Directed evolution library creation : methods and protocols /
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| Edition: | Second edition. |
|---|---|
| Imprint: | New York : Humana Press, [2014] ©2014 |
| Description: | 1 online resource (xi, 369 pages) : illustrations (some color) |
| Language: | English |
| Series: | Methods in molecular biology, 1940-6029 ; Volume No. 1179 Methods in molecular biology (Clifton, N.J.) ; v. 1179. |
| Subject: | |
| Format: | E-Resource Book |
| URL for this record: | http://pi.lib.uchicago.edu/1001/cat/bib/11276162 |
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| 245 | 0 | 0 | |a Directed evolution library creation : |b methods and protocols / |c edited by Elizabeth M.J. Gillam, Janine N. Copp, David F. Ackerley. |
| 250 | |a Second edition. | ||
| 264 | 1 | |a New York : |b Humana Press, |c [2014] | |
| 264 | 4 | |c ©2014 | |
| 300 | |a 1 online resource (xi, 369 pages) : |b illustrations (some color) | ||
| 336 | |a text |b txt |2 rdacontent | ||
| 337 | |a computer |b c |2 rdamedia | ||
| 338 | |a online resource |b cr |2 rdacarrier | ||
| 490 | 1 | |a Methods in molecular biology, |x 1940-6029 ; |v Volume No. 1179 | |
| 504 | |a Includes bibliographical references and index. | ||
| 505 | 0 | 0 | |g PART I. |t POINT MUTAGENESIS -- |g 1. |t Error-Prone PCR and Effective Generation of Gene Variant Libraries for Directed Evolution / |r Janine N. Copp, Paulina Hanson-Manful, David F. Ackerley, and Wayne M. Patrick -- |g 2. |t Error-Prone Rolling Circle Amplification Greatly Simplifies Random Mutagenesis / |r Ryota Fujii, Motomitsu Kitaoka, and Kiyoshi Hayashi -- |g 3. |t Random Mutagenesis by Error-Prone Pol Plasmid Replication in Escherichia coli / |r David L. Alexander, Joshua Lilly, Jaime Hernandez, Jillian Romsdahl, Christopher J. Troll, and Manel Camps -- |g 4. |t The Sequence Saturation Mutagenesis (SeSaM) Method / |r Anna Joëlle Ruff, Tsvetan Kardashliev, Alexander Dennig, and Ulrich Schwaneberg -- |g 5. |t Generation of Effective Libraries by Neutral Drift / |r Miriam Kaltenbach and Nobuhiko Tokuriki -- |g 6. |t Site-Saturation Mutagenesis by Overlap Extension PCR / |r Elsie M. Williams, Janine N. Copp, and David F. Ackerley -- |g 7. |t Iterative Saturation Mutagenesis: A Powerful Approach to Engineer Proteins by Systematically Simulating Darwinian Evolution / |r Carlos G. Acevedo-Rocha, Sabrina Hoebenreich, and Manfred T. Reetz -- |g 8. |t Generating Targeted Libraries by the Combinatorial Incorporation of Synthetic Oligonucleotides During Gene Shuffling (ISOR) / |r Liat Rockah-Shmuel, Dan S. Tawfik, and Moshe Goldsmith -- |g 9. |t OmniChange: Simultaneous Site Saturation of Up to Five Codons / |r Alexander Dennig, Jan Marienhagen, Anna Joëlle Ruff, and Ulrich Schwaneberg -- |g 10. |t Random Insertional-Deletional Strand Exchange Mutagenesis (RAISE): A Simple Method for Generating Random Insertion and Deletion Mutations / |r Rota Fujii, Motomitsu Kitaoka, and Kiyoshi Hayashi -- |g 11. |t Transposon-Based Approaches for Generating Novel Molecular Diversity During Directed Evolution / |r D. Dafydd Jones, James A.J. Arpino, Amy J. Baldwin, and Matthew C. Edmundson. |
| 505 | 8 | 0 | |g PART II. |t RECOMBINATORIAL METHODS -- |g 12. |t Restriction Enzyme-Mediated DNA Family Shuffling / |r James B.Y.H. Behrendorff, Wayne A. Johnston, and Elizabeth M.J. Gillam -- |g 13. |t Assembly of Designed Oligonucleotides: A Useful Tool in Synthetic Biology for Creating High-Quality Combinatorial DNA Libraries / |r Carlos G. Acevedo-Rocha and Manfred T. Reetz -- |g 14. |t One-Pot Simple Methodology for Cassette Randomization and Recombination for Focused Directed Evolution (OSCARR) / |r Aurelio Hidalgo, Anna Schließmann, and Uwe T. Bornscheuer -- |g 15. |t USER Friendly DNA Recombination (USERec): Gene Library Construction Requiring Minimal Sequence Homology / |r Benoit Villiers and Florian Hollfelder -- |g 16. |t ITCHY: Incremental Truncation for the Creation of Hybrid Enzymes / |r Wayne M. Patrick and Monica L. Gerth -- |g 17. |t Generating Random Circular Permutation Libraries / |r Stefan Lutz, Ashley B. Daugherty, Ying Yu, and Zhen Qian. |
