Regulatory T cells : methods and protocols /

Regulatory T cells : methods and protocols /

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Bibliographic Details
Imprint:New York : Humana Press, ©2011.
Description:1 online resource (xi, 282 pages)
Language:English
Series:Methods in molecular biology, 1064-3745 ; v. 707
Methods in molecular biology (Clifton, N.J.) ; v. 707.
Subject:
Immune response -- Regulation.
T cells.
T-Lymphocytes, Regulatory.
Immune response -- Regulation.
T cells.
Format: E-Resource Book
URL for this record:http://pi.lib.uchicago.edu/1001/cat/bib/11259684
Hidden Bibliographic Details
Other authors / contributors:Kassiotis, George.
Liston, Adrian.
ISBN:9781617379796
1617379794
9781617379789
1617379786
Digital file characteristics:text file PDF
Notes:Includes index.
Includes bibliographical references and index.
English.
Print version record.
Summary:Driven by methodological success in identifying reliable lineage markers, regulatory T cells have quickly been recognized as the most numerous subset of immune regulators in the body with critical functions in a wide array of immune responses. In Regulatory T Cells: Methods and Protocols, experts in the field to offer a collection of current techniques to advance the study of regulatory T cells, including the use of the IL-2 receptor alpha chain and other markers, as well as the more recently desirable use of the transcription factor FoxP3. Divided into three sections, the book covers, in equal measure, in vitro, in vivo, and human studies. Written in the highly successful Methods in Molecular Biology series format, chapters include brief introductions to their respective subjects, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known methodological pitfalls. Authoritative and cutting-edge, Regulatory T Cells: Methods and Protocols distills the most vital current techniques through several years of optimization and standardization in order to allow reliable and reproducible use by both young and experienced cellular and molecular immunologists.
Other form:Print version: Regulatory T cells. New York : Humana Press, ©2011
Standard no.:10.1007/978-1-61737-979-6
Table of Contents:
  • Regulatory T cells: history and perspective / S. Sakaguchi
  • In vitro Treg suppression assays / L.W. Collison and D.A. Vignali
  • Generation of T cell hybridomas from naturally occurring FoxP3(+) regulatory T cells / N. Singh [and others]
  • In vitro and in vivo analyses of regulatory T cell suppression of CD8(+) T cells / K.J. Hasenkrug and L.M. Myers
  • Flow cytometric profiling of mature and developing regulatory T cells in the thymus / D.M. Simons and A.J. Caton
  • Chip-on-chip for FoxP3 / Y. Zheng
  • Live imaging of dendritic cell-Treg cell interactions / M. Sarris and A.G. Betz
  • Genetic tools for analysis of FoxP3(+) Regulatory T cells in vivo / N.M. Jeremiah and A. Liston
  • In vivo Treg suppression assays / C.J. Workman [and others]
  • In vivo depletion of FoxP3(+) Tregs using the dereg mouse model / K. Lahl and T. Sparwasser
  • Antigen-specific induction of regulatory T cells in vivo and in vitro / C. Daniel, H. Ploegh and H. von Boehmer
  • In vitro expansion of alloantigen-specific regulatory T cells and their use in prevention of allograft rejection / C. Nouze, L. Pasquet and J.P. van Meerwijk
  • Analysis of human FoxP3(+) Treg cells phenotype and function / E. d'Hennezel and C.A. Piccirillo
  • Depletion of human regulatory T cells / A.C. Hobeika [and others]
  • Assessment of suppressive capacity by human regulatory T cells using a reproducible, bi-directional CFSE-based in vitro assay / A. Schneider and J.H. Buckner
  • Measurement of proliferation and disappearance of regulatory T cells in human studies using deuterium-labeled glucose / M. Vukmanovic-Stejic [and others]
  • Flow cytometric detection of human regulatory T cells / B.F. de St Groth [and others].

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