In vitro mutagenesis protocols /
Edition: | 3rd ed. |
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Imprint: | [Totowa, N.J.] : Humana Press, ©2010. |
Description: | 1 online resource (xiv, 442 pages) : illustrations |
Language: | English |
Series: | Methods in molecular biology, 1064-3745 ; v. 634 Springer protocols Methods in molecular biology (Clifton, N.J.) ; volume 634. Springer protocols (Series) |
Subject: | |
Format: | E-Resource Book |
URL for this record: | http://pi.lib.uchicago.edu/1001/cat/bib/11283251 |
Summary: | In the post-genomic era, in vitro mutagenesis has emerged as a critically important tool for establishing the functions of components of the proteome. The third edition of In Vitro Mutagenesis Protocols represents a practical toolbox containing protocols vital to advancing our understanding of the connection between nucleotide sequence and sequence function. Fully updated from the previous editions, this volume contains a variety of specialty tools successfully employed to unravel the intricacies of protein-protein interaction, protein structure-function, protein regulation of biological processes, and protein activity, as well as a novel section on mutagenesis methods for unique microbes as a guide to the generalization of mutagenesis strategies for a host of microbial systems. Written in the highly successful Methods in Molecular Biology(tm) series format, chapters include brief introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and expert tips on troubleshooting and avoiding known pitfalls. Authoritative and up-to-date, In Vitro Mutagenesis Protocols, Third Edition offers today's researchers a valuable compendium of reliable and powerful techniques with which to illuminate the proteome and its rich web of biological implications. |
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Physical Description: | 1 online resource (xiv, 442 pages) : illustrations |
Bibliography: | Includes bibliographical references and index. |
ISBN: | 9781607616528 1607616521 9781607616511 1607616513 |
ISSN: | 1064-3745 ; |