Heterologous gene expression in E. coli : methods and protocols /
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| Imprint: | New York, NY : Humana Press : Springer, [2017] ©2017 |
|---|---|
| Description: | 1 online resource (xiv, 429 pages) : illustrations (some color) |
| Language: | English |
| Series: | Methods in molecular biology, 1064-3745 ; 1586 Methods in molecular biology (Clifton, N.J.) ; v. 1586. |
| Subject: | |
| Format: | E-Resource Book |
| URL for this record: | http://pi.lib.uchicago.edu/1001/cat/bib/11273689 |
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| 245 | 0 | 0 | |a Heterologous gene expression in E. coli : |b methods and protocols / |c edited by Nicola A. Burgess-Brown. |
| 264 | 1 | |a New York, NY : |b Humana Press : |b Springer, |c [2017] | |
| 264 | 4 | |c ©2017 | |
| 300 | |a 1 online resource (xiv, 429 pages) : |b illustrations (some color) | ||
| 336 | |a text |b txt |2 rdacontent | ||
| 337 | |a computer |b c |2 rdamedia | ||
| 338 | |a online resource |b cr |2 rdacarrier | ||
| 347 | |a text file |b PDF |2 rda | ||
| 490 | 1 | |a Methods in molecular biology, |x 1064-3745 ; |v 1586 | |
| 504 | |a Includes bibliographical references and index. | ||
| 505 | 0 | 0 | |t Recombinant protein expression in E. coli : a historical perspective / |r Opher Gileadi -- |t N- and C-terminal truncations to enhance protein solubility and crystallization : predicting protein domain boundaries with bioinformatics tools / |r Christopher D.O. Cooper and Brian D. Marsden -- |t Harnessing the profinity eXact system for expression and purification of heterologous proteins in E. coli / |r Yoav Peleg, Vadivel Prabahar, Dominika Bednarczyk, and Tamar Unger -- |t ESPRIT : a method for defining soluble expression constructs in poorly Understood Gene Sequences / |r Philippe J. Mas and Darren J. Hart -- |t Optimizing expression and solubility of proteins in E. coli using modified media and induction parameters / |r Troy Taylor, John-Paul Denson, and Dominic Esposito -- |t Optimization of membrane protein production using titratable strains of E. coli / |r Rosa Morra, Kate Young, David Casas-Mao, Neil Dixon, and Louise E. Bird -- |t Optimizing E. coli-based membrane protein production using Lemo21(DE3) or pReX and GFP-fusions / |r Grietje Kuipers, Markus Peschke, Nurzian Bernsel Ismail, Anna Hjelm, Susan Schlegel, David Vikström, Joen Luirink, and Jan-Willem de Gier -- |t High yield of recombinant protein in shaken E. coli cultures with enzymatic glucose release medium EnPresso B / |r Kaisa Ukkonen, Antje Neubauer, Vinit J. Pereira, and Antti Vasala -- |t Generic protocol for purifying disulfide-bonded domains and random protein fragments using fusion proteins with SUMO3 and cleavage by SenP2 protease / |r Hüseyin Besir -- |t Strategy for production of correctly folded disulfide-rich peptides in the periplasm of E. coli / |r Natalie J. Saez, Ben Cristofori-Armstrong, Raveendra Anangi, and Glenn F. King -- |t Split GFP complementation as reporter of membrane protein expression and stability in E. coli : a tool to engineer stability in a LAT transporter / |r Ekaitz Errasti-Murugarren, Arturo Rodríguez-Banqueri, and José Luis Vázquez-Ibar -- |t Acting on folding effectors to improve recombinant protein yields and functional quality / |r Ario de Marco -- |t Protein folding using a vortex fluidic device / |r Joshua Britton, Joshua N. Smith, Colin L. Raston, and Gregory A. Weiss -- |t Removal of affinity tags with TEV protease / |r Sreejith Raran-Kurussi, Scott Cherry, Di Zhang, and David S. Waugh -- |t Generation of recombinant n-linked glycoproteins in E. coli / |r Benjamin Strutton, Stephen R.P. Jaffé, Jagroop Pandhal, and Phillip C. Wright -- |t Production of protein kinases in E. coli / |r Charlotte