Claudins : methods and protocols /

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Bibliographic Details
Imprint:	New York : Humana : Springer, ©2011.
Description:	1 online resource (xiv, 447 pages) : illustrations
Language:	English
Series:	Springer protocols Methods in molecular biology, 1940-6029 ; v. 762 Springer protocols (Series) Methods in molecular biology (Clifton, N.J.) ; v. 762.
Subject:	Tight junctions (Cell biology) Membrane proteins. Proteins. Cell membranes. Cells. Anatomy. Cell junctions. Cell Membrane Structures Proteins Cell Membrane Amino Acids, Peptides, and Proteins Chemicals and Drugs Cellular Structures Cells Anatomy Tight Junctions Claudins Membrane Proteins Intercellular Junctions Animal Structures Proteins Cells Cell membranes Cell junctions Anatomy Membrane proteins Tight junctions (Cell biology) Laboratory Manual Laboratory manuals.
Format:	E-Resource Book
URL for this record:	http://pi.lib.uchicago.edu/1001/cat/bib/11258736

Hidden Bibliographic Details
Other authors / contributors:	Turksen, Kursad.
ISBN:	9781617791857 1617791857 9781617791840 1617791849
Digital file characteristics:	text file
Notes:	Includes bibliographical references and index. English. Print version record.
Summary:	Since the initial discovery of the claudins, the field of tight junctions and cell polarity has become increasingly energized, mainly due to the fact that the claudins have provided an opportunity to understand the molecular basis of the tight junction function and their tissue specific roles. In Claudins: Methods and Protocols, experts and pioneers in the field provide an extensive collection of techniques involving these tight junction proteins. Written in the highly successful Methods in Molecular Biology series format, chapters include brief introductions to their respective topics, detailed lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Claudins: Methods and Protocols serves as an ideal guide for all scientists seeking to further our understanding of this vital area of research.
Other form:	Print version: Claudins. New York : Humana ; Springer, ©2011 1617791849
Standard no.:	10.1007/978-1-61779-185-7

Table of Contents:

Measuring size-dependent permeability of the tight junction using PEG profiling / Christina M. Van Itallie and James M. Anderson
Biochemical analysis of claudin-binding compatibility / Christina Ward and Michael Koval
Electrophysiological Characterization of Claudin Ion Permeability Using Stably Transfected Epithelial Cell Lines / Alan S.L. Yu
Tight junction, intercellular seal as a cell signaling player : protocols for examination of its status / Makoto Osanai
Interactions between Clostridium perfringens enterotoxin and claudins / Susan L. Robertson and Bruce A. McClane
Biophysical methods to probe claudin-mediated adhesion at the cellular and molecular level / Sri Ram Krishna Vedula [and others]
Detection of tight junction barrier function in vivo by biotin / Lei Ding [and others]
Coculture method to examine interactions between claudin isoforms in tight junction-free HEK293 cells and tight junction-bearing MDCK II cells / Tetsuichiro Inai
Claudin-4 : functional studies beyond the tight junction / Holly A. Eckelhoefer [and others]
Methods to analyze subcellular localization and intracellular trafficking of claudin-1 / P. Jaya Kausalya and Walter Hunziker
Claudin family proteins in Caenorhabditis elegans / Jeffrey S. Simske and Jeff Hardin
In vivo imaging of tight junctions using claudin-EGFP transgenic medaka / Tatsuo Miyamoto, Mikio Furuse, and Makoto Furutani-Seiki
Claudins in a primary cultured puffer fish (Tetraodon nigroviridis) gill epithelium / Phuong Bui and Scott P. Kelly
Manipulating claudin expression in avian embryos / Michelle M. Collins and Aimee K. Ryan
Identification of claudins by western blot and immunofluorescence in different cell lines and tissues / Lorenza Gonzalez-Mariscal, Erika Garay, and Miguel Quiros
Expression and function of claudins in hepatocytes / Takashi Kojima and Norimasa Sawada
Analysis of changes in the expression pattern of claudins using salivary acinar cells in primary culture / Junko Fujita-Yoshigaki
Development of biological tools to study claudins in the male reproductive tract / Daniel G. Cyr [and others]
Using molecular tracers to assess the integrity of the intestinal epithelial barrier in vivo / Julian A. Guttman
Laboratory methods in the study of endometrial claudin-4 / Paulo Serafini [and others]
Role of claudins in oxidant-induced alveolar epithelial barrier dysfunction / Yu Sun, Richard D. Minshall, and Guochang Hu
Tracing the endocytosis of claudin-5 in brain endothelial cells / Svetlana M. Stamatovic, Richard F. Keep, and Anuska V. Andjelkovic
Quantitative in situ analysis of claudin expression at the blood-retinal barrier / Heping Xu and Janet Liversidge
MMP-mediated disruption of claudin-5 in the blood-brain barrier of rat brain after cerebral ischemia / Yi Yang and Gary A. Rosenberg
Claudin-5 expression in in vitro models of the blood-brain barrier / Itzik Cooper, Katayun Cohen-Kashi-Malina, and Vivian I. Teichberg
HIV-1-induced alterations of claudin-5 expression at the blood-brain barrier level / Ibolya E. Andras and Michal Toborek
Enhanced immunohistochemical resolution of claudin proteins in glycolmethacrylate-embedded tissue biopsies / Jane E. Collins [and others]
Claudin-16/paracellin-1, cloning, expression, and its role in tight junction functions in cancer and endothelial Cells / Tracey A. Martin and Wen G. Jiang
Dynamics of claudins expression in colitis and colitis-associated cancer in rat / Yoshiaki Arimura, Kanna Nagaishi, and Masayo Hosokawa
Anti-claudin-4-conjugated highly luminescent nanoparticles as biological labels for pancreatic cancer sensing / Ken-Tye Yong.

Claudins : methods and protocols /

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