Fluorescence in situ hybridization (FISH) : application guide /

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Bibliographic Details
Edition:Second edition.
Imprint:[New York, NY] : Springer, 2017.
Description:1 online resource (xiii, 606 pages) : illustrations (some color).
Language:English
Series:Springer Protocols Handbooks
Springer protocols
Springer protocols (Series)
Subject:
Format: E-Resource Book
URL for this record:http://pi.lib.uchicago.edu/1001/cat/bib/11268085
Hidden Bibliographic Details
Other authors / contributors:Liehr, Thomas, 1965-
ISBN:9783662529591
3662529599
9783662529577
3662529572
Notes:Title from PDF title page (SpringerProtocols, viewed October 24, 2016).
Includes bibliographical references and index.
Summary:This manual offers detailed protocols for fluorescence in situ hybridization (FISH) and comparative genomic hybridization approaches, which have been successfully used to study various aspects of genomic behavior and alterations. Methods using different probe and cell types, tissues and organisms, such as mammalians, fish, amphibians (including lampbrush-chromosomes), insects, plants and microorganisms are described in 57 chapters. In addition to multicolor FISH procedures and special applications such as the characterization of marker chromosomes, breakpoints, cryptic aberrations, nuclear architectures and epigenetic changes, as well as comparative genomic hybridization studies, this 2nd edition describes how FISH can be combined with other techniques. The latter include immunostaining, electron microscopy, single cell electrophoresis and microdissection. This well-received application guide provides essential protocols for beginning FISHers and FISH experts alike working in the fields of human genetics, microbiology, animal and plant sciences.
Other form:Print version: Fluorescence in situ hybridization (FISH) : application guide. New York, NY : Springer, ©2017 9783662529577
Table of Contents:
  • Background / Thomas Liehr and Anja Weise
  • Microscopy and imaging / Ivan Y. Iourov
  • Optical filters and light sources for FISH / Michael Sommerauer, Ingrid Feuerbacher, and Alexander Krause
  • Classification of FISH probes / Thomas Liehr Commercial FISH Probes / Thomas Liehr
  • Generation of Paint Probes from Flow-Sorted and Microdissected Chromosomes / Fengtang Yang [and others]
  • FISH-microdissection / Nadezda Kosyakova, Thomas Liehr, and Ahmed B. Hamid Al-Rikabi
  • Homemade locus-specific FISH probes : bacterial artificial chromosomes / Thomas Liehr
  • Standard FISH procedure / Thomas Liehr [and others]
  • Microwave treatment for better FISH results in a shorter time / Anja Weise and Thomas Liehr-- FISH with and without COT1 DNA / Vladimir A. Trifonov [and others]
  • Formamide-free fluorescence in situ hybridization (FISH) / Emanuela V. Volpi
  • One-Day Quick FISH / Gabor Mehes, Tamas Csonka, and Katalin Hegyi
  • Telomere length measurement by FISH / Gordana Joksic [and others]
  • RNA imaging in living cells / Bin Ma and Naoko Tanese
  • Replicative detargeting FISH (ReD-FISH) technique in studies of telomere replication / Nikolay Rubtsov and Natalya Zhdanova
  • Pre- and postnatal diagnostics and research on peripheral blood, bone marrow, chorion, amniocytes, and fibroblasts / Anja Weise and Thomas Liehr
  • Application of FISH to previously GTG-banded and/or embedded cytogenetic slides / Thomas Liehr and Monika Ziegler
  • FISH in uncultivated amniocytes / Anja Weise, Monika Ziegler, and Thomas Liehr
  • Tumorcytogenetic diagnostics and research on blood and bone marrow smears or effusions / Eyad Alhourani [and others]
