Cancer cell culture : methods and protocols /

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Bibliographic Details
Edition:	2nd ed.
Imprint:	Totowa, N.J. : Humana Press, 2011.
Description:	1 online resource (xiv, 502 pages) : illustrations
Language:	English
Series:	Methods in molecular biology, 1064-3745 ; v. 731 Springer protocols Springer protocols (Series) Methods in molecular biology (Clifton, N.J.) ; v. 731.
Subject:	Cancer cells -- Laboratory manuals. Tumor Cells, Cultured. Laboratory Techniques and Procedures Cancer cells. Laboratory Manual. Laboratory manuals.
Format:	E-Resource Book
URL for this record:	http://pi.lib.uchicago.edu/1001/cat/bib/11256839

Hidden Bibliographic Details
Other authors / contributors:	Cree, Ian A.
ISBN:	9781617790805 161779080X 1617790796 9781617790799 9781617790812 1617790818 9781493956579 1493956574
Notes:	Includes bibliographical references and index. English. Print version record.
Summary:	With many recent advances, cancer cell culture research is more important than ever before. This timely edition of Cancer Cell Culture: Methods and Protocols covers the basic concepts of cancer cell biology and culture while expanding upon the recent shift in cell culture methods from the generation of new cell lines to the use of primary cells. There are methods to characterize and authenticate cell lines, to isolate and develop specific types of cancer cells, and to develop new cell line models. Functional assays are provided for the evaluation of clonogenicity, cell proliferation, apoptosis, adhesion, migration, invasion, senescence, angiogenesis, and cell cycle parameters. Other methods permit the modification of cells for transfection, drug resistance, immortalization, and transfer in vivo, the co-culture of different cell types, and the detection and treatment of contamination. In this new edition, specific emphasis is placed on safe working practice for both cells and laboratory researchers. These chapters contain the information critical to success - only by good practice and quality control will the results of cancer cell culture improve. Written in the successful Methods in Molecular Biology"!series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and accessible, Cancer Cell Culture: Methods and Protocols serves as a practical guide for scientists of all backgrounds and aims to convey the appropriate sense of fascination associated with this research field
Other form:	Print version: Cancer cell culture. 2nd ed. Totowa, N.J. : Humana Press, 2011 1617790796
Standard no.:	10.1007/978-1-61779-080-5.

Table of Contents:

Cancer biology
Principles of cancer cell culture
Storage of cell lines
Characterization and authentication of cancer cell lines: An overview
Online verification of human cell line identity by str DNA typing
Cytogenetic analysis of cancer cell lines
Cell culture contamination
Detecting mycoplasma contamination in cell cultures by polymerase chain reaction
Elimination of mycoplasmas from infected cell lines using antibiotics
Quality assurance and good laboratory practice
Generation of lung cancer cell line variants by drug selection or cloning
Isolation and culture of colon cancer cells and cell lines
Isolation and culture of melanoma and naevus cells and cell lines
Isolation and culture of squamous cell carcinoma lines
Isolation and culture of ovarian cancer cells and cell lines
Establishment and culture of leukemia-lymphoma cell lines
Isolation of inflammatory cells from human tumours
Isolation of endothelial cells from human tumors
Cellular chemosensitivity assays: An overview
Cell sensitivity assays: The mtt assay
Cell sensitivity assays: The atp-based tumor chemosensitivity assay
Differential staining cytotoxicity assay: A review
Real-time cytotoxicity assays
Purification of annexin v and its use in the detection of apoptotic cells
Measurement of DNA damage in individual cells using the single cell gel electrophoresis (comet) assay
Molecular breakpoint analysis of chromosome translocations in cancer cell lines by long distance inverse-PCR
Cell migration and invasion assays
Angiogenesis assays
Flow cytometric DNA analysis of human cancers and cell lines
Expression analysis of homeobox genes in leukemia/lymphoma cell lines
Measuring gene expression from cell cultures by quantitative reverse-transcriptase polymerase chain reaction
Proteomic evaluation of cancer cells: Identification of cell surface proteins
Development of rituximab-resistant b-nhl clones: An in vitro model for studying tumor resistance to monoclonal antibody-mediated immunotherapy
Analysis of drug interactions
Transfection and DNA-mediated gene transfer
Drug design and testing: Profiling of antiproliferative agents for cancer therapy using a cell-based methyl-[3H]-thymidine incorporation assay
Feeder layers: Co-culture with nonneoplastic cells
Xenotransplantation of breast cancers.

Cancer cell culture : methods and protocols /

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