Hydrocarbon and lipid microbiology protocols : ultrastructure and imaging /

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Bibliographic Details
Imprint:	Berlin : Springer, 2016.
Description:	1 online resource (x, 174 pages)
Language:	English
Series:	Springer protocols handbooks, 1949-2448 Springer protocols (Series)
Subject:	Lipids -- Laboratory manuals. Lipids -- Biotechnology -- Laboratory manuals. Hydrocarbons -- Laboratory manuals. Hydrocarbons. Lipids. Lipids -- Biotechnology. Laboratory manuals.
Format:	E-Resource Book
URL for this record:	http://pi.lib.uchicago.edu/1001/cat/bib/11254845

Hidden Bibliographic Details
Other authors / contributors:	McGenity, Terry J., editor. Timmis, K. N., editor. Nogales, Balbina, editor.
ISBN:	9783662491348 3662491346 9783662491324
Digital file characteristics:	text file PDF
Notes:	Includes bibliographical references. Online resource; title from PDF title page (SpringerLink, viewed April 19, 2016).
Summary:	Annotation This volume presents key microscopy and imaging methods for revealing the structure and ultrastructure of environmental and experimental samples, of microbial communities and cultures, and of individual cells. Method adaptations that specifically address problems concerning the hydrophobic components of samples are highlighted and discussed. The methods described range from electron microscopy and light and fluorescence microscopy, to confocal laser-scanning microscopy, and include experimental set-ups for the analysis of interfacial processes like microbial growth and activities at hydrocarbon:water interfaces, biofilms and microbe:mineral interfaces.
Other form:	Printed edition: 9783662491324
Standard no.:	10.1007/978-3-662-49134-8

Table of Contents:

Introduction
Electron microscopy protocols for the study of hydrocarbon-producing and -decomposing microbes: classical and advanced methods
Protocol for laser scanning microscopy of microorganisms on hydrocarbons
Fluorescence microscopy for microbiology
Imaging bacterial cells and biofilms adhering to hydrophobic organic compounds-water interfaces
Bacteria-mineral colloid interactions in biofilms
an ultrastructural and microanalytical approach
Identification of microorganisms in hydrocarbon contaminated aquifer samples by fluorescence in-situ hybridization (CARD-FISH)
Studies of the ecophysiology of single cells in microbial communities by (quantitative) microautoradiography and fluorescence in situ hybridization (MAR-FISH)
Protocol for in situ detection of functional genes of microorganisms by Two-pass TSA-FISH
Three-dimensional visualization and quantification of lipids in microalgae using confocal laser scanning microscopy
A Correlative Light-Electron Microscopy (CLEM) protocol for the identification of bacteria in animal tissue, exemplified by methanotrophic symbionts of deep-sea mussels.

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