  • Characterization of mosaicism in different easy-to-acquire body tissues such as buccal smears, skin abrasions, hair root cells, or urine / Thomas Liehr and Nadezda Kosyakova
  • Characterization of archived formalin-fixed/paraffin-embedded or cryofixed tissue, including nucleus extraction / Thomas Liehr
  • FISH on sperm, spermatocytes and oocytes / Maria Oliver-Bonet
  • Two- to three-color FISH / Thomas Liehr, Sven Hauke, and Britta Meyer
  • Multiplex FISH and spectral karyotyping / Thomas Liehr and Nadezda Kosyakova
  • FISH banding techniques / Thomas Liehr, Nadezda Kosyakova, and Anja Weise
  • cenM-FISH approaches / Thomas Liehr, Anja Weise, and Nadezda Kosyakova
  • Heterochromatin-directed mFISH (HCM-FISH) / Thomas Liehr [and others]
  • Subtelomeric and/or subcentromeric probe sets / Anja Weise and Thomas Liehr
  • Bar coding is back / Thomas Liehr, Ahmed B. Hamid Al-Rikabi, and Anja Weise
  • Fluorescence in situ hybridization onto DNA fibres generated using molecular combing / Sandra Louzada, Jun Komatsu, and Fengtang Yang
  • Parental origin determination FISH : pod-FISH / Anja Weise and Thomas Liehr
  • Simultaneous fluorescence Immunostaining and FISH / Christine J. Ye, Guo Liu, and Henry H.Q. Heng
  • RNA-directed FISH and immunostaining / Bin Ma and Naoko Tanese
  • Immunofluorescence staining for cytosine modifications like 5-Methylcytosine and its oxidative derivatives and FISH / Anna A. Pendina [and others]
  • CENP antibodies used additionally to FISH / Elisabeth Klein and Thomas Liehr
  • Interphase FISH in diagnostics / Thomas Liehr and Sven Hauke
  • Interphase FISH for detection of chromosomal mosaicism / Ivan Y. Iourov, Svetlana G. Vorsanova, and Yuri B. Yurov
  • Comet-FISH / Galina Hovhannisyan and Rouben Aroutiounian
  • Micronucleus FISH / Galina Hovhannisyan, Tigran Harutyunyan, and Thomas Liehr
  • Three-dimensional interphase analysis enabled by suspension FISH / Thomas Liehr and Nadezda Kosyakova
  • Animal probes and ZOO-FISH / Fengtang Yang and Alexander S. Graphodatsky
  • Three-dimensional immunofluorescence in situ hybridization in preimplantation mouse embryos / Tiphaine Aguirre-Lavin and Nathalie Beaujean
  • Fish-FISH : molecular cytogenetics in Fish species / Cassia Fernanda Yano, Luiz Antonio Carlos Bertollo, and Marcelo de Bello Cioffi
  • FISH in lampbrush chromosomes / Anna Zlotina and Alla Krasikova
  • General protocol of FISH for insects / Ana Paula Alves-Silva, Luısa Antonia Campos Barros, and Silvia das Gracas Pompolo
  • FISH in Drosophila / Amanda M. Larracuente
  • FISH on insect cells transfected with heterologous DNA / Thomas Liehr
  • In situ hybridization to plant chromosomes / Ekaterina D. Badaeva [and others]
  • Yeast chromosome dynamics revealed by immuno FISH / Harry Scherthan
  • FISHing for food microorganisms / Benedetta Bottari [and others]
  • Evaluation of polymicrobial involvement using fluorescence in situ hybridization (FISH) in clinical practice / Alexander Swidsinski and Vera Loening-Baucke
  • Gold-FISH : in situ hybridization of microbial cells for combined fluorescence and scanning electron microscopy / Hannes Schmidt and Thilo Eickhorst
  • Comparative genomic hybridization (CGH) and microdissection-based CGH (Micro-CGH) / Thomas Liehr, Anita Glaser, and Nadezda Kosyakova
  • Array CGH / Eftychia Dimitriadou and Joris R. Vermeesch
  • Breakpoint mapping of balanced chromosomal rearrangements using array CGH of microdissection-derived FISH probes / Maria Isabel Melaragno and Mariana Moyses-Oliveira
  • Sequencing of microdissection-derived FISH probes / Jirı Stika and Oldrich Mazal.