Immunosenecence : methods and protocols /
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| Imprint: | New York : Humana Press : Springer, ©2015. |
|---|---|
| Description: | 1 online resource (xi, 270 pages) : illustrations (some color) |
| Language: | English |
| Series: | Methods in molecular biology, 1064-3745 ; 1343 Springer protocols Methods in molecular biology (Clifton, N.J.) ; v. 1343. Springer protocols (Series) |
| Subject: | |
| Format: | E-Resource Book |
| URL for this record: | http://pi.lib.uchicago.edu/1001/cat/bib/11250002 |
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| 245 | 0 | 0 | |a Immunosenecence : |b methods and protocols / |c edited by Albert C. Shaw. |
| 260 | |a New York : |b Humana Press : |b Springer, |c ©2015. | ||
| 300 | |a 1 online resource (xi, 270 pages) : |b illustrations (some color) | ||
| 336 | |a text |b txt |2 rdacontent | ||
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| 490 | 1 | |a Methods in molecular biology, |x 1064-3745 ; |v 1343 | |
| 490 | 1 | |a Springer protocols | |
| 504 | |a Includes bibliographical references and index. | ||
| 520 | 8 | |a In this volume, expert researchers detail protocols for evaluating the protean effects of immunosenescence on innate and adaptive immune function, including approaches to the analysis of antigen receptor repertoire. In addition, this book guides readers through cell biology and biochemical methods for analyses of telomere dysfunction, autophagy, protein oxidation, microRNAs, and DNA methylation. | |
| 505 | 0 | |a Isolation of Lipid Rafts from Human Neutrophils by Density Gradient Centrifugation -- Flow Cytometry Analysis of NK Cell Phenotype and Function in Ageing -- Flow Cytometric Identification of Fibrocytes in the Human Circulation -- Experimental Approaches to Tissue Injury and Repair in Advanced Age -- Multicolor Digital Flow Cytometry Used in Human Translational Immunology -- Flow Cytometry-Based Methods to Characterize Immune Senescence in Nonhuman Primates -- Multiparameter Phenotyping of Human PBMCs Using Mass Cytometry -- Imaging Immunosenescence -- Activation-induced Cytidine Deaminase and Switched Memory B Cells as Predictors of Effective In Vivo Responses to the Influenza Vaccine -- Analyzing the Effects of Aging on CD8+ T Cell Phenotype Using Flow Cytometry -- Cell-Mediated Immune Response to Influenza Using Ex Vivo Stimulation and Assays of Cytokine and Granzyme B Responses -- Assays for Monitoring Macroautophagy Activity in T Cells -- Fluorescence-Based Approaches for Quantitative Assessment of Protein Carbonylation, Protein Disulfides and Protein Conformation in Biological Tissues -- Monitoring the DNA Damage Response at Dysfunctional Telomeres -- Single-Cell Analysis of T Cell Receptor ab Repertoire -- Assessment of B Cell Repertoire in Humans -- Laboratory and Data Analysis Methods for Characterization of Human B Cell Repertoires by High-throughput DNA Sequencing -- Discovery of Novel MicroRNAs in Aging C. Elegans -- Analysis of DNA Methylation by Pyrosequencing. | |
| 650 | 0 | |a Immunology |v Laboratory manuals. | |
| 650 | 0 | |a Medicine. |0 http://id.loc.gov/authorities/subjects/sh85083064 | |
| 650 | 0 | |a Immunology. |0 http://id.loc.gov/authorities/subjects/sh85064579 | |
| 650 | 7 | |a Immunology. |2 fast |0 (OCoLC)fst00968006 | |
| 650 | 7 | |a Medicine. |2 fast |0 (OCoLC)fst01014893 | |
| 650 | 7 | |a Medical |x Immunology. |2 bisacsh | |
| 650 | 7 | |a Immunology. |2 bicssc | |
| 650 | 1 | 2 | |a Immune System. |
| 650 | 1 | 2 | |a Immunosenescence. |
| 650 | 2 | 2 | |a Aging. |
| 650 | 0 | 2 | |a Aged. |
| 655 | 4 | |a Electronic books. | |
| 655 | 7 | |a Laboratory manuals. |2 fast |0 (OCoLC)fst01920776 | |
| 700 | 1 | |a Shaw, Albert C. |0 http://id.loc.gov/authorities/names/n2016180162 | |
| 776 | 0 | 8 | |i Print version: |t Immunosenecence |z 9781493929627 |
| 830 | 0 | |a Methods in molecular biology (Clifton, N.J.) ; |v v. 1343. | |
| 830 | 0 | |a Springer protocols (Series) | |
| 880 | 0 | 0 | |6 505-00/(S |t Isolation of lipid rafts from human neutrophils by density gradient centrifugation / |r Carl Fortin and Tamas Fülöp -- |t Flow cytometry analysis of NK cell phenotype and function in aging / |r Raquel Tarazona [and others] -- |t Flow cytometric identification of fibrocytes in the human circulation / |r Xinyuan Hu, Erin M. DeBiasi, and Erica L. Herzog -- |t Experimental approaches to tissue injury and repair in advanced age / |r Aleah L. Brubaker, Stewart R. Carter, and Elizabeth J. Kovacs -- |t Multicolor digital flow cytometry in human translational immunology / |r Samit R. Joshi, Subhasis Mohanty, and Albert C. Shaw -- |t Flow cytometry-based methods to characterize immune senescence in nonhuman primates / |r Christine Meyer [and others] -- |t Multiparameter phenotyping of human PBMCs using mass cytometry / |r Michael D. Leipold, Evan W. Newell, and Holden T. Maecker -- |t Imaging immunosenescence / |r Feng Qian and Ruth R. Montgomery -- |t Activation-induced cytidine deaminase and switched memory B cells as predictors of effective in vivo responses to the influenza vaccine / |r Daniela Frasca, Alain Diaz, and Bonnie B. Blomberg -- |t Analyzing the effect of aging on CD8+ T-cell phenotype using flow cytometry / |r Min Sun Shin and Insoo Kang -- |t Cell-mediated immune response to influenza using ex vivo stimulation and assays of cytokine and granzyme B responses / |r Janet E. McElhaney and Beth Gentleman -- |t Assays for monitoring macroautophagy activity in T cells / |r Yair Botbol and Fernando Macian -- |t Fluorescence-based approaches for quantitative assessment of protein carbonylation, protein disulfides, and protein conformation in biological tissues / |r Asish R. Chaudhuri [and others] -- |t Monitoring the DNA damage response at dysfunctional telomeres / |r Rekha Rai and Sandy Chang -- |t Single-cell analysis of T-cell receptor Αβ repertoire / |r Pradyot Dash, George C. Wang, and Paul G. Thomas -- |t Assessment of B cell repertoire in humans / |r Yu-Chang Wu, David Kipling, and Deborah Dunn-Walters -- |t Laboratory and data analysis methods for characterization of human B cell repertoires by high-throughput DNA sequencing / |r Chen Wang [and others] -- |t Discovery of novel microRNAs in aging Caenorhabditis elegans / |r Alexandre de Lencastre and Frank Slack -- |t Analysis of DNA methylation by pyrosequencing / |r Colin Delaney, Sanjay K. Garg, and Raymond Yung. |
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