Bacterial pathogenesis. Part A, Identification and regulation of virulence factors /

Bacterial pathogenesis. Part A, Identification and regulation of virulence factors /

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Bibliographic Details
Imprint:San Diego : Academic Press, ©1994.
Description:1 online resource (xxx, 789 pages) : illustrations.
Language:English
Series:Methods in enzymology, 0076-6879 ; v. 235
Methods in enzymology ; v. 235.
Subject:
Pathogenic bacteria.
Virulence (Microbiology)
Bacterial diseases -- Animal models.
Pathogenic bacteria.
Virulence (Microbiology)
Format: E-Resource Book
URL for this record:http://pi.lib.uchicago.edu/1001/cat/bib/11278032
Hidden Bibliographic Details
Other authors / contributors:Clark, Virginia L., 1945-
Bavoil, Patrik M.
ISBN:0121821366
9780121821364
Notes:Includes bibliographical references and indexes.
Print version record.
Summary:This volume of Methods in Enzymology presents methods for the isolation and identification of bacterial pathogens and associated virulence determinants. It will enable the researcher to determine if closed genes or isolated gene products are involved in virulence. Major Topics Covered Include * Animal model system to determine bacterial virulence * Epidemiological techniques for the identification of bacterial strains and species * Protocols for the purification of subcellular bacterial products frequently associated with virulence * Determination of the means by which bacteria acquire iron * Methods to generate mutants, to construct isogenic strains by allelic exchange, to identify bacterial genes only expressed during infection of the host, and to study regulation of selected bacterial virulence factors * Methods for the assay of destructive bacterial enzymes and toxins.
Other form:Print version: Bacterial pathogenesis. Part A, Identification and regulation of virulence factors. San Diego : Academic Press, ©1994 0121821366
Table of Contents:
  • Recommendations for Working with Pathogenic Bacteria
  • Evaluations of Virulence in Animal Models
  • Determination of Median Lethal and Infectious Doses in Animal Model Systems
  • Experimental Keratoconjunctivitis (Sereny) Assay
  • Mouse Respiratory Infection Models for Pertussis
  • Chinchilla Model of Experimental Otitis Media for Study of Nontypable Haemophilus influenzae Vaccine Efficacy
  • Animal Models for Ocular Infections
  • Animal Models for Urogenital Infections
  • Animal Models for Meningitis
  • Animal Models for Periodontal Disease
  • Animal Chamber Models for Study of Host-Parasite Interactions
  • Animal Models for Immunoglobulin A Secretion
  • Species and Strain Identification
  • Serogroup and Serotype Classification of Bacterial Pathogens
  • Analysis of Genetic Variation by Polymerase Chain Reaction-Based Nucleotide Sequencing
  • Determinations of Restriction Fragment Length Polymorphism in Bacteria Using Ribosomal RNA Genes
  • DNA Fingerprintingof Mycobacterium tuberculosis
  • Phylogenetic Identification of Uncultured Pathogens Using Ribosomal RNA Sequences
  • Bacterial Cell Fractionation
  • Isolation of Outer Membranes
  • Isolation and Purification of Periplasmic Binding Proteins
  • Isolation and Characterization of Lipopolysaccharides, Lipooligosaccharides, and Lipid A
  • Isolation of Peptidoglycan and Soluble Peptidoglycan Fragments
  • Purification of treptococcal M Protein
  • Isolation and Assay of Pseudomonas aeruginosa Alginate
  • Purification of Escherichia
  • Microbial Acquisition of Iron
  • Deferration of Laboratory Media and Assays for Ferric and Ferrous Ions
  • Detection, Isolation, and Characterization of Siderophores
  • Effects of Iron Deprivation on Outer Membrane Protein Expression
  • Identification and Isolation of Mutants Defective in Iron Acquisition
  • Identification of Receptor-Mediated Transferrin-Iron Uptake Mechanism in Neisseria gonorrhoeae
  • Isolation of Genes Involved in Iron Acquisition by Cloning and Complementation of Escherichia
  • Genetics and Regulation
  • Bacterial Transformation by Electroporation
  • Analysis and Construction of Stable Phenotypes in Gram-Negative Bacteria with Tn5- and Tn10-Derived Minitransposons
  • U

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