Methods for analysis of carbohydrate metabolism in photosynthetic organisms : plants, green algae and cyanobacteria /

Methods for analysis of carbohydrate metabolism in photosynthetic organisms : plants, green algae and cyanobacteria /

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Bibliographic Details
Author / Creator:Pontis, Horacio G.
Imprint:London : Academic Press, 2016, ©2017.
Description:1 online resource (252 pages)
Language:English
Subject:
Carbohydrates -- Metabolism.
Photosynthates.
Carbohydrates -- Metabolism.
Photosynthates.
Format: E-Resource Book
URL for this record:http://pi.lib.uchicago.edu/1001/cat/bib/11266555
Hidden Bibliographic Details
ISBN:0128034033
9780128034033
9780128033968
0128033967
Digital file characteristics:text file
Notes:Includes index.
Print version record.
Summary:Methods for Analysis of Carbohydrate Metabolism in Photosynthetic Organisms: Plants, Green Algae and Cyanobacteria examines both general and detailed aspects of carbohydrate metabolism in photosynthetic organisms, along with the four main oligosaccharides and each enzymatic reaction that gives birth to them. Chapters include information on how biological active protein is extracted for different cells, determination of enzymatic activity, separation of proteins by different available methods, and descriptions of analytical methods for the determination of various types of carbohydrates in photosynthetic organisms. The book contains useful protocols for researchers working on the determination of carbohydrate metabolism. The book provides foundational content as well as step-by-step guidance on how to design and conduct an experiment, including what other methodologies could be used if advanced instruments are not readily available. Includes a variety of analytical methods and how to apply the methods using examples from specific case studies Discusses technical information on how to characterize plant carbohydrates and sugar nucleosides Contains easy-to-follow protocols with detailed explanations for self-guidance Provides foundational content as well as step-by-step guidance on how to design and conduct an experiment.
Other form:Print version: Pontis, Horacio G. Methods for analysis of carbohydrate metabolism in photosynthetic organisms. [Place of publication not identified] : Elsevier Academic Press, 2016 0128033967 9780128033968
Table of Contents:
  • Front Cover; Methods for Analysis of Carbohydrate Metabolism in Photosynthetic Organisms; Copyright Page; Dedication; Contents; Biography; Preface; I: General Analytical Methods; 1 Determination of Carbohydrates Metabolism Molecules; 1.1 Introduction; 1.2 Determination of Reducing Sugars by the Somogyi-Nelson Method; 1.3 Determination of Total Sugars by the Anthrone Method; 1.4 Determination of Total Sugars by the Phenol-Sulfuric Acid Assay; 1.5 Enzymatic Determination of Glucose and Fructose; 1.6 Enzymatic Determination of Sucrose.
  • 1.6.1 Spectrophotometric Method (Measurement of NADPH Absorption)1.6.2 Direct Fluorometric Method (Measurement by NADPH Native Fluorescence); 1.6.3 Assay With Fluorescence Enhancement; 1.7 Determination of Glucose and Galactose by an Enzymatic Colorimetric Method; 1.8 Determination of Glucose by an Enzymatic Fluorometric Method; 1.9 Determination of Amino Sugars by the Elson-Morgan Method; 1.10 Determination of Acetyl Amino Sugars by the Morgan-Elson Method; 1.11 Determination of Uronic Acids by the Carbazole Assay; 1.12 Determination of Pentoses by the Orcinol Procedure.
  • 1.13 Determination of Fructose Derivatives by the Thiobarbituric Acid Assay1.14 Determination of Inorganic Phosphate by the Fiske-Subbarow Assay; 1.15 Determination of Inorganic Phosphate by the Chifflet's Method; 1.16 Determination of Uridine Diphosphate by the Measurement of Pyruvate; 1.16.1 Colorimetric Method Using 2,4-Dinitrophenylhydrazine; 1.16.2 Spectrophotometric Method Using Lactic Dehydrogenase; 1.17 Determination of UDP-Glucose Using UDP-Glucose Dehydrogenase; 1.18 Determination of UDP-Glucose Using UDP-Glucose Pyrophosphorylase; Further Reading and References.
  • II: Purification and Analysis of Proteins and Carbohydrates2 Preparation of Protein Extracts; 2.1 Introduction; 2.2 Extraction of Proteins; 2.2.1 Source Material; 2.2.2 Homogenization Buffer Composition; 2.2.3 Breakdown of Biological Material (Cells and Tissues Disruption); 2.2.4 Cell Permeabilization; 2.3 Laboratory Procedures for Protein Extraction; 2.3.1 Preparation of Acetone Powder from Wheat Leaves (Calderón and Pontis, 1985); 2.3.2 Preparation of Plant Tissue Extracts; 2.3.2.1 Preparation of leaf or seedling extracts.
  • 2.3.2.2 Preparation of protein extracts from spinach and Arabidopsis chloroplasts2.3.3 Preparation of Extracts from Photosynthetic Microorganisms; 2.3.3.1 Chlorella vulgaris; 2.3.3.2 Chlamydomonas reinhardtii; 2.3.3.3 Ostreococcus tauri; 2.3.3.4 Cyanobacteria; 2.4 Protein Extract Desalting; Further Reading and References; 3 Protein and Carbohydrate Separation and Purification; 3.1 Introduction; 3.2 Enzymatic Protein Purification; 3.2.1 Methods Based on Protein Solubility; 3.2.2 Protein Fractionation by Batch Adsorption; 3.2.3 Protein Fractionation by Chromatographic Techniques.

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