Type-1 diabetes : methods and protocols /

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Bibliographic Details
Imprint:	New York, NY : Humana Press, 2016.
Description:	1 online resource (x, 213 pages) : illustrations (some color)
Language:	English
Series:	Methods in molecular biology, 1064-3745 ; 1433 Methods in molecular biology (Clifton, N.J.) ; v. 1433.
Subject:	Diabetes. Diabetes Mellitus, Type 1. Diabetes.
Format:	E-Resource Book
URL for this record:	http://pi.lib.uchicago.edu/1001/cat/bib/11263555

Hidden Bibliographic Details
Varying Form of Title:	Type one diabetes
Other authors / contributors:	Gillespie, Kathleen M., editor.
ISBN:	9781493936434 1493936433 1493936417 9781493936410 9781493936410
Notes:	Includes index. Includes bibliographical references and index. Online resource; title from PDF title page (SpringerLink, viewed June 10, 2016).
Summary:	The aim of this volume is to present current methodologies to predict and understand the pathogenesis of Type-1 Diabetes for clinical and non-clinical researchers. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Practical and reliable, Type-1 Diabetes: Methods and Protocols will aid researchers in using these methods to advance their own studies.
Other form:	Print version: Type-1 diabetes. New York, NY : Humana Press, 2016
Standard no.:	10.1007/978-1-4939-3643-4

Table of Contents:

Type 1 diabetes: Current perspectives
Type 1 diabetes high-risk HLA class ii determination by polymerase chain reaction sequence-specific primers
Pancreatic beta cell survival and signaling pathways: Effects of type 1 diabetes-associated genetic variants
Islet autoantibody analysis: Radioimmunoassays
Islet autoantibody detection by electrochemiluminescence (ECL) assay
Detection of C-peptide in urine as a measure of ongoing beta cell function
Histology of type 1 diabetes pancreas
Identification of islet antigen-specific CD8 T cells using mhci-peptide tetramer reagents in the non obese diabetic (NOD) mouse model of type 1 diabetes
Tracking immunological responses of islet antigen-specific T cells in the nonobese diabetic (NOD) mouse model of type 1 diabetes
Adoptive transfer of autoimmune diabetes using immunodeficient nonobese diabetic (NOD) mice
Methylation analysis in distinct immune cell subsets in type 1 diabetes
Fluorescence in situ hybridization with concomitant immunofluorescence in human pancreas
Laser capture and single cell genotyping from frozen tissue sections
The gut microbiome in the NOD mouse
Molecular methods and protein synthesis for definition of autoantibody epitopes.

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