Dendritic cell protocols.
Edition: | 2nd ed. / edited by Shalin H. Naik. |
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Imprint: | New York, N.Y. : Humana Press, ©2010. |
Description: | 1 online resource (xvii, 446 pages) : illustrations (some color) |
Language: | English |
Series: | Springer protocols Methods in molecular biology, 1940-6029 ; 595 Springer protocols (Series) Methods in molecular biology (Clifton, N.J.) ; v. 595. |
Subject: | |
Format: | E-Resource Book |
URL for this record: | http://pi.lib.uchicago.edu/1001/cat/bib/11281169 |
Summary: | Given the vital importance of immune system research, the gathering of clear, consistent, and informative protocols involving the study of dendritic cells is paramount. Bringing the popular first edition fully up to date, Dendritic Cell Protocols, Second Edition presents protocols from experts in the field that cover the basics and more complex forays into the exploration of DC development and function, both in mice and humans. The first section of the volume involving humans explores topics such as the isolation of blood DC subtypes, primary skin Langerhans cells, and the generation of gene-manipulated human DCs with the inclusion of more clinically relevant methods as well, while the second section involving rodent models delves into DC and precursor generation in vitro, isolation ex vivo, disease models, as well as DC functions and properties. Written in the highly successful Methods in Molecular Biology(tm) series style, chapters include introductions to their respective subjects, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls. Comprehensive and cutting-edge, Dendritic Cell Protocols, Second Edition aims to become a bench-side handbook for both beginners and experts in the field of DC research and a long-term reference for some of the most popular methods put forward by those who lead the field. |
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Item Description: | Title from PDF title page (SpringerLink, viewed December 7, 2009). |
Physical Description: | 1 online resource (xvii, 446 pages) : illustrations (some color) |
Bibliography: | Includes bibliographical references and index. |
ISBN: | 9781607614210 1607614219 9781607614203 1607614200 |
ISSN: | 1940-6029 ; |