| 505 | 8 | 0 | |g PART III. |t STRUCTURE-GUIDED METHODS AND COMPUTATIONAL TOOLS FOR THE DESIGN AND ANALYSIS OF MUTANT LIBRARIES -- |g 18. |t Probabilistic Methods in Directed Evolution: Library Size, Mutation Rate, and Diversity / |r Yuval Nov -- |g 19. |t The Mutagenesis Assistant Program / |r Rajni Verma, Tuck Seng Wong, Ulrich Schwaneberg, and Danilo Roccatano -- |g 20. |t Computational Tools for Designing Smart Libraries / |r Eva Sebestova, Jaroslav Bendl, Jan Brezovsky, and Jiri Damborsky -- |g 21. |t Computational Tools for Directed Evolution: A Comparison of Prospective and Retrospective Strategies / |r Julian Zaugg, Yosephine Gumulya, Elizabeth M.J. Gillam, and Mikael Bodén -- |g 22. |t Designing Libraries of Chimeric Proteins Using SCHEMA Recombination and RASPP / |r Matthew A. Smith and Frances H. Arnold -- |g 23. |t Noncontiguous SCHEMA Protein Recombination / |r Matthew A. Smith and Frances H. Arnold -- |g 24. |t Engineering Proteins by Reconstructing Evolutionary Adaptive Paths / |r Vanessa E. Cox and Eric A. Gaucher. |
| 520 | 3 | |a Directed Evolution Library Creation: Methods and Protocols, Second Edition presents user-friendly protocols for both proven strategies and cutting-edge approaches for the creation of mutant gene libraries for directed evolution. | |
| 520 | 3 | |a As well as experimental methods, information on current computational approaches is provided in a user-friendly format that will allow researchers to make informed choices without needing to comprehend the full technical details of each algorithm. | |
| 520 | 3 | |a Directed evolution has become a fundamental approach for engineering proteins to enhance activity and explore structure-function relationships, and has supported the rapid development of the field of synthetic biology over the last decade. | |
| 520 | 3 | |a Divided into three convenient sections, topics include point mutagenesis strategies, recombinatorial methods wherein genetic diversity is sourced from multiple parental genes that are combined via either homology-dependent or -independent techniques, and a variety of computational methods to guide the design and analysis of mutant libraries. | |
| 520 | 3 | |a Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. | |
| 520 | 3 | |a Authoritative and easily accessible, Directed Evolution Library Creation: Methods and Protocols, Second Edition will serve as a reliable manual for both novice and experienced protein engineers and synthetic biologists and will enable further technical innovation and the exploitation of directed evolution for a deeper understanding of protein design and function. | |
| 650 | 0 | |a Gene libraries |v Laboratory manuals. | |
| 650 | 0 | |a Genetic engineering |v Laboratory manuals. | |
| 650 | 0 | |a Molecular evolution |v Laboratory manuals. | |
| 650 | 0 | |a Microbial mutation |v Laboratory manuals. | |
| 650 | 0 | |a Combinatorial chemistry |v Laboratory manuals. | |
| 650 | 0 | |a Genetic recombination |v Laboratory manuals. | |
| 650 | 1 | 2 | |a Directed Molecular Evolution. |
| 650 | 2 | 2 | |a Combinatorial Chemistry Techniques. |
| 650 | 2 | 2 | |a Gene Library. |
| 650 | 7 | |a Gene libraries |2 fast |0 (OCoLC)fst00939623 | |
| 650 | 7 | |a Genetic engineering |2 fast |0 (OCoLC)fst00940027 | |
| 650 | 7 | |a Molecular evolution |2 fast |0 (OCoLC)fst01024792 | |
| 650 | 7 | |a Microbial mutation |2 fast |0 (OCoLC)fst01019534 | |
| 650 | 7 | |a Combinatorial chemistry |2 fast |0 (OCoLC)fst00868966 | |
| 650 | 7 | |a Genetic recombination |2 fast |0 (OCoLC)fst00940079 | |
| 650 | 7 | |a Biology |x Laboratory manuals |2 fast |0 (OCoLC)fst00832428 | |
| 653 | 0 | 0 | |a evolutie |
| 653 | 0 | 0 | |a evolution |
| 653 | 0 | 0 | |a biomedische wetenschappen |
| 653 | 0 | 0 | |a biomedicine |
| 653 | 0 | 0 | |a genetische modificatie |
| 653 | 0 | 0 | |a genetic engineering |
| 653 | 0 | 0 | |a humane genetica |
| 653 | 0 | 0 | |a human genetics |
| 653 | 1 | 0 | |a Medicine (General) |
| 653 | 1 | 0 | |a Geneeskunde (algemeen) |
| 655 | 4 | |a Laboratory Manual. | |
| 700 | 1 | |a Gillam, Elizabeth M., |e editor |4 edt | |
| 700 | 1 | |a Copp, Janine N., |e editor |4 edt | |
| 700 | 1 | |a Ackerley, David F., |e editor |4 edt | |
| 776 | 0 | 8 | |i Print version: |t Directed evolution library creation. |b Second edition. |d Berlin : Springer, 2014 |z 9781493910526 |w (DLC) 2014943737 |w (OCoLC)885447740 |
| 830 | 0 | |a Methods in molecular biology (Clifton, N.J.) ; |v v. 1179. |x 1064-3745 | |
| 903 | |a HeVa | ||
| 929 | |a oclccm | ||
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| 928 | |t Library of Congress classification |a QH442.4 .D57 2014 |l Online |c UC-FullText |u https://link.springer.com/10.1007/978-1-4939-1053-3 |z Springer Nature |g ebooks |i 12532052 | ||