Heterologous protein production in CHO cells : methods and protocols /

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Bibliographic Details
Imprint:	New York, NY : Humana Press, 2017.
Description:	1 online resource (x, 252 pages) : illustrations (some color)
Language:	English
Series:	Methods in molecular biology, 1064-3745 ; volume 1603 Methods in molecular biology (Clifton, N.J.) ; v. 1603.
Subject:	Recombinant proteins -- Laboratory manuals. Cell culture -- Laboratory manuals. Pharmaceutical biotechnology -- Laboratory manuals. CHO Cells. Epithelial Cells. Recombinant Proteins -- biosynthesis. Cell culture. Pharmaceutical biotechnology. Recombinant proteins. Laboratory Manual. Laboratory manuals.
Format:	E-Resource Book
URL for this record:	http://pi.lib.uchicago.edu/1001/cat/bib/11273983

Hidden Bibliographic Details
Other authors / contributors:	Meleady, Paula, editor.
ISBN:	9781493969722 1493969722 1493969714 9781493969715 9781493969715
Digital file characteristics:	text file PDF
Notes:	Includes bibliographical references and index. Online resource; title from PDF title page (SpringerLink, viewed May 18, 2017).
Summary:	This volume discusses protocols that cover genetic manipulation of Chinese hamster ovary (CHO) cells for recombinant protein production, and protocols for the characterization of CHO cells using 'omic approaches. This book also explores methods that discuss the genome editing tool, CRISPR/Cas9, and the characterization of recombinant protein products, such as glycosylation and host cell protein analysis. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Comprehensive and cutting-edge, Heterologous Protein Production in CHO Cells: Methods and Protocols is a valuable resource for scientists and researchers who are interested in further studying cell production in CHO cells.
Other form:	Printed edition: 9781493969715
Standard no.:	10.1007/978-1-4939-6972-2 9781493969715

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