Dendritic cell protocols /

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Bibliographic Details
Edition:	3rd ed.
Imprint:	New York, NY : Humana Press : Springer, ©2016.
Description:	1 online resource (xi, 322 pages) : illustrations (some color)
Language:	English
Series:	Springer protocols Methods in molecular biology, 1940-6029 ; v. 1423 Springer protocols (Series) Methods in molecular biology (Clifton, N.J.) ; v. 1423.
Subject:	Dendritic cells. Medicine. Dendritic Cells. Dendritic cells. Medicine. Laboratory Manual. Laboratory manuals. Laboratory manuals.
Format:	E-Resource Book
URL for this record:	http://pi.lib.uchicago.edu/1001/cat/bib/11255186

Hidden Bibliographic Details
Other authors / contributors:	Segura, Elodie, 1980- editor. Onai, Nobuyuki, editor.
ISBN:	9781493936069 1493936069 1493936042 9781493936045 9781493936045
Notes:	Includes bibliographical references and index.
Summary:	The third edition of this volume is aimed at providing both beginners and more experienced researchers a choice of methods to isolate and analyze dendritic cells(DC). An introductory review provides an overview of recent advances in the characterization of DC subsets in mouse and human. While additional chapters provide methods to culture human and mouse dendritic cells, protocols for the isolation of dendritic cells, the isolation of dendritic cell progenitors from mouse, and the purification of dendritic cells from human blood. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Dendritic Cell Protocols, Third Edition aims to ensure successful results in the further study of this vital field.
Other form:	Print version: Dendritic cell protocols. Humana Press, 2016 9781493936045

Table of Contents:

Review of mouse and human dendritic cell subsets / Elodie Segura
In vitro generation of human XCR1+ dendritic cells from CD34+ hematopoietic progenitors / Sreekumar Balan and Marc Dalod
Derivation and utilization of functional CD8+ dendritic cell lines / Matteo Pigni, Devika Ashok, and Hans Acha-Orbea
Isolation of dendritic cell progenitor and bone marrow progenitor cells from mouse / Nobuyuki Onai and Toshiaki Ohteki
Isolation and enrichment of large numbers of highly purified mouse spleen dendritic cell populations and their in vitro equivalents / David Vremec
Dendritic cell subset purification from human tonsils and lymph nodes / Mélanie Durand and Elodie Segura
Isolation and identification of conventional dendritic cell subsets from the intestine of mice and men / Charlotte L. Scott [and others]
Isolation of human skin dendritic cell subsets / Merry Gunawan, Laura Jardine, and Muzlifah Haniffa
Isolation of mouse dendritic cell subsets and macrophages from the skin / Camille Malosse and Sandrine Henri
Isolation of conventional dendritic cells from mouse lungs / Lianne van de Laar, Martin Guilliams, and Simon Tavernier
Purification of human dendritic cell subsets from peripheral blood / Solana Alculumbre and Lucia Pattarini
Protocols for the identification and isolation of antigen-presenting cells in human and mouse tissues / Naomi McGovern [and others]
Measurement of export to the cytosol in dendritic cells using a cytofluorimetry-based assay / Omar I. Vivar, Joao G. Magalhaes, and Sebastian Amigorena
Cross-presentation assay for human dendritic cells / Elodie Segura
Analysis of intracellular trafficking of dendritic cell receptors for antigen targeting / Haiyin Liu [and others]
Characterization of dendritic cell subsets through gene expression analysis / Thien-Phong Vu Manh and Marc Dalod
In vivo ablation of a dendritic cell subset expressing the chemokine receptor XCR1 / Hiroaki Hemmi, Katsuaki Hoshino, and Tsuneyasu Kaisho
In vivo analysis of intestinal mononuclear phagocytes / Caterina Curato [and others]
In vivo imaging of cutaneous DCs in mice / Gyohei Egawa and Kenji Kabashima
Analysis of dendritic cell function using Clec9A-DTR transgenic mice / Piotr Tetlak and Christiane Ruedl
Analysis of DC functions using CD205-DTR knock-in mice / Tomohiro Fukaya [and others]
Generation of humanized mice for analysis of human dendritic cells / Yasuyuki Saito, Jana M. Ellegast, and Markus G. Manz.

Dendritic cell protocols /

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