginylation: Over 50 years of discovery
Recollection of how we came across the protein modification with amino acids by aminoacyl trna-protein transferase
Arginyltransferase: A personal and historical perspective
Arginylation in a partially purified fraction of 150k x g supernatants of axoplasm and injured vertebrate nerves
Preparation of ate1 enzyme from native mammalian tissues
Correlated measurement of endogenous ate1 activity on native acceptor proteins in tissues and cultured cells to detect cellular aging
Assaying the posttranslational arginylation of proteins in cultured cells
Assaying ate1 activity in yeast by beta-gal degradation
Bacterial expression and purification of recombinant arginyltransferase (ate1) and arg-trna synthetase (rrs) for arginylation assays
Assaying ate1 activity in vitro
High-throughput arginylation assay in microplate format
Assay of arginyltransferase activity by a fluorescent hplc method
Identification of arginylated proteins by mass spectrometry
Analysis of arginylated peptides by subtractive edman degradation
Transferase-mediated labeling of protein n-termini with click chemistry handles
Applying arginylation for bottom-up proteomics
Development of new tools for the studies of protein arginylation.

Protein arginylation : methods and protocols /

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