Bacterial persistence : methods and protocols /

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Bibliographic Details
Imprint:New York : Humana Press : Springer, ©2016.
Description:1 online resource (xi, 244 pages) : illustrations (some color)
Language:English
Series:Methods in molecular biology, 1064-3745 ; 1333
Springer protocols
Methods in molecular biology (Clifton, N.J.) ; v. 1333.
Springer protocols (Series)
Subject:
Format: E-Resource Book
URL for this record:http://pi.lib.uchicago.edu/1001/cat/bib/11250004
Hidden Bibliographic Details
Other authors / contributors:Michiels, Jan (Professor), editor.
Fauvart, Maarten, editor.
ISBN:9781493928545
1493928546
9781493928538
1493928538
Notes:Includes bibliographical references and index.
Summary:This volume presents a comprehensive collection of methods that have been instrumental to the current understanding of bacterial persisters. Chapters in the book cover topics ranging from general methods for measuring persister levels in Escherichia coli cultures, protocols for the determination of the persister subpopulation in Candida albicans, quantitative measurements of Type I and Type II persisters using ScanLag, to in vitro and in vivo models for the study of the intracellular activity of antibiotics. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Bacterial Persistence: Methods and Protocols brings together the most respected researchers in bacterial persistence whose studies will remain vital to understanding this field for many years to come.
Other form:Print version: Bacterial persistence. New York : Humana Press, [2016] 9781493928538
Table of Contents:
  • Historical perspective on bacterial persistence / Natalie Verstraeten [and others]
  • Persisters : methods for isolation and identifying contributing factors-a review / Sarah E. Rowe [and others]
  • General method for measuring persister levels in Escherichia coli cultures / Niilo Kaldalu [and others]
  • Optimized method for measuring persistence in Escherichia coli with improved reproducibility / F. Goormaghtigh and L. Van Melderen
  • Microplate-based system as in vitro model of biofilm growth and quantification / Ilse Vandecandelaere, Heleen Van Acker, and Tom Coenye
  • Protocol for determination of the persister subpopulation in Candida Albicans biofilms / Katrijn De Brucker [and others]
  • Quantitative measurements of type I and type II persisters using ScanLag / Irit Levin-Reisman and Nathalie Q. Balaban
  • Analyzing persister physiology with fluorescence-activated cell sorting / Mehmet A. Orman [and others]
  • Single-cell detection and collection of persister bacteria in a directly accessible femtoliter droplet array / Ryota Iino [and others]
  • Whole-cell-based high-throughput screening method to identify molecules targeting Pseudomonas Aeruginosa persister cells / Veerle Liebens, Valerie Defraine, and Maarten Fauvart
  • Functional analysis of the role of toxin-antitoxin (TA) loci in bacterial persistence / Aaron T. Butt and Richard W. Titball
  • Experimental evolution of Escherichia coli persister levels using cyclic antibiotic treatments / Bram Van den Bergh, Joran E. Michiels, and Jan Michiels
  • In vitro models for the study of the intracellular activity of antibiotics / Julien M. Buyck [and others]
  • Murine model for Escherichia coli urinary tract infection / Thomas J. Hannan and David A. Hunstad
  • Analysis of macrophage-induced Salmonella persisters / Robert A. Fisher, Angela M. Cheverton, and Sophie Helaine
  • Population dynamics analysis of ciprofloxacin-persistent S. Typhimurium cells in a mouse model for Salmonella diarrhea / Patrick Kaiser, Roland R. Regoes, and Wolf-Dietrich Hardt
  • Computational methods to model persistence / Alexandra Vandervelde [and others].