Genome editing /

Genome editing /

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Bibliographic Details
Imprint:Switzerland : Springer, 2016.
Description:1 online resource (ix, 166 pages) : color illustrations
Language:English
Subject:
Genetic engineering.
Stem cells.
Genetic Engineering.
Genetic engineering.
Stem cells.
Electronic books.
Format: E-Resource Book
URL for this record:http://pi.lib.uchicago.edu/1001/cat/bib/11265246
Hidden Bibliographic Details
Other authors / contributors:Turksen, Kursad, editor.
ISBN:9783319341484
3319341480
9783319341460
3319341464
Notes:Includes index.
Online resource; title from PDF title page (SpringerLink, viewed August 24, 2016).
Summary:This timely volume explores the use of CRISPR-Cas9 for genome editing, presenting cutting-edge techniques and their applications in treatment of disease. The chapters describe latest methods such as use of targetable nucleases, investigation of the non-coding genome, mouse genome editing, increasing of knock-in efficiency in mouse zygotes, and generation of reporter stem cells; the text contextualizes these methods in treatment of cardiovascular disease, diabetes mellitus, retinitis pigmentosa, and others. The final chapters round out the book with a discussion of controversies and future directions. Genome Editing is an essential, of-the-moment contribution to this rapidly growing field. Drawing from a wealth of international perspectives, it presents novel techniques and applications for the engineering of the human genome. This book is essential reading for all clinicians and researchers in stem cells, regenerative medicine, genomics, biochemical and biomedical engineering- especially those interested in learning more about genome editing and applying it in a targeted, specific way.
Other form:Print version: Genome editing. Switzerland : Springer, 2016 3319341464 9783319341460
Table of Contents:
  • Contributors; Genome Editing with Targetable Nucleases; Introduction; Zinc-Finger Nucleases; Transcription Activator-Like Effector Endonucleases; RNA-Guided Nucleases; Target Specificity; Genome Editing with Targetable Nucleases; Promoting Homologous Recombination over Nonhomologous End-Joining; Delivery Methods; Applications; Conclusion; References; CRISPR/Cas9 Approaches to Investigate the Noncoding Genome; Introduction; Genetic Manipulations of Noncoding Sequences in the Prenuclease Era; Editing by Chromosomal Deletion; Epigenome Editing; Targeting Noncoding RNAs
  • ConclusionReferences; At the Conflux of Human Genome Engineering and Induced Pluripotency; Induced Pluripotency and the Human Genetic Model Organism In Vitro; Rise of the Genome Editing Machines; Adding Function to iPSCs Through Transgenesis at Safe-­Harbor Loci; Achieving Seamless Genome Engineering for Accurate Disease Models; Avoiding Unwanted Outcomes: Off-Target Cleavage and Mosaicism; Selection of Isogenic Clones and Technical Controls; Conclusions; References; CRISPR/Cas9 and the Paradigm Shift in Mouse Genome Manipulation Technologies; Introduction
  • Traditional Mouse Genome Engineering Technologies in the Pre-CRISPR Era Traditional Tg Techniques and Their Limitations; Traditional KO/KI Techniques and Their Limitations; Microinjection and Its Limitations; CRISPR/Cas9 and Mouse Genome Editing; CRISPR Technology and the Paradigm Shifts in Mouse Genome Engineering; The Current Challenges of CRISPR/Cas9-Mediated Mouse Genome Engineering; Poor Efficiency of Insertion of Sequences at Cas9 Cut Sites; Challenges in Developing Conditional KO Models; Off-Target Effects; Challenges Associated with Genotyping
  • Future Impact of CRISPR/Cas9 on Manipulating the Mouse Genome Impact on Random Tg Technologies; Impact on KO/KI Technologies; Impact on Microinjection Technique; Conclusion; References; Genome-Editing Technology in CRISPR/Cas System: How to Increase Knock-In Efficiency in Mouse Zygotes; Introduction; CRISPR/Cas System; Delivery System of Cas9 and gRNA; Single-Stranded Oligo-DNA and Double-Stranded DNA as Donor Templates; Molecules That Increase HDR Efficiency; Cell Cycle; Perspective; Conclusion; References; Developments in the Generation of Reporter Stem Cells; Introduction
  • Gene Reporter Pluripotent Lines as Cell Models Methods for the Generation of Reporter Pluripotent Stem Cells; Minimal Promoter-Driven Reporter Expression; Nonintegrational Methods; Integrational Methods; Reporter Knock-In into Endogenous Promoter Site; Homologous Recombination; Mega Nucleases; Zinc-Finger Nucleases; TALEN; CRISPR; Alternate Approaches; Potential Uses of Reporter Stem Cells; References; Current Status of Genome Editing in Cardiovascular Medicine; Cardiovascular Disease Genomics; Precision Medicine; The Genetics of Cardiovascular Disease

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