A. Dodson -- |t Expression of prokaryotic integral membrane proteins in E. coli / |r James D. Love -- |t Multiprotein complex production in E. coli : the SecYEG-SecDFYajC-YidC holotranslocon / |r Imre Berger, Quiyang Jiang, Ryan J. Schulze, Ian Collinson, and Christiane Schaffitzel -- |t Membrane protein production in E. coli lysates in presence of preassembled nanodiscs / |r Ralf-Bernhardt Rues, Alexander Gräwe, Erik Henrich, and Frank Bernhard -- |t Not limited to E. coli : versatile expression vectors for mammalian protein expression / |r Katharina Karste, Maren Bleckmann, and Joop van den Heuvel -- |t Generic protocol for intracellular expression of recombinant proteins in Bacillus subtilis / |r Trang Phan, Phuong Huynh, Tuom Truong, and Hoang Nguyen -- |t In vivo biotinylation of antigens in E. coli / |r Susanne Gräslund, Pavel Savitsky, and Susanne Müller-Knapp -- |t Cold-shock expression system in E. coli for protein NMR studies / |r Toshihiko Sugiki, Toshimichi Fujiwara, and Chojiro Kojima -- |t High-throughput production of proteins in E. coli for structural studies / |r Charikleia Black, John J. Barker, Richard B. Hitchman, Hok Sau Kwong, Sam Festenstein, and Thomas B. Acton -- |t Mass spectrometric analysis of proteins / |r Rod Chalk -- |t How to determine interdependencies of glucose and lactose uptake rates for heterologous protein production with E. coli / |r David J. Wurm, Christoph Herwig, and Oliver Spadiut -- |t Interfacing biocompatible reactions with engineered Escherichia coli / |r Stephen Wallace and Emily P. Balskus. |
| 588 | 0 | |a Online resource; title from PDF title page (springerProtocols, viewed May 4, 2017). | |
| 520 | |a This detailed volume provides a toolbox for designing constructs, tackling expression and solubility issues, handling membrane proteins and protein complexes, and exploring innovative engineering of E. coli. The topics are largely grouped under four parts: high-throughput cloning, expression screening, and optimization of expression conditions, protein production and solubility enhancement, case studies to produce challenging proteins and specific protein families, as well as applications of E. coli expression. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Heterologous Gene Expression in E. coli: Methods and Protocols serves molecular biologists, biochemists and structural biologists, those in the beginning of their research careers to those in their prime, to give both an historical and modern overview of the methods available to express their genes of interest in this exceptional organism. | ||
| 650 | 0 | |a Escherichia coli |x Genetics |v Laboratory manuals. | |
| 650 | 0 | |a Gene expression |v Laboratory manuals. | |
| 650 | 2 | |a Escherichia coli |x genetics. | |
| 650 | 2 | |a Gene Expression. | |
| 650 | 7 | |a Microbiology (non-medical). |2 bicssc | |
| 650 | 7 | |a Science |x Life Sciences |x Biology |x Microbiology. |2 bisacsh | |
| 650 | 7 | |a Escherichia coli |x Genetics. |2 fast |0 (OCoLC)fst00915164 | |
| 650 | 7 | |a Gene expression. |2 fast |0 (OCoLC)fst00939613 | |
| 655 | 2 | |a Laboratory Manual. | |
| 655 | 4 | |a Electronic books. | |
| 655 | 7 | |a Laboratory manuals. |2 fast |0 (OCoLC)fst01920776 | |
| 700 | 1 | |a Burgess-Brown, Nicola A., |e editor. | |
| 776 | 0 | 8 | |i Print version: |t Heterologous gene expression in E. coli. |d New York, NY : Humana Press, ©2017 |z 9781493968855 |
| 830 | 0 | |a Methods in molecular biology (Clifton, N.J.) ; |v v. 1586. | |
| 903 | |a HeVa | ||
| 929 | |a oclccm | ||
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| 928 | |t Library of Congress classification |a QR82.E6 |l Online |c UC-FullText |u https://link.springer.com/10.1007/978-1-4939-6887-9 |z Springer Nature |g ebooks |i 12545479 